Burkholderia contaminans Bacteriophage CSP3 Requires O-Antigen Polysaccharides for Infection

Stanton, Cassandra R.; Batinovic, Steven; Petrovski, Steve

doi:10.1128/spectrum.05332-22

Cited by 3 publications

References 85 publications

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Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages

Parra,

Lutz,

Brøndsted

et al. 2024

Microb Ecol

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Antimicrobial resistance (AMR) is a major public health threat, exacerbated by the ability of bacteria to rapidly disseminate antimicrobial resistance genes (ARG). Since conjugative plasmids of the incompatibility group P (IncP) are ubiquitous mobile genetic elements that often carry ARG and are broad-host-range, they are important targets to prevent the dissemination of AMR. Plasmid-dependent phages infect plasmid-carrying bacteria by recognizing components of the conjugative secretion system as receptors. We sought to isolate plasmid-dependent phages from wastewater using an avirulent strain of Salmonella enterica carrying the conjugative IncP plasmid pKJK5. Irrespective of the site, we only obtained bacteriophages belonging to the genus Alphatectivirus. Eleven isolates were sequenced, their genomes analyzed, and their host range established using S. enterica, Escherichia coli, and Pseudomonas putida carrying diverse conjugative plasmids. We confirmed that Alphatectivirus are abundant in domestic and hospital wastewater using culture-dependent and culture-independent approaches. However, these results are not consistent with their low or undetectable occurrence in metagenomes. Therefore, overall, our results emphasize the importance of performing phage isolation to uncover diversity, especially considering the potential of plasmid-dependent phages to reduce the spread of ARG carried by conjugative plasmids, and to help combat the AMR crisis.

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Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages

Parra,

Lutz,

Brøndsted

et al. 2024

Microb Ecol

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Isolation and characterization of bacteriophages against Burkholderia species

Shetty,

Rai,

Raj

et al. 2024

Preprint

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Burkholderia cepacia complex (Bcc) is a potent nosocomial pathogen mainly encountered in immunocompromised, cystic fibrosis and chronic granulomatous patients. They are innately resistant to the broad spectrum of antibiotics and thus need an alternative therapeutic approach, one of which is phage therapy. Therefore, our study aimed at isolating the bacteriophages against Burkholderia spp isolated from non-cystic fibrosis patients and characterizing these bacteriophages for therapeutic intervention. Initially, the hospital and environmental effluents were collected and screened for the presence of bacteriophages against Burkholderia spp. The obtained phages were then enriched and characterized by RAPD. Host range activity of phages were evaluated, and the cocktails of phages were screened for their synergistic effect. The morphology of the bacteriophages was examined using Transmission Electron Microscopy (TEM). Four bacteriophages were isolated and characterised against three different strains of Burkholderia. These, four phages were found to be strain specific and they did not show lytic activity against other species of Gram-negative isolates during host range analysis. Cocktail ;lassay –denoted that cocktail A showed broad host range activity against multiple species of Burkholderia. None of the phages showed any banding pattern for RAPD assay. TEM analysis revealed that phages belonged to Straboviridae family. The isolated phages against Burkholderia spp were found to be strain specific and active against Burkholderia cenocepacia, Burkholderia vietnamiensis and Burkholderia pseudomultivorans. This study has been a modest attempt to isolate bacteriophages against different genomovars of Burkholderia. The lytic activity of phages observed against the host determines the narrow spectrum activity of these phages and supports the theory of bacteriophage being one of the best alternatives for personalised clinical treatment of bacterial infections.

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Phage therapy to treat cystic fibrosis Burkholderia cepacia complex lung infections: perspectives and challenges

Canning,

Laucirica,

Ling

et al. 2024

Front. Microbiol.

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Burkholderia cepacia complex is a cause of serious lung infections in people with cystic fibrosis, exhibiting extremely high levels of antimicrobial resistance. These infections are difficult to treat and are associated with high morbidity and mortality. With a notable lack of new antibiotic classes currently in development, exploring alternative antimicrobial strategies for Burkholderia cepacia complex is crucial. One potential alternative seeing renewed interest is the use of bacteriophage (phage) therapy. This review summarises what is currently known about Burkholderia cepacia complex in cystic fibrosis, as well as challenges and insights for using phages to treat Burkholderia cepacia complex lung infections.

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Burkholderia contaminans Bacteriophage CSP3 Requires O-Antigen Polysaccharides for Infection

Abstract: Amid the global antibiotic resistance crisis, novel antimicrobials are needed to treat problematic bacterial infections, including those from the Burkholderia cepacia complex. One such alternative is the use of bacteriophages; however, a lot is still unknown about their biology.

Cited by 3 publications

References 85 publications

Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages

Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages

Isolation and characterization of bacteriophages against Burkholderia species

Phage therapy to treat cystic fibrosis Burkholderia cepacia complex lung infections: perspectives and challenges

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