Bupleurum chinense Polysaccharide Improves LPS-Induced Senescence of RAW264.7 Cells by Regulating the NF-κB Signaling Pathway

Abstract

Macrophages are important inflammatory cells that play a vital role in inflamm-aging. Bupleurum chinense polysaccharide (BCP), an effective component of the Bupleurum chinense herb, exerts multiple beneficial pharmacological effects, such as improving immunity and antioxidant activity. However, the effects of BCP on macrophage-aging and inflamm-aging are yet to be established. In this study, we examined the effects of BCP on proliferation, inflammatory cytokines, β-galactosidase (SA-β-gal), senescence-associated heterochromatin foci (SAHF), reactive oxygen species (ROS), mitochondrial membrane potential, p53, p16, and p65/NF-κB signaling proteins in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. BCP significantly inhibited production of interleukin-1α (IL-1α), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), reduced the expression of SA-β-gal and formation of SAHF, as well as ROS level, and stabilized the mitochondrial membrane potential in RAW264.7 cells stimulated with LPS. Furthermore, BCP inhibited the expression of aging-related genes, p53 and p16, suppressed phosphorylation of p65 protein, and enhanced the expression of I-κBα protein through the NF-κB signaling pathway in LPS-stimulated RAW264.7 cells. Accordingly, we conclude that BCP effectively suppresses inflamm-aging by reducing inflammatory cytokine levels and oxidative stress production following activation of the NF-κB signaling pathway in RAW264.7 cells stimulated with LPS. Our collective findings support the utility of BCP as a novel pharmaceutical agent with potential anti-inflamm-aging effects.

Comparative Study on Anti-Inflammatory Effect of Polysaccharides from Vinegar-Baked Radix Bupleuri Using Different Methods

“…Although the monosaccharide types of different VBCPs are similar, the ratios are different, which provides the basis for different activities. Surprisingly, although VBCP-HW showed comparable activity with the data in the literature, 7 , 37 − 40 other methods such as UA, CA, and KA exhibited an excellent anti-inflammatory effect at the dose of 1 μg/mL, a far lower dose than that in the literature; additionally, the inhibiting ratio on TNF-α increased from 39% of VBCP-HW to 91.6% of VBCP-KA (1 μg/mL), which was slightly higher than that of the positive control. Meanwhile, as a typical anti-inflammatory cytokine, IL-10 plays a key role in innate immunity and is usually an immune-enhancing factor.…”

Advances in Plant Polysaccharides as Antiaging Agents: Effects and Signaling Mechanisms

“…113 Bupleurum chinense polysaccharides improve the LPS-induced senescence of RAW 264.7 cells by regulating the NF-κB signaling pathway. 114 DOPs could reduce proinflammatory cytokines (TNF-α, IL-6) and MDA levels and improve estradiol, SOD, GSH-Px, T-AOC, and IL-10 levels in serum and alleviate the damage caused by aging through the inhibition of the NF-κB and p53/Bcl-2-mediated signaling pathways, suggesting that DOP may be a promising drug for treating premature ovarian failure (POF) caused by natural aging in females. 115 Xu et al found that Schisandra chinensis (Turcz.)…”