Bupleurum chinense extract ameliorates an OVA-induced murine allergic asthma through the reduction of the Th2 and Th17 cytokines production by inactivation of NFκB pathway

Bui, Thi; Piao, Chun Hua; Song, Chang Ho; Shin, Hee Soon; Chai, Ok Hee

doi:10.1016/j.biopha.2017.04.133

Cited by 37 publications

(19 citation statements)

References 60 publications

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“…It has been suggested that Th1/Th2/Th17 cytokines imbalance is involved in many inflammatory pulmonary diseases including asthma, tuberculosis, and pneumonia. [11][12][13][14] However, the roles of Th1/Th2/ In this study, we found Th1/Th2/Th17-related cytokines were In our study, we found both NAC and DEX treatments can effectively decrease pro-inflammatory mediators of IFN-γ, IL-2, IL-6…”

Section: Discussionmentioning

confidence: 48%

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Co‐administration of N‐acetylcysteine and dexmedetomidine plays a synergistic effect on protection of LPS‐induced acute lung injury via correcting Th1/Th2/Th17 cytokines imbalance

Qitai

Lin

Chen

et al. 2019

Clin Exp Pharma Physio

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Recently both N‐acetylcysteine (NAC) and Dexmedetomidine (DEX) have shown emerging roles in protection of acute lung injury (ALI). However, how their protective roles work and whether they can provide synergistic effects in ALI remain unknown. Here we explored it from the hot research viewpoint of Th1/Th2/Th17 cytokines balance. Lipopolysaccharide (LPS)‐induced ALI was established and treated with NAC and/or DEX. Mice were divided into Sham group, ALI group, NAC group, DEX group and NAC+DEX group. Mice were sampled at 6, 12 and 24 hours after the model construction. Histopathology, wet to dry ratio and myeloperoxidase (MPO) activity were assessed in lung tissues. Protein concentration and cell count were assessed in bronchoalveolar lavage fluid (BALF). Th1/Th2/Th17 cytokines were assessed in plasma, BALF and lung homogenate. ALI‐induced lung morphological damage, edema and aberrant MPO activity can be attenuated by NAC or DEX and mostly by NAC+DEX. NAC with DEX significantly reduced ALI‐induced protein leakage and cell infiltration in BALF. Th1/Th2/Th17 cytokines imbalance aggravated with ALI progression. NAC, DEX and especially NAC+DEX can effectively correct these unbalanced cytokines. Galectin‐9 and Tim‐3 were transcriptionally up‐regulated in ALI. Combination of NAC with DEX obtained a maximum effect on decreasing Galectin‐9/Tim‐3 expression. In summary, Th1/Th2/Th17 cytokines imbalance is newly found to participate in LPS‐induced ALI. NAC or DEX administration can attenuate ALI by rebalancing Th1/Th2/Th17 cytokines. Their protective roles can be enhanced when co‐administration, because DEX may relieve the Galectin‐9/Tim‐3 axis‐mediated immune suppression.

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Section: Discussionmentioning

confidence: 48%

“…It has been suggested that Th1/Th2/Th17 cytokines imbalance is involved in many inflammatory pulmonary diseases including asthma, tuberculosis, and pneumonia . However, the roles of Th1/Th2/Th17 cytokines in LPS‐induced ALI are not yet abundantly investigated.…”

Section: Discussionmentioning

confidence: 99%

Co‐administration of N‐acetylcysteine and dexmedetomidine plays a synergistic effect on protection of LPS‐induced acute lung injury via correcting Th1/Th2/Th17 cytokines imbalance

Qitai

Lin

Chen

et al. 2019

Clin Exp Pharma Physio

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“…As a result, traditional therapies based on plants are currently being reassessed. A number of studies have confirmed the effectiveness of herbs, natural products, and herbal formulae for asthma therapy, such as Bupleurum [18], Carica papaya [19], Zerumbone [20], and Bu-Shen-Yi-Qi formulae [21]. M. dauricum DC.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Total Alkaloids from Menispermum dauricum against Airway Inflammation in Asthmatic Mice

Yin

You

et al. 2020

Planta Med

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Abstract Menispermum dauricum is widely used to treat respiratory inflammation, including laryngopharyngitis, tonsillitis, tracheitis, and bronchitis. Total alkaloids isolated from M. dauricum have shown a variety of beneficial bioactivities. However, available data on the effects of M. dauricum total alkaloids against allergic asthma has not been reported. In present study, the protective effect of M. dauricum total alkaloids was evaluated by using an ovalbumin-induced in vivo model of asthma. The asthma model was prepared by sensitizing and challenging mice with ovalbumin, and M. dauricum total alkaloids (100, 200, and 400 mg/kg) were administrated to asthmatic mice by gavage. Histopathological analysis of pulmonary changes was detected by hematoxylin and eosin, and periodic acid-schiff staining. Inflammatory cell counts were determined in bronchoalveolar lavage fluid. Total immunoglobulin E and ovalbumin-specific immunoglobulin E levels in serum, and T-helper 2 cytokines and chemokine levels in bronchoalveolar lavage fluid were detected by an ELISA. Histological results demonstrated that M. dauricum total alkaloids significantly attenuated pulmonary inflammation in asthmatic mice. M. dauricum total alkaloid treatment exhibited marked effects on asthmatic mice in reducing inflammatory cell counts, decreasing interleukin-4, interleukin-5, and interleukin-13 concentrations, and downregulating TNF-α and eotaxin levels in bronchoalveolar lavage fluid. In addition, M. dauricum total alkaloids could also inhibit the elevated serum levels of total immunoglobulin E and ovalbumin-specific immunoglobulin E. These findings confirmed that M. dauricum total alkaloids could suppress airway inflammation in ovalbumin-induced asthma through regulating the T-helper 2 response and chemokine level. M. dauricum total alkaloids may be a potential ethnopharmacological agent for asthmatic patients.

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“…It is characterized by chronic airway inflammation and bronchial asthma. A relatively more unified view reveals that asthma is a development process, in which epithelial cells, fibroblasts, dendritic cells, eosinophils, mastocytes, T lymphocytes and other inflammatory cells interact with constituent cells of the airway, and the secreted inflammatory mediators participate in the development of asthma ( 1 – 3 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the levels of Th9 cells and cytokines in the peripheral blood of mice with bronchial asthma

Tong

Liang

et al. 2018

Exp Ther Med

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The purpose of the study was to detect the level of T-helper type 9 (Th9) cells and the cytokine interleukin-9 (IL-9) in peripheral blood of mice with bronchial asthma, and to explore the relationship between the expression of Th9 cells and the pathogenesis of asthma. Thirty female-specific pathogen-free (SPF) Bagg' albino (BALB)/c mice were selected and randomly divided into the control group (n=15) and the bronchial observation group (n=15). Mice in the bronchial observation group were treated with ovalbumin (OVA) for sensitization and induction of a mouse model of asthma. The airway reactivity of mice was measured by a mouse pulmonary function apparatus using the non-invasive pulmonary impedance method. The proportions of Th9 cells in peripheral blood of mice in the two groups were detected using flow cytometry. Digital polymerase chain reaction (dPCR), enzyme-linked immunosorbent assay (ELISA) and western blot analysis were applied to detect the levels of IL-9 messenger ribonucleic acid (mRNA) and proteins in peripheral blood and lung tissues of mice in the two groups, respectively. Compared with that in the control group, the expression level of Th9 cells in the peripheral blood of mice in the observation group was significantly elevated (P<0.05), the expression level of IL-9 proteins in the peripheral blood was significantly increased (P<0.05), and the levels of IL-9 mRNA and proteins in lung tissues were significantly increased (P<0.05). The results show that the levels of Th9 cells and their cytokine IL-9 in the peripheral blood of mice with bronchial asthma are significantly increased, suggesting that Th9 cells play important roles in the pathogenesis of asthma.

show abstract

Bupleurum chinense extract ameliorates an OVA-induced murine allergic asthma through the reduction of the Th2 and Th17 cytokines production by inactivation of NFκB pathway

Cited by 37 publications

References 60 publications

Co‐administration of N‐acetylcysteine and dexmedetomidine plays a synergistic effect on protection of LPS‐induced acute lung injury via correcting Th1/Th2/Th17 cytokines imbalance

Co‐administration of N‐acetylcysteine and dexmedetomidine plays a synergistic effect on protection of LPS‐induced acute lung injury via correcting Th1/Th2/Th17 cytokines imbalance

Protective Effects of Total Alkaloids from Menispermum dauricum against Airway Inflammation in Asthmatic Mice

Analysis of the levels of Th9 cells and cytokines in the peripheral blood of mice with bronchial asthma

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