Bull sperm acrosome reaction induced by gamma-aminobutyric acid (GABA) is mediated by GABAergic receptors type A

Puente, M.A.; Tartaglione, C.M.; Ritta, Mónica N.

doi:10.1016/j.anireprosci.2011.07.001

Cited by 25 publications

(25 citation statements)

References 43 publications

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“…At the very least, P enhances hamster sperm hyperactivation through both Ca 2+ signals and protein phosphorylations [5, 6, 8, 9, 12, 16, 30]. Moreover, it has been suggested that GABA induces the AR and capacitation in human, ram, rat, guinea pig and bull spermatozoa [19, 21, 23, 33,34,35,36]. Although in these species it is suggested that the effects of GABA on the AR and capacitation are the same as those of P, the regulatory mechanisms vary.…”

Section: Discussionmentioning

confidence: 99%

“…Although in these species it is suggested that the effects of GABA on the AR and capacitation are the same as those of P, the regulatory mechanisms vary. In bull [36], ram [21] and rat spermatozoa [19, 33], GABA increases the AR and capacitation through a GABA A receptor, whereas another study reported that in rat spermatozoa, GABA stimulates the AR through the GABA A receptor but inhibits it through the GABA B receptor [23]. In human spermatozoa, it has been suggested that both the GABA A and GABA B receptors block stimulation of the AR by follicular fluid [34].…”

Section: Discussionmentioning

confidence: 99%

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Suppression of Progesterone-enhanced Hyperactivation in Hamster Spermatozoa by γ-aminobutyric Acid

Kon

Takei

Fujinoki

et al. 2014

Journal of Reproduction and Development

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It has been recently shown that mammalian spermatozoa were hyperactivated by steroids, amines and amino acids. In the present study, we investigated whether hyperactivation of hamster sperm is regulated by progesterone (P) and γ-aminobutyric acid (GABA). Although sperm hyperactivation was enhanced by P, GABA significantly suppressed P-enhanced hyperactivation in a dose-dependent manner. Suppression of P-enhanced hyperactivation by GABA was significantly inhibited by an antagonist of the GABAA receptor (bicuculline). Moreover, P bound to the sperm head, and this binding was decreased by GABA. Because the concentrations of GABA and P change in association with the estrous cycle, these results suggest that GABA and P competitively regulate the enhancement of hyperactivation through the GABAA receptor.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Suppression of Progesterone-enhanced Hyperactivation in Hamster Spermatozoa by γ-aminobutyric Acid

Kon

Takei

Fujinoki

et al. 2014

Journal of Reproduction and Development

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“…In rat sperm, GABA and progesterone accelerated the process of capacitation and hyperactivated motility, effects that were inhibited by bicuculline and picrotoxin (Jin et al 2009 ). Additionally, it has been shown that GABA can induce the AR (Puente et al 2011 ;Shi et al 1997 ) and also that GABA A receptors can modulate the response to progesterone in these cells (Hu et al 2002 ;Ritta et al 1998 ;Shi and Roldan 1995 ;Turner et al 1994 ).…”

Section: Gaba and Glycine Receptorsmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) and immunocytochemistry studies revealed the presence of GABA A and GABA B receptors in spermatogenic cells, and patch-clamp studies showed that GABA application to round spermatogenic cells induced an inward Cl − current (Kanbara et al 2011 ). The presence and function of GABA A receptors in sperm from different species have been explored by several laboratories (Jin et al 2009 ;Meizel 1997 ;Puente et al 2011 ;Wistrom and Meizel 1993 ). For example, Ritta et al established that this neurotransmitter plays a role in the regulation of motility in bovine and human sperm (Ritta et al 2004 ;Ritta et al 1998 ).…”

Section: Gaba and Glycine Receptorsmentioning

confidence: 99%

Cl− Channels and Transporters in Sperm Physiology

Treviño

Orta

Figueiras-Fierro

et al. 2014

Sexual Reproduction in Animals and Plants

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Spermatozoa must decode environmental and cellular cues to succeed in fertilization, and this process relies heavily on ion channels. New observations bring to light the relevant participation of Cl − channels and anion transporters in some of the main sperm functions. Here we review the evidence that indicates the participation of Cl − channels in motility, maturation, and the acrosome reaction (AR), and what is known about their molecular identity and regulation. Our better understanding of sperm anion transport will yield tools to handle some infertility problems, improve animal breeding and preserve biodiversity, and develop selective and secure male contraceptives.

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“…A number of studies have focused on the evaluation of acrosome status (Kitiyanant et al 2002;Puente et al 2011;Almadaly 2012), sperm capacitation and variations among different bull breeds (Demyda-Peyras et al 2012). There are also numerous studies concerned with the high variability in embryo production under in vitro conditions, using spermatozoa from different bulls, but only a few studies have investigated a potential relationship between the in vitro-induced acrosome reaction and in vivo fertility of bulls (Whitfield and doi: 10.17221/8437-VETMED Parkinson 1995;Januskauskas et al 2000;Birck et al 2010;Kumar et al 2014).…”

mentioning

confidence: 99%

Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

Rečková¹,

Machatkova²,

Máchal³

et al. 2015

Vet. Med.

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This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with or without heparin. The spermatozoa were evaluated in terms of acrosomal changes at 0, 3, 4, 5, 6 and 8 h of capacitation. Proportions of acrosome-reacted spermatozoa at 5 h and 0 h of capacitation were used for calculation of the heparin response index. Variations in the heparin response index were found among individual bulls. Based on the mean response index value of all bulls, they fell into three categories: bulls with greater, intermediate and no response to heparin (GRH, IRH and NRH, respectively). Differences in the heparin response index between the bull categories were significant (P < 0.05). Higher D7 and D8 embryo development rates were found in the IRH vs. NRH bulls (P < 0.05). In conclusion, this study shows that the spermatozoa of bulls with a greater or intermediate response to heparin appear to be most suitable for in vitro embryo production compared with spermatozoa of bulls with no response to heparin.

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Bull sperm acrosome reaction induced by gamma-aminobutyric acid (GABA) is mediated by GABAergic receptors type A

Cited by 25 publications

References 43 publications

Suppression of Progesterone-enhanced Hyperactivation in Hamster Spermatozoa by γ-aminobutyric Acid

Suppression of Progesterone-enhanced Hyperactivation in Hamster Spermatozoa by γ-aminobutyric Acid

Cl− Channels and Transporters in Sperm Physiology

Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

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