Building up the <i>Drosophila</i> eggshell: First of all the eggshell genes must be transcribed

Cavaliere, Valeria; Bernardi, Fabio; Romani, Patrizia; Duchi, Serena; Gargiulo, Giuseppe

doi:10.1002/dvdy.21625

Cited by 78 publications

(76 citation statements)

References 98 publications

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“…Novel and unanticipated signatures included the upregulation of genes involved in phototransduction [64], highly time-specific regulation of genes regulated by TOR and downregulation of chorion genes (see the electronic supplementary material, figure S5) [65]. In marked contrast to the HT, Abd profiles showed changes in genes related to fewer potential functions.…”

Section: Resultsmentioning

confidence: 99%

Sex peptide of Drosophila melanogaster males is a global regulator of reproductive processes in females

et al. 2012

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Seminal fluid proteins (Sfps) alter female behaviour and physiology and can mediate sexual conflict. In Drosophila melanogaster, a single Sfp, the sex peptide (SP), triggers remarkable post-mating responses in females, including altered fecundity, feeding, immunity and sexual receptivity. These effects can favour the evolutionary interests of males while generating costs in females. We tested the hypothesis that SP is an upstream master-regulator able to induce diverse phenotypes through efficient induction of widespread transcriptional changes in females. We profiled mRNA responses to SP in adult female abdomen (Abd) and headþthorax (HT) tissues using microarrays at 3 and 6 h following mating. SP elicited a rich, subtle signature of temporally and spatially controlled mRNAs. There were significant alterations to genes linked to egg development, early embryogenesis, immunity, nutrient sensing, behaviour and, unexpectedly, phototransduction. There was substantially more variation in the direction of differential expression across time points in the HT versus Abd. The results support the idea that SP is an important regulator of gene expression in females. The expression of many genes in one sex can therefore be under the influence of a regulator expressed in the other. This could influence the extent of sexual conflict both within and between loci.

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Section: Resultsmentioning

confidence: 99%

Sex peptide of Drosophila melanogaster males is a global regulator of reproductive processes in females

et al. 2012

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“…Their specification is initiated when the TGFα-like ligand Gurken (GRK) is secreted from the dorsal anterior cortex of the oocyte and signals through EGF receptors on the neighboring follicle cells (Chang et al, 2008;Neuman-Silberberg and Schupbach, 1993;Neuman-Silberberg and Schupbach, 1994;Queenan et al, 1997). The resulting gradient of EGFR activation controls a number of transcription factors, signaling molecules and effector genes required for eggshell morphogenesis (Cavaliere et al, 2008;Dobens and Raftery, 2000;Wu et al, 2008;Yakoby et al, 2008a). Several other pathways, including Decapentaplegic (DPP) and Notch, are also involved in this process (Deng and Bownes, 1997;Twombly et al, 1996;Ward et al, 2006), but their role is secondary to that of the EGFR pathway as the dorsal eggshell structures are completely abolished in the absence of GRK (Schupbach, 1987).…”

Section: Introductionmentioning

confidence: 99%

Feedback control of the EGFR signaling gradient: superposition of domain-splitting events inDrosophilaoogenesis

et al. 2009

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The morphogenesis of structures with repeated functional units, such as body segments and appendages, depends on multi-domain patterns of cell signaling and gene expression. We demonstrate that during Drosophila oogenesis, the two-domain expression pattern of Broad, a transcription factor essential for the formation of the two respiratory eggshell appendages, is established by a single gradient of EGFR activation that induces both Broad and Pointed, which mediates repression of Broad. Two negativefeedback loops provided by the intracellular inhibitors of EGFR signaling, Kekkon-1 and Sprouty, control the number and position of Broad-expressing cells and in this way influence eggshell morphology. Later in oogenesis, the gradient of EGFR activation is split into two smaller domains in a process that depends on Argos, a secreted antagonist of EGFR signaling. In contrast to the previously proposed model of eggshell patterning, we show that the two-domain pattern of EGFR signaling is not essential for specifying the number of appendages. Thus, the processes that define the two-domain patterns of Broad and EGFR activation are distinct; their actions are separated in time and have different effects on eggshell morphology.

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“…The following alleles were used in this study: FRT42D miR-7 Δ1 (a gift from R. W. Carthew, Northwestern University, Evanston, IL, USA), a null allele generated by excision of the miR-7 sequence, last two exons of bl and two exons of hillarin (Li and Carthew 2005), UAS-miR-7.140 (a gift from S. M. Cohen, Institute of Molecular and Cellular Biology, Singapore) (Stark et al, 2003), ttk69-RNAi (Vienna Drosophila RNAi Center), UAS-Ttk69 (Bloomington Stock Center BL7361), FRT82B ttk 1e11 (French et al, 2003) and -253/-39 lacZ (VM32E-lacZ) (Cavaliere et al, 2008). Notch reporters were E(spl)mβ-CD2 (de Celis et al, 1998), m7-lacZ (Assa-Kunik et al, 2007 and GbeL-lacZ (Furriols and Bray, 2001 Flies were maintained and raised at 25°C.…”

Section: Fly Stocks and Genetic Clonesmentioning

confidence: 99%

The microRNA miR-7 regulates Tramtrack69 in a developmental switch in Drosophila follicle cells

et al. 2013

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SUMMARYDevelopment in multicellular organisms includes both small incremental changes and major switches of cell differentiation and proliferation status. During Drosophila oogenesis, the follicular epithelial cells undergo two major developmental switches that cause global changes in the cell-cycle program. One, the switch from the endoreplication cycle to a gene-amplification phase, during which special genomic regions undergo repeated site-specific replication, is attributed to Notch downregulation, ecdysone signaling activation and upregulation of the zinc-finger protein Tramtrack69 (Ttk69). Here, we report that the microRNA miR-7 exerts an additional layer of regulation in this developmental switch by regulating Ttk69 transcripts. miR-7 recognizes the 3′ UTR of ttk69 transcripts and regulates Ttk69 expression in a dose-dependent manner. Overexpression of miR-7 effectively blocks the switch from the endocycle to gene amplification through its regulation of ttk69. miR-7 and Ttk69 also coordinate other cell differentiation events, such as vitelline membrane protein expression, that lead to the formation of the mature egg. Our studies reveal the important role miR-7 plays in developmental decision-making in association with signal-transduction pathways.

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Building up the Drosophila eggshell: First of all the eggshell genes must be transcribed

Cited by 78 publications

References 98 publications

Sex peptide of Drosophila melanogaster males is a global regulator of reproductive processes in females

Sex peptide of Drosophila melanogaster males is a global regulator of reproductive processes in females

Feedback control of the EGFR signaling gradient: superposition of domain-splitting events inDrosophilaoogenesis

The microRNA miR-7 regulates Tramtrack69 in a developmental switch in Drosophila follicle cells

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