Building Block Lactic Acid from Rice Husks and Agave Bagasse

Montipó, Sheila; Pedroso, Giovanni B.; Bevilaqua, Daiane B.; Prestes, Osmar D.; Corona-González, Rosa Isela; Martins, Ayrton F.

doi:10.1007/s12649-016-9554-9

Cited by 14 publications

(8 citation statements)

References 34 publications

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“…The same occurs with Agave syrup consisting of non‐structural carbohydrates and used as a sweetener . Other important value‐added products that have been obtained from Agave residues include enzymes, lactic acid and succinic acid . A. atrovirens fibers were used as a substrate for solid‐state fermentation for cellulase (exoglucanase, endoglucanase, and β‐glucosidase) production by Trichoderma asperellum .…”

Section: Biofuels and Value‐added Products From Agavementioning

confidence: 92%

“…Lactic acid (2‐hydroxypropionic acid) is a highly versatile chemical that has gained interest as a monomer of polylactic acid (a biodegradable polymer). Acid hydrolysis was used in A. tequilana bagasse, producing 15.1 g glucose/L, and then a fermentation process using Lactobacillus rhammosus achieved 15 g lactic acid/L after approximately 20 h …”

Section: Biofuels and Value‐added Products From Agavementioning

confidence: 99%

“…Figure shows a general scheme of a biochemical platform biorefinery based on Agave where a sustainable processing of biomass into liquid biofuels (ethanol or n‐butanol), gaseous biofuels (hydrogen and methane), and value‐added products (such as spirits, succinic acid, or lactic acid) occurred via biological conversion . This included different stages after harvesting, where the stems are removed and the leaves are cut (which can be left in the field to recycle nutrients) and cooked in autoclaves or diffusers.…”

Section: Introductionmentioning

confidence: 99%

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Recent developments in Agave performance as a drought‐tolerant biofuel feedstock: agronomics, characterization, and biorefining

Perez-Pimienta

Lopez-Ortega

Sánchez

2017

Biofuels Bioprod Bioref

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In recent years, Agave has shown its potential as a bioenergy feedstock with a higher land productivity (up to 42 ton/ha year) than traditional feedstocks. Other features are its adaptation mechanism to high temperatures and its resistance to drought. The agronomics of Agave in Mexico are discussed, including total land planted, inputs required, and the harvesting and transport costs. Heating values, mineral concentration, and carbohydrate and lignin content show the potential of the Agave species to compete with current bioenergy crops. Currently, the pre-treatment of Agave is the most widely studied stage in biofuels and value-added products, which include technologies capable of reducing its recalcitrance while removing xylan and/or lignin and reducing cellulose crystallinity, among other effects, to increase the overall yield in saccharifi cation and fermentation, which will be discussed as well. In addition to spirits and fi bers from industrial interests, different liquid (ethanol and n-butanol) and gaseous (methane and hydrogen) biofuels, including certain value-added products (enzymes, lactic acid, and succinic acid), can be obtained from Agave in high yields. The main objective of this review is to address the recent advances in the utilization of Agave as a bioenergy feedstock for biofuels and value-added products within a sustainable biorefi nery scheme.

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Section: Biofuels and Value‐added Products From Agavementioning

confidence: 92%

Section: Biofuels and Value‐added Products From Agavementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recent developments in Agave performance as a drought‐tolerant biofuel feedstock: agronomics, characterization, and biorefining

Perez-Pimienta

Lopez-Ortega

Sánchez

2017

Biofuels Bioprod Bioref

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“…The highest sugar yields obtained with HCl or H 2 SO 4 pre-treatment also resulted in one of the highest inhibitor loads observed, i.e. 2.0 or 3.6 g/L formic acid and 8.3 or 7.8 g/L acetic acid, respectively [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of the major fermentation inhibitors of recombinant 2G yeasts in diverse lignocellulose hydrolysates

Vanmarcke

Demeke

Foulquié-Moreno

et al. 2021

Biotechnol Biofuels

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Background Presence of inhibitory chemicals in lignocellulose hydrolysates is a major hurdle for production of second-generation bioethanol. Especially cheaper pre-treatment methods that ensure an economical viable production process generate high levels of these inhibitory chemicals. The effect of several of these inhibitors has been extensively studied with non-xylose-fermenting laboratory strains, in synthetic media, and usually as single inhibitors, or with inhibitor concentrations much higher than those found in lignocellulose hydrolysates. However, the relevance of individual inhibitors in inhibitor-rich lignocellulose hydrolysates has remained unclear. Results The relative importance for inhibition of ethanol fermentation by two industrial second-generation yeast strains in five lignocellulose hydrolysates, from bagasse, corn cobs and spruce, has now been investigated by spiking higher concentrations of each compound in a concentration range relevant for industrial hydrolysates. The strongest inhibition was observed with industrially relevant concentrations of furfural causing partial inhibition of both D-glucose and D-xylose consumption. Addition of 3 or 6 g/L furfural strongly reduced the ethanol titer obtained with strain MD4 in all hydrolysates evaluated, in a range of 34 to 51% and of 77 to 86%, respectively. This was followed by 5-hydroxymethylfurfural, acetic acid and formic acid, for which in general, industrially relevant concentrations caused partial inhibition of D-xylose fermentation. On the other hand, spiking with levulinic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid or vanillin caused little inhibition compared to unspiked hydrolysate. The further evolved MD4 strain generally showed superior performance compared to the previously developed strain GSE16-T18. Conclusion The results highlight the importance of individual inhibitor evaluation in a medium containing a genuine mix of inhibitors as well as the ethanol that is produced by the fermentation. They also highlight the potential of increasing yeast inhibitor tolerance for improving industrial process economics.

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“…Previous research on ATB valorization has been exclusively focused on the holocellulose bioconversion to biofuels or byproducts such as ethanol, hydrogen, methane, organic acids, polyhydroxybutyrate (PHB), etc. − Most of these works have applied physical, chemical, biological, or any pretreatment combination thereof in order to release sugars from the holocellulose fractions and get rid of the lignin fraction. , However, this lignin fraction accounts for up to 20% of ATB, representing a big challenge for the sustainability of future ATB biorefineries . Bioconversion of lignin into biofuels or chemicals is still a challenge due to the heterogeneity and chemical structure of lignin.…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of Agave Bagasse Lignin to Medium-Chain-Length Polyhydroxyalkanoates by Pseudomonas putida

Arreola-Vargas

et al. 2022

ACS Sustainable Chem. Eng.

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Inadequate disposal of Agave tequilana bagasse (ATB) brings environmental and economic issues to tequila producing regions. Recent works have proposed technologies for valorization of the polysaccharide’s fractions of ATB. However, lignin bioconversion has not been investigated due to its recalcitrant nature. Herein, a systematic pretreatment is proposed to release the lignocellulosic fractions and produce medium-chain-length polyhydroxyalkanoates (PHA) from the lignin fraction of ATB. Nuclear magnetic resonance (NMR) analyses revealed that ATB lignin contained 63% of β-O-4 interunit linkages, making this lignin more suitable for pretreatment and biodegradation compared to other lignins. Exploratory experiments revealed that two-stage fermentation is suitable for PHA production from ATB lignin using wild type and engineered Pseudomonas putida strains. PHA increased from 0.09 to 0.39 g/L compared to single batch fermentation. Further improvement to 0.76 g/L was possible by using a central composite design to optimize the inoculum, substrate, and nitrogen concentrations. Finally, PHA depolymerase gene phaZ was knocked out from P. putida and tested at optimal conditions, enabling a PHA titer of 0.97 g/L. Analyses by NMR and GC-MS confirmed lignin derivatives consumption and decrease of β-O-4 linkages. This study lays the foundations to enable agave lignin bioprocessing and opens new avenues for ATB biorefineries.

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Building Block Lactic Acid from Rice Husks and Agave Bagasse

Cited by 14 publications

References 34 publications

Recent developments in Agave performance as a drought‐tolerant biofuel feedstock: agronomics, characterization, and biorefining

Recent developments in Agave performance as a drought‐tolerant biofuel feedstock: agronomics, characterization, and biorefining

Identification of the major fermentation inhibitors of recombinant 2G yeasts in diverse lignocellulose hydrolysates

Bioconversion of Agave Bagasse Lignin to Medium-Chain-Length Polyhydroxyalkanoates by Pseudomonas putida

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