Buffer medium exchange in continuous cell and particle streams using ultrasonic standing wave focusing

Augustsson, Per; Åberg, Lena; Swärd-Nilsson, Ann-Margret; Laurell, Thomas

doi:10.1007/s00604-008-0084-4

Cited by 66 publications

(53 citation statements)

References 30 publications

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“…ACT methods have been implemented for bio-particle wash [95,47,96,84,125,92], trapping [125,92,[126][127][128][129][130][131][132][133][134] and separation [69,108,85,91,9,81,135,94,136,137]. Bio-particle washing relies on focusing the particles to the center of the channel, and out flowing the bio-particles collected at the center into another suspension medium.…”

Section: Applicationmentioning

confidence: 99%

“…Utilizing this concept, the bio-particles such as RBCs [95,47,96] and yeast [84] have been washed with a microfluidic device. The idea of acoustics based bio-particle trapping can be used in the applications of bio-particle enrichment (concentration) [125] as well as clarification of these bio-particles from the suspension [92] through entrapment at certain locations within the microchannels or trapping them in droplets [126]. Moreover, using trapping force, patterning of bio-particles at certain locations may also be utilized for the collection of bio-particles for bio-assaying applications [92,127].…”

Section: Applicationmentioning

confidence: 99%

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Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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a b s t r a c tMicrofluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combination of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manipulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.

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Section: Applicationmentioning

confidence: 99%

Section: Applicationmentioning

confidence: 99%

Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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“…13 On the other hand, ACP offers an ability to create forces in a wider portion of the micro-channel with a lower selectivity, which makes ACP a good candidate for cell manipulation applications such as cell washing. 10,[14][15][16][17] Therefore, for the application of automated cell separation systems where cell washing and cell separation is performed on a single device, the use of ACP (in cell washing) with DEP (in cell separation) may be a subtle and promising solution with superior results compared to single mode of separation devices.…”

Section: Introductionmentioning

confidence: 99%

An integrated acoustic and dielectrophoretic particle manipulation in a microfluidic device for particle wash and separation fabricated by mechanical machining

et al. 2016

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In this study, acoustophoresis and dielectrophoresis are utilized in an integrated manner to combine the two different operations on a single polydimethylsiloxane (PDMS) chip in sequential manner, namely, particle wash (buffer exchange) and particle separation. In the washing step, particles are washed with buffer solution with low conductivity for dielectrophoretic based separation to avoid the adverse effects of Joule heating. Acoustic waves generated by piezoelectric material are utilized for washing, which creates standing waves along the whole width of the channel. Coupled electro-mechanical acoustic 3D multi-physics analysis showed that the position and orientation of the piezoelectric actuators are critical for successful operation. A unique mold is designed for the precise alignment of the piezoelectric materials and 3D side-wall electrodes for a highly reproducible fabrication. To achieve the throughput matching of acoustophoresis and dielectrophoresis in the integration, 3D side-wall electrodes are used. The integrated device is fabricated by PDMS molding. The mold of the integrated device is fabricated using high-precision mechanical machining. With a unique mold design, the placements of the two piezoelectric materials and the 3D sidewall electrodes are accomplished during the molding process. It is shown that the proposed device can handle the wash and dielectrophoretic separation successfully. V C 2016 AIP Publishing LLC.

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“…At the end of the channel, the three streams branch out into three outlets and the species are collected in the middle stream, suspended in the new solvent. Augustsson et al 21 reported a strategy to perform buffer exchange of particle and cell suspensions between two medium. In their design, an acoustic chamber with multi-segments and multiple consecutive cross-flow junctions was used to deform the fluid streams and shift the interface from the pressure nodal line.…”

Section: Introductionmentioning

confidence: 99%

“…As the system is set up for miscible fluid streams with dissimilar properties, the effects of diffusion at the fluid interface on the ultrasonic pressure field and nodal line location will also be investigated. Compared to the elaborate setup used by Augustsson et al, 21 the current design is simpler, but it is only suited to handle a small volume flow.…”

Section: Introductionmentioning

confidence: 99%

Cell separation and transportation between two miscible fluid streams using ultrasound

2012

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This paper presents a two-stream microfluidic system for transporting cells or micro-sized particles from one fluid stream to another by acoustophoresis. The two fluid streams, one being the original suspension and the other being the destination fluid, flow parallel to each other in a microchannel. Using a half-wave acoustic standing wave across the channel width, cells or particles with positive acoustic contrast factors are moved to the destination fluid where the pressure nodal line lies. By controlling the relative flow rate of the two fluid streams, the pressure nodal line can be maintained at a specific offset from the fluid interface within the destination fluid. Using this transportation method, particles or cells of different sizes and mechanical properties can be separated. The cells experiencing a larger acoustic radiation force are separated and transported from the original suspension to the destination fluid stream. The other particles or cells experiencing a smaller acoustic radiation force continue flowing in the original solution. Experiments were conducted to demonstrate the effective separation of polystyrene microbeads of different sizes (3 lm and 10 lm) and waterborne parasites (Giardia lamblia and Cryptosporidium parvum). Diffusion occurs between the two miscible fluids, but it was found to have little effects on the transport and separation process, even when the two fluids have different density and speed of sound.

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Buffer medium exchange in continuous cell and particle streams using ultrasonic standing wave focusing

Cited by 66 publications

References 30 publications

Microfluidic bio-particle manipulation for biotechnology

Microfluidic bio-particle manipulation for biotechnology

An integrated acoustic and dielectrophoretic particle manipulation in a microfluidic device for particle wash and separation fabricated by mechanical machining

Cell separation and transportation between two miscible fluid streams using ultrasound

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