Buccal fat pad reconstruction for oral submucous fibrosis

Surej, Kumar L K; Kurien, Nikhil Mathew; Sakkir, Nasil

doi:10.4103/0975-5950.79222

Cited by 9 publications

(4 citation statements)

References 10 publications

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“…In addition, BFP is a discarded tissue in plastic surgery for cheek reduction [ 13 ]. Furthermore, it is routinely administered in the treatment of the bone, periodontal defects [ 49 , 55 – 59 ], congenital oroantral diseases, oronasal diseases [ 60 ], congenital cleft palate repair [ 61 ], oral submucous fibrosis [ 62 , 63 ], intraoral malignant defects [ 64 ], and cheek mucosa defects [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Buccal Fat Pad as a Potential Source of Stem Cells for Bone Regeneration: A Literature Review

Salehi

Rad

Kheiri

et al. 2017

Stem Cells International

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Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fat pad (BFP) is a specialized adipose tissue that is easy to harvest and contains a rich blood supply, and its harvesting causes low complications for patients. This review focuses on the characteristics and osteogenic capability of stem cells derived from BFP as a valuable cell source for bone tissue engineering. An electronic search was performed on all in vitro and in vivo studies that used stem cells from BFP for the purpose of bone tissue engineering from 2010 until 2016. This review was organized according to the PRISMA statement. Adipose-derived stem cells derived from BFP (BFPSCs) were compared with adipose tissues from other parts of the body (AdSCs). Moreover, the osteogenic capability of dedifferentiated fat cells (DFAT) derived from BFP (BFP-DFAT) has been reported in comparison with BFPSCs. BFP is an easily accessible source of stem cells that can be obtained via the oral cavity without injury to the external body surface. Comparing BFPSCs with AdSCs indicated similar cell yield, morphology, and multilineage differentiation. However, BFPSCs proliferate faster and are more prone to producing colonies than AdSCs.

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Section: Discussionmentioning

confidence: 99%

Buccal Fat Pad as a Potential Source of Stem Cells for Bone Regeneration: A Literature Review

Salehi

Rad

Kheiri

et al. 2017

Stem Cells International

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“…14 The buccal fat pad flaps are used in the reconstruction of the periorbital area, 15 for the repair of congenital oro-antral and/or oro-nasal disorders, 16 and for congenital cleft palate repair. 17 More recently, they have also been used for the closure of oro-antral communications 18,19 and treatment of oral submucous fibrosis, 20,21 intraoral malignant defects, 22 and cheek mucosa defects. 23 …”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells from Bichat's Fat Pad:In VitroComparison with Adipose-Derived Stem Cells from Subcutaneous Tissue

Broccaioli

Niada

Rasperini

et al. 2013

BioResearch Open Access

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Adipose-derived stem/stromal cells (ASCs) are progenitor cells used in bone tissue engineering and regenerative medicine. Since Bichat's fat pad is easily accessible for dentists and maxillo-facial surgeons, we compared the features of ASCs from Bichat's fat pad (BFP-ASCs) with human ASCs from subcutaneous adipose tissue (SC-ASCs). BFP-ASCs isolated from a small amount of tissue were characterized for their stemness and multidifferentiative ability. They showed an important clonogenic ability and the typical mesenchymal stem cell immunophenotype. Moreover, when properly induced, osteogenic and adipogenic differentiation markers, such as alkaline phosphatase activity, collagen deposition and lipid vacuoles formation, were promptly observed. Growth of both BFP-ASCs and SC-ASCs in the presence of human serum and their adhesion to natural and synthetic scaffolds were also assessed. Both types of ASCs adapted rapidly to human autologous or heterologous sera, increasing their proliferation rate compared to standard culture condition, and all the cells adhered finely to bone, periodontal ligament, collagen membrane, and polyglycol acid filaments that are present in the oral cavity or are commonly used in oral surgery. At last, we showed that amelogenin seems to be an early osteoinductive factor for BFP-ASCs, but not SC-ASCs, in vitro. We conclude that Bichat's fat pad contains BFP-ASCs with stemness features that are able to differentiate and adhere to biological supports and synthetic materials. They are also able to proliferate in the presence of human serum. For all these reasons we propose BFP-ASCs for future therapies of periodontal defects and bone regeneration.

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“…Since 1977, the BFP has been used in surgery for the treatment of congenital oroantral and/or oronasal diseases [15], congenital cleft palate repair [16], oral submucous fibrosis [17,18], intraoral malignant defects [19], and cheek mucosa defects [15,20]. In addition, BFP is a discarded tissue of plastic surgery for cheek reduction.…”

Section: Introductionmentioning

confidence: 99%

“…The BFP is located between the masseter and buccinators muscles and the ascending mandibular ramus and zygomatic arch [ 13 ], and it is easily accessible with a simple surgical procedure under local anesthesia [ 14 ]. Since 1977, the BFP has been used in surgery for the treatment of congenital oroantral and/or oronasal diseases [ 15 ], congenital cleft palate repair [ 16 ], oral submucous fibrosis [ 17 , 18 ], intraoral malignant defects [ 19 ], and cheek mucosa defects [ 15 , 20 ]. In addition, BFP is a discarded tissue of plastic surgery for cheek reduction.…”

Section: Introductionmentioning

confidence: 99%

Porcine adipose-derived stem cells from buccal fat pad and subcutaneous adipose tissue for future preclinical studies in oral surgery

et al. 2013

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IntroductionAdipose-derived stem cells (ASCs) are progenitor cells used in bone tissue engineering and regenerative medicine. Despite subcutaneous adipose tissue being more abundant, the buccal fat pad (BFP) is easily accessible for dentists and maxillofacial surgeons. For this reason, considering the need for preclinical study and the swine as an optimal animal model in tissue engineering applications, we compared the features of porcine ASCs (pASCs) from both tissue-harvesting sites.MethodsASCs were isolated from interscapular subcutaneous adipose tissue (ScI) and buccal fat pads of six swine. Cells were characterized for their stemness and multipotent features. Moreover, their osteogenic ability when cultured on titanium disks and silicon carbide-plasma-enhanced chemical vapor-deposition fragments, and their growth in the presence of autologous and heterologous serum were also assessed.ResultsIndependent of the harvesting site, no differences in proliferation, viability, and clonogenicity were observed among all the pASC populations. Furthermore, when induced toward osteogenic differentiation, both ScI- and BFP-pASCs showed an increase of collagen and calcified extracellular matrix (ECM) production, alkaline phosphatase activity, and osteonectin expression, indicating their ability to differentiate toward osteoblast-like cells. In addition, they differentiated toward adipocyte-like cells, and chondrogenic induced pASCs were able to increase glycosaminoglycans (GAGs) production over time. When cells were osteoinduced on synthetic biomaterials, they significantly increased the amount of calcified ECM compared with control cells; moreover, titanium showed the osteoinductive effect on pASCs, also without chemical stimuli. Finally, these cells grew nicely in 10% FBS, and no benefits were produced by substitution with swine serum.ConclusionsSwine buccal fat pad contains progenitor cells with mesenchymal features, and they also osteo-differentiate nicely in association with synthetic supports. We suggest that porcine BFP-ASCs may be applied in preclinical studies of periodontal and bone-defect regeneration.

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Buccal fat pad reconstruction for oral submucous fibrosis

Cited by 9 publications

References 10 publications

Buccal Fat Pad as a Potential Source of Stem Cells for Bone Regeneration: A Literature Review

Buccal Fat Pad as a Potential Source of Stem Cells for Bone Regeneration: A Literature Review

Mesenchymal Stem Cells from Bichat's Fat Pad:In VitroComparison with Adipose-Derived Stem Cells from Subcutaneous Tissue

Porcine adipose-derived stem cells from buccal fat pad and subcutaneous adipose tissue for future preclinical studies in oral surgery

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