BsmI but not FokI polymorphism of VDR gene is contributed in breast cancer

Abstract

There is growing evidence of a malignancy-protective role for vitamin D in breast cancer. The effects of vitamin D are mediated via the vitamin D receptor (VDR) which is encoded by VDR gene. Several SNPs on VDR gene has attracted research interest, although the magnitude of the impact of VDR allelic variations on breast cancer has been controversial. In the present study, we focused on the distribution of VDR FokI and BsmI polymorphisms in Iranian breast cancer patients. A case-control study was conducted on 296 samples including 140 breast cancer patients and 156 age matched control women. Restriction fragment length polymorphism (RFLP) analysis was performed for BsmI and FokI genotyping. Randomly selected PCR products were subjected to sequencing to verify the RFLP results. A significantly increased risk of breast cancer was observed with BsmI bb or even Bb genotype (OR 2.39, CI 1.17-4.85 and OR 2.28, CI 1.16-4.47, respectively). Nevertheless, statistically significant association between FokI genotypes and breast cancer risk was not observed. This study lends support for an increased risk of breast cancer associated with the VDR BsmI polymorphism.

Association Between VDR Polymorphisms and Breast Cancer: An Updated and Comparative Meta-analysis of Crude and Adjusted Odd Ratios

“…Case-control studies on Fok1 in breast cancer showed some evidence of increased risk among ff carriers (Sinotte et al, 2008;Gapska et al, 2009;McKay et al, 2009), which was confirmed by some later metaanalysis Tang et al, 2009;. However, these studies also reported some decreased risk among ff carriers (Anderson et al, 2011), or no association with breast cancer (Curran et al, 1999;Guy et al, 2004;John et al, 2007;Abbas et al, 2008;Engel et al, 2012;Rollison et al, 2012;Fuhrman et al, 2013;Mishra et al, 2013;Shahbazi et al, 2013). Similarly, mixed results have been observed concerning the relationship between other polymorphisms and the risk of breast cancer development.…”

“…2013; Shahbazi et al, 2013) but not in others (Buyru et al, 2003;Hefler et al, 2004;VandeVord et al, 2006;Trabert et al, 2007;Sinotte et al, 2008;Gapska et al, 2009;McKay et al, 2009;Anderson et al, 2011;Rollison et al, 2012;Mishra et al, 2013); Apa1 was found with positive relationship in some breast cancer studies (Curran et al, 1999;Sillanpaa et al, 2004;Dalessandri et al, 2012), but negative in others (Cui et al, 2001;Hou et al, 2002;Chakraborty et al, 2009;Anderson et al, 2011;Engel et al, 2012;Mishra et al, 2013); the same as Taq1, with associations in some studies (Cui et al, 2001;, but not in others (Curran et al, 1999;Dunning et al, 1999;Lundin et al, 1999;Hou et al, 2002;Newcomb et al, 2002;Buyru et al, 2003;Sillanpaa et al, 2004;John et al, 2007;Abbas et al, 2008;Chakraborty et al, 2009;Gapska et al, 2009;Anderson et al, 2011;Engel et al, 2012;Mishra et al, 2013); Cdx2, positive in some studies (Anderson et al, 2011;Yao et al, 2012; but not in others (Abbas et al, 2008;Zhou et ...…”

Do VDR Gene Polymorphisms Contribute to Breast Cancer?

“…In the year 2009, the meta-analysis was done by Tang et al, which shows a strong connection between VDR ff and raised risk of breast cancer in European females. Study by Shahbazi et al (2013) on Iranian breast cancer patients could not establish statistically significant affiliation between Fok1 genotypes and breast carcinoma. However in 2013, Mishra et al, proposed that VDR FokI f allele may share in the evolution of breast carcinoma in young age and F allele in tumor progression and patient prognosis.…”

“…The second most frequent studied VDR polymorphism-BsmI, which after analysis showed mixed results. Some studies reported its association with breast cancer development (Guy et al, 2004;Shahbazi et al, 2013).The study by Lowe et al (2005) shows strong association between the VDR bb genotype and breast cancer. Studies by other researchers did not reveal any significant difference in the prevalence of the BsmI polymorphism in breast cancer patients (Chen et al, 2005; DOI:http://dx.doi.org/10.7314/APJCP.2016.17.2.479 Do VDR Gene Polymorphisms Contribute to Breast Cancer?…”

Lack of an Association Between Vitamin D Receptor BsmI Gene Polymorphism and the Risk of End-Stage Renal Disease: A Meta-Analysis

“…It is well-documented that VDR BsmI gene polymorphism is associated with the risk of several cancers (18)(19)(20). In patients with chronic kidney disease, VDR BsmI gene polymorphism is reported to be associated with the onset of various complications, such as cardiovascular disease (10).…”