BSC-1 growth inhibitor transforms a mitogenic stimulus into a hypertrophic stimulus for renal proximal tubular cells: relationship to Na+/H+ antiport activity.

Fine, Leon G.; Holley, Robert W.; Nasri, Hamzeh; Badie-Dezfooly, B.

doi:10.1073/pnas.82.18.6163

Cited by 89 publications

(33 citation statements)

References 23 publications

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“…This ubiquitous growth factor functions in an autocrine or paracrine mode to elicit a multiplicity of cellular effects including inhibition of cellular proliferation and induction of tubuloepithelial cell hypertrophy (8). Our studies demonstrate that ANG II stimulates in MCT cells the production of active TGF-(3 as assessed by a bioassay using mink lung epithelial cells.…”

Section: Introductionmentioning

confidence: 73%

“…Only a few factors are hypertrophogenic for tubular cells in vitro. Such effectors are transforming growth factor-e (TGF-(3;' 8), high glucose content of the culture medium (9, 10), insulin-like growth factor 1 ( 11), and angiotensin II (ANG II; [12][13][14][15][16]. We (12)(13)(14)(15) and others ( 16) have investigated in a series of experiments the effects on ANG II in cultured proximal tubular cells.…”

Section: Introductionmentioning

confidence: 99%

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Angiotensin II-induced hypertrophy of cultured murine proximal tubular cells is mediated by endogenous transforming growth factor-beta.

Wolf

Mueller²,

Stahl³

et al. 1993

J. Clin. Invest.

366

186

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Previous studies by our group have demonstrated that angiotensin II (ANG II), as a single factor in serum-free medium, induces cellular hypertrophy of a cultured murine proximal tubular cell line (MCT). The present study was performed to test the hypothesis that this growth effect was mediated by activation of endogenous transforming growth factor-,@ (TGF-,B). Exogenous TGF-#1 (1 ng/ml) mimicked the growth effects observed with 10i-M ANG II (inhibition of DNA synthesis and induction of cellular hypertrophy). A neutralizing anti-TGF-ft antibody attenuated the ANG II-induced increase in de novo protein and total RNA synthesis as well as total protein content. This antibody also abolished the ANG II-mediated inhibition of 13HIthymidine incorporation into quiescent MCT cells.Control IgG or an unrelated antibody had no effect. A bioassay for TGF-ft using mink lung epithelial cells revealed that MC`'

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Section: Introductionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Angiotensin II-induced hypertrophy of cultured murine proximal tubular cells is mediated by endogenous transforming growth factor-beta.

Wolf

Mueller²,

Stahl³

et al. 1993

J. Clin. Invest.

366

186

View full text Add to dashboard Cite

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“…While stimulatory for fibroblasts and certain other mesenchymal cells, TGF-P is a growth inhibitor for most cell types (5,30). It is the most potent growth inhibitory polypeptide demonstrated so far (4), and it inhibits the proliferation oflymphoid cells (31,32), myeloid cells (30), and epithelial cells, including hepatocytes (10)(11)(12)(13)(14), keratinocytes (5, 6), bronchial epithelial cells (7), renal proximal tubular cells (8), and granulosa cells (9).…”

Section: Methodsmentioning

confidence: 99%

“…3) and is a potent growth inhibitor for cultured cells of epithelial origin, including hepatocytes (4)(5)(6)(7)(8)(9)(10)(11)(12)(13). Subpicomolar concentrations of TGF-,3 inhibit DNA synthesis stimulated by epidermal growth factor and other growth factors in primary cultures of adult rat (10)(11)(12)(13) and human fetal (14) hepatocytes and in lines of normal, but not of transformed, diploid rat liver epithelial cells (12,15).…”

mentioning

confidence: 99%

Type beta transforming growth factor reversibly inhibits the early proliferative response to partial hepatectomy in the rat.

Russell

Coffey

Ouellette

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

391

222

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Type (3 transforming growth factor (TGF-fi), a factor produced by many cell types, is a potent inhibitor of hepatocyte DNA synthesis in vitro. To determine whether TGF-fi can influence hepatocyte proliferation in vivo, its effects were examined on the regenerative response of liver to partial hepatectomy (PH) in the rat. Porcine platelet-derived (19,20).We studied the effect of intravenous porcine plateletderived TGF-f3, in amounts calculated to saturate hepatic TGF-,8 receptors, on the rate of DNA synthesis and the regeneration of liver mass induced by partial hepatectomy in the rat. We also studied the effects of TGF-,8 on liver morphology and blood concentrations of several liverderived or regulated substances. MATERIALS AND METHODSPartial Hepatectomy. Male Sprague-Dawley rats (Holtzman, Madison, WI) weighing 182 + 14 g (mean ± SD) were given free access to water and a complete liquid diet (Dyets, Bethlehem, PA). They were individually housed under controlled lighting (lights on 0600-1800 hr). Normal weight gain and stable food intake were documented before study. Because of the striking diurnal variation in liver DNA synthesis after partial hepatectomy documented by Barbiroli and Potter (21), all rats underwent partial hepatectomy (PH) or sham hepatectomy between 1100 and 1500 hr. PH consisted of excision of the median and left lateral lobes, which comprise 69.2% ± 0.6% of the total liver weight (19,20). To control for the nutritional effects of PH, the group of sham-operated animals was pair fed with the PH group. In the 22 hr following the operation, PH rats consumed 13.7 ± 9.3 kcal (1 cal = 4.18 J), compared with 52.4 ± 9.3 kcal in the 24 hr preceding PH. Sham hepatectomy consisted of liver manipulation without ablation; sham hepatectomy animals were allowed a total of 14 kcal in the 22 hr after operation.Porcine TGF-f3, forms 1 and 2 (TGF-,81, TGF-,B2; R&D Systems, Minneapolis, MN), at 1.0 ,ug/ml in 4 mM HCl with 1 mg of bovine serum albumin per ml (0.5 ml), was injected into a tail vein at the times indicated. Bovine serum albumin/ Abbreviations: TGF-,3, type ,B transforming growth factor; IGF-I, insulin-like growth factor I; PH, partial hepatectomy. tTo whom reprint requests should be addressed at: Pediatric Endocrine-Metabolic Unit, ACC 609,

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“…Alternatively, cell enlargement could be initiated and maintained by a specific set of events. Identification of autocrine inhibitory substances as regulators of proximal tubular cell growth in vitro (2) suggests that progression through the cell cycle could be blocked by such inhibitors. If the cell cycle is interrupted in hypertrophy, events preceding the blockade would be similar to those in dividing cells.…”

mentioning

confidence: 99%

Patterns of mRNA expression during early cell growth differ in kidney epithelial cells destined to undergo compensatory hypertrophy versus regenerative hyperplasia.

Norman

Bohman

Fischmann

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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Patterns of mRNA expression during early cell growth differ in kidney epithelial cells destined to undergo compensatory hypertrophy versus regenerative hyperplasia (unilateral Communicated by Jared M. Diamond, May 16, 1988 ABSTRACT An increase in cell size and protein content is characteristic of cells undergoing hypertrophy and of replicating cells prior to DNA synthesis. Cell enlargement in the two situations could be regulated by similar early events with an interruption of the cell cycle occurring in hypertrophy, or the two processes could be uncoupled. In vivo models were used to compare hypertrophy induced by unilateral nephrectomy and hyperplasia induced by folic acid injection in rabbit renal cortical cells. Within 48 hr, cell volume increased in both groups but the number of cells in the cell cycle and DNA synthesis was increased only after folic acid. Patterns of mRNA expression of the following three groups of cell cycle-dependent genes were analyzed: (i) protooncogenes (c-fos, c-myc, and c-Ha-ras), (ii) structural protein genes (vimentin and fi-actin), and (iii) transport protein genes (Na',K+-ATPase, ADP-ATP translocase, and calcyclin). mRNAs for all genes, except calcyclin and c-Ha-ras, were detected in controls. Folic acid generally induced rapid, transient increases in mRNA levels, but after unilateral nephrectomy, expression of most mRNAs showed a gradual, progressive increase. These data indicate that gene expression in the early stages of cell enlargement differs in cells destined to undergo proliferation vs. hypertrophy. The term "sustained message amplification" is proposed

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BSC-1 growth inhibitor transforms a mitogenic stimulus into a hypertrophic stimulus for renal proximal tubular cells: relationship to Na+/H+ antiport activity.

Cited by 89 publications

References 23 publications

Angiotensin II-induced hypertrophy of cultured murine proximal tubular cells is mediated by endogenous transforming growth factor-beta.

Angiotensin II-induced hypertrophy of cultured murine proximal tubular cells is mediated by endogenous transforming growth factor-beta.

Type beta transforming growth factor reversibly inhibits the early proliferative response to partial hepatectomy in the rat.

Patterns of mRNA expression during early cell growth differ in kidney epithelial cells destined to undergo compensatory hypertrophy versus regenerative hyperplasia.

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