BSA-FITC-loaded microcapsules for in vivo delivery

Kim, Byung Soo; Oh, Jae Min; Kim, Kyung Sook; Seo, Kwang Su; Cho, Jae Song; Khang, Gilson; Lee, Hai Bang; Park, Kinam; Kim, Moon Suk

doi:10.1016/j.biomaterials.2008.10.030

Cited by 73 publications

(41 citation statements)

References 33 publications

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“…Since FITC-BSA was widely used as a fluorescent marker to investigate the nanoparticle transport through biological barriers in vitro or in vivo, FITC-BSA was attached to the carboxyl groups of SWCNHox via diimide-activated amidation and used for tracing SWCNHox. 10,35,36 characterization of agsWcNhs, sWcNhox, and F-B-sWcNhox Morphology and eDs analysis TEM observation revealed that agSWCNHs and SWCNHox were spherical in shape ( Figure 1C), and that SWCNHox had holes in their walls. EDS results verified that the oxidation treatment did not introduce new elements to SWCNHs, and only changed the contents of existing elements ( Figure S1).…”

Section: Preparation Of Swcnhox and F-b-swcnhoxmentioning

confidence: 99%

The interactions of single-wall carbon nanohorns with polar epithelium

Shi¹,

Shi

Li³

et al. 2017

IJN

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Single-wall carbon nanohorns (SWCNHs), which have multitudes of horn interstices, an extensive surface area, and a spherical aggregate structure, offer many advantages over other carbon nanomaterials being used as a drug nanovector. The previous studies on the interaction between SWCNHs and cells have mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells. Polar epithelium as a typical biological barrier constitutes the prime obstacle for the transport of therapeutic agents to target site. This work tried to explore the permeability of SWCNHs through polar epithelium and their abilities to modulate transcellular transport, and evaluate the potential of SWCNHs in drug delivery. Madin-Darby canine kidney (MDCK) cell monolayer was used as a polar epithelial cell model, and as-grown SWCNHs, together with oxidized and fluorescein isothiocyanate-conjugated bovine serum albumin-labeled forms, were constructed and comprehensively investigated in vitro and in vivo. Various methods such as transmission electron microscopy and confocal imaging were used to visualize their intracellular uptake and localization, as well as to investigate the potential transcytotic process. The related mechanism was explored by specific inhibitors. Additionally, fast multispectral optoacoustic tomography imaging was used for monitoring the distribution and transport process of SWCNHs in vivo after oral administration in nude mice, as an evidence for their interaction with the intestinal epithelium. The results showed that SWCNHs had a strong bioadhesion property, and parts of them could be uptaken and transcytosed across the MDCK monolayer. Multiple mechanisms were involved in the uptake and transcytosis of SWCNHs with varying degrees. After oral administration, oxidized SWCNHs were distributed in the gastrointestinal tract and retained in the intestine for up to 36 h probably due to their surface adhesion and endocytosis into the intestinal epithelium. Overall, this comprehensive investigation demonstrated that SWCNHs can serve as a promising nanovector that can cross the barrier of polar epithelial cells and deliver drugs effectively.

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Section: Preparation Of Swcnhox and F-b-swcnhoxmentioning

confidence: 99%

The interactions of single-wall carbon nanohorns with polar epithelium

Shi¹,

Shi

Li³

et al. 2017

IJN

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“…Bovine serum albumin (BSA) in the outer aqueous phase is labeled by fluorescence agents for imaging, the separated oil phase can dissolve anticancer lipophilic molecules (5-fluorouracil, paclitaxel or doxorubicin) for therapeutic application, and the interested proteins are encapsulated to the inner aqueous phase to provide a potential way for protein delivery. Compared with the previously reported BSA capsules in nanosize or microsize, [18][19][20][21] this novel fluorescent-labeled, biodegradable, and protein-lipidpolymer nanocapsule presented typical w/o/w features that could be used for the co-delivery of lipophilic and protein drugs for theranostics.…”

Section: Introductionmentioning

confidence: 90%

Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging

Qian

Zhang

et al. 2015

IJN

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Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein–lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

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“…Moreover, the presence of humic acids inhibits the generation of intracellular reactive oxygen species (ROS) and cellular lipid peroxidation which could be responsible for the lower toxicity of nanoparticles coated with natural organic matter (Lin et al, 2012;Yin et al, 2010). In contrast, BSA is commonly used to increase the bioavailability of NPs in in vivo or in vitro drug delivery (Kim et al, 2009). While BSA increases the colloidal stability of nanoparticles, it also increases the bioavailability of nano-Se c , thereby making the particles to be more toxic.…”

Section: Effect Of Eps On Nano-se Toxicitymentioning

confidence: 99%