Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging

Abstract

Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein–lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

Immune cell impact of three differently coated lipid nanocapsules: pluronic, chitosan and polyethylene glycol

“…2B ). This data gives further proof of the great potential of NCs to perform intracellular drug delivery 3 6 33 43 44 45 . The impact of the differently functionalized NCs on cell viability was studied performing a live/dead staining.…”

Mitotherapy for Fatty Liver by Intravenous Administration of Exogenous Mitochondria in Male Mice

“…Saline solution of the GFP-tagged mitochondria (0.2 ml) and GPP control (stored in our laboratory) (Qian et al, 2015), were respectively, injected slowly via tail veins into mice at a dose of 0.5 mg/kg body weight. Two hours later, the mice were deeply anesthetized with 4% chloral hydrate and transcardically perfused with PBS (0.01 M, pH 7.4) to remove blood.…”

Designing Natural Polymer-Based Capsules and Spheres for Biomedical Applications—A Review

“…A solid core of various natural [11] as well as synthetic polymers [24], metallic particles [23], and composites, have been prepared to make up a hydrophilic or a hydrophobic core, depending upon the type of moieties present in the precursor materials and application-based requirements. Liquid cores of organic solvent [22], oils [16], and a variety of aqueous media [25] have also been prepared to disperse either hydrophobic or hydrophilic APCs. In addition, hollow/porous capsules, made of natural polymers, have also been developed [18,26].…”

“…The APC can be dissolved or dispersed in either the core or the shell matrix (Figure 2). In the case of liquid-core capsules, the APC can either be dissolved in an oily carrier [12] or exist as an aqueous core [25]. Alternatively, it can also be encapsulated by the polymeric shell in its free form.…”

“…Qian et al prepared protein-lipid nanocapsules of fluorescently tagged-BSA (FITC-BSA) shells with double emulsion features, wherein oily shell containing PLGA-linolic acid encapsulated a protein-containing aqueous core for the co-delivery of lipophilic paclitaxel and hydrophilic transcription factor p53 for cancer theragnostic [25]. Prepared by the double emulsion technique, the obtained nanocapsules were ~180 nm in diameter, with a zeta potential of −36.4 mV.…”