Fifty staphylococcal strains that produced toxic shock syndrome (TSS) toxin 1 and that were isolated from patients with TSS were characterized. One strain had more properties that were characteristic of Staphylococcus hyicus than of Staphylococcus aureus. Forty-four strains had the same properties or differed in only one property. Thirty-five of the 50 strains produced either enterotoxin A or C or both in addition to TSS toxin 1. Staphylococcal strains isolated from patients with toxic shock syndrome (TSS) have been classified as Staphylococcus aureus but have been only partially characterized otherwise, primarily for hemolysin production (5, 7, 8), their reaction to phages (2, 22),.and their reaction to antibiotics (23). Todd et al. (23) examined 20 TSS strains for several other properties but did not give specifics about some of the properties, such as coagulase and DNase production, mannitol fermentation, and hemolysis. Only 80% of their TSS strains produced TSS toxin 1 (TSST-1), the toxin accepted as the most likely cause of TSS (M. S. Bergdoll and P. M. Schlievert, Letter, Lancet ii:691, 1984). The 50 TSS strains used in the study reported here were selected on the basis of production of TSST-1 to determine the uniformity of their characteristics. The characteristics examined were as follows: catalase production; aerobic utilization of glucose, mannitol, sucrose, and trehalose (3); anaerobic utilization of glucose and mannitol (21); lysostaphin sensitivity (25); enterotoxin production (18); coagulase production with rabbit, human, and bovine plasma (20); thermonuclease (TNase) production (15); acetoin production