Staphylococcal Gastroenteritis

Jay, James M.

doi:10.1007/978-94-011-6480-1_19

Cited by 18 publications

(4 citation statements)

References 97 publications

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“…Previous reports confirmed the enterotoxigenicity of other Staphylococcus spp. other than S. aureus (Jay, 1992). In this study, S. warneri JRT4 harboured staphylococcal enterotoxins sea genes and biochemically was positive to coagulase.…”

Section: Discussionmentioning

confidence: 47%

Occurrence of virulent and antibiotic resistant Staphylococcus species in ready-to-eat Rhynchophorus phoenicis and Archachatina marginata vended along the Port Harcourt-Bayelsa route

Ngoka¹,

Eruteya²,

Ibiene³

2021

Afr. J. Microbiol. Res.

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This study was aimed at determining the occurrence of Staphylococcus species in ready-to-eat (RTE) Rhynchophorus phoenicis (edible larvae) and Archachatina marginata (land snail) vended along Port Harcourt-Bayelsa route. Eighty samples from four locations were analysed employing standard techniques for proximate and microbiological analyses; virulence determination and antibiotic susceptibility. Data were statistically analyzed using ANOVA and t-test. The mean proximate results revealed the presence of protein (26.01/13.6%), lipid (18.9/3.88%), fibre (5.12/2.01%), ash (3.40/1.11%), moisture (13.47/59.5%) and carbohydrate (32.43/20.07%) for edible larvae/snail, respectively. Of the 80 samples examined, 33 (41.25%) and 52 (65.00%) had total viable bacterial counts and total staphylococcal counts above acceptable microbiological limits, respectively for RTE foods. Seven of the 81 characterized Staphylococcus produced the expected band of 950 bp with sea virulent genes while three produced expected bands of 550 bp with seb virulent genes. Three Staphylococcus aureus strains from edible larvae harboured both virulent genes. The virulent genes bearing Staphylococcus were 100% resistant to augmentin, ceftazidime and cloxacillin but showed varying resistance against ceftriaxone (57.14%), cefuroxime (28.57%), vancomycin (42.86%), oxacillin (42.86%) and cefoxitin (42.86%). The study showed that these RTE foods are potential sources of staphylococcal food poisoning in commuters; hence, food vendors need to conform to standard practice.

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Section: Discussionmentioning

confidence: 47%

Occurrence of virulent and antibiotic resistant Staphylococcus species in ready-to-eat Rhynchophorus phoenicis and Archachatina marginata vended along the Port Harcourt-Bayelsa route

Ngoka¹,

Eruteya²,

Ibiene³

2021

Afr. J. Microbiol. Res.

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“…It has been found widespread in nature and also in warm‐blooded animals, including humans, the skin and nasal cavity being the main human reservoirs of Staph. aureus (Jay 1986). Food handlers and asymptomatic carriers are often sources of food contamination and so the presence of this bacterium or their enterotoxins in foods is usually indicative of lack of hygiene in the food production.…”

Section: Introductionmentioning

confidence: 99%

PCR-based procedures for detection and quantification of Staphylococcus aureus and their application in food.

2006

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Aims: To evaluate the specificity of nuc targeted primers for PCR detection of Staphylococcus aureus in different food matrices and to establish a RTQ‐PCR procedure suitable for the routine detection and quantification of this pathogen in food. Methods and Results: Specificity of nuc targeted primers (Pri1–Pri2 and the newly designed RTQ‐PCR primers) was tested on a total of 157 strains of genetically confirmed identity, including reference and food isolates. PCR detection on artificially inoculated beef samples by DNA extraction using a DNeasy Tissue Kit (Qiagen GmhH, Hilden, Germany) showed a sensitivity value around 103 CFU g−1. The two RTQ‐PCR systems, incorporating SYBR‐Green I and TaqMan, respectively, applied in the present work improved the sensitivity of conventional PCR by lowering the detection level to 10 and 100 cells, respectively. Out of 164 naturally contaminated foods tested for the presence of Staph. aureus, 74 were positive by conventional PCR and 69 by the traditional culture method with a high degree of result agreement between both methodologies (93·3%). Conclusions: PCR approaches, using nuc targeted primers, have proved specific and combined with growth techniques may improve detection of Staph. aureus in different types of food. Significance and Impact of the Study: The SYBR‐Green I real‐time PCR approach established allows the sensitive, automated and quantitative detection of Staph. aureus for routine analysis at a reasonable cost.

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“…Bacteria from the genus Macrococcus belong to the Staphylococcaceae family and may originate from the abattoir workers or the hides of animals. It is believed to be an important contaminant in processed meats (Jay et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Assessment of bacterial superficial contamination in classical or ritually slaughtered cattle using metagenetics and microbiological analysis

Korsak

Taminiau

Hupperts

et al. 2017

International Journal of Food Microbiology

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The aim of this study was to investigate the influence of the slaughter technique (Halal vs Classical slaughter) on the superficial contamination of cattle carcasses, by using traditional microbiological procedures and 16S rDNA metagenetics. The purpose was also to investigate the neck area to identify bacteria originating from the digestive or the respiratory tract. Twenty bovine carcasses (10 from each group) were swabbed at the slaughterhouse, where both slaughtering methods are practiced. Two swabbing areas were chosen: one "legal" zone of 1600 cm 2 (composed of zones from rump, flank, brisket and forelimb) and locally on the neck area (200 cm 2 ). Samples were submitted to classical microbiology for aerobic Total Viable Counts (TVC) at 30°C and Enterobacteriaceae counts, while metagenetic analysis was performed on the same samples. The classical microbiological results revealed no significant differences between both slaughtering practices; with values between 3.95 and 4.87 log CFU/100 cm 2 and 0.49 and 1.94 log CFU/100 cm 2 , for TVC and Enterobacteriaceae respectively. Analysis of pyrosequencing data showed that differences in the bacterial population abundance between slaughtering methods were mainly observed in the "legal" swabbing zone compared to the neck area. Bacterial genera belonging to the Actinobacteria phylum were more abundant in the "legal" swabbing zone in "Halal" samples, while Brevibacterium and Corynebacterium were encountered more in "Halal" samples, in all swabbing areas. This was also the case for Firmicutes bacterial populations (families of Aerococcaceae, Planococcaceae). Except for Planococcoceae, the analysis of Operational Taxonomic Unit (OTU) abundances of bacteria from the digestive or respiratory tract revealed no differences between groups. In conclusion, the slaughtering method does not influence the superficial microbiological pattern in terms of specific microbiological markers of the digestive or respiratory tract. However, precise analysis of taxonomy at the genus level taxonomy highlights differences between swabbing areas. Although not clearly proven in this study, differences in hygiene practices used during both slaughtering protocols could explain the differences in contamination between carcasses from both slaughtering groups.

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Staphylococcal Gastroenteritis

Cited by 18 publications

References 97 publications

Occurrence of virulent and antibiotic resistant Staphylococcus species in ready-to-eat Rhynchophorus phoenicis and Archachatina marginata vended along the Port Harcourt-Bayelsa route

Occurrence of virulent and antibiotic resistant Staphylococcus species in ready-to-eat Rhynchophorus phoenicis and Archachatina marginata vended along the Port Harcourt-Bayelsa route

PCR-based procedures for detection and quantification of Staphylococcus aureus and their application in food.

Assessment of bacterial superficial contamination in classical or ritually slaughtered cattle using metagenetics and microbiological analysis

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