Brucellosis

Renner, Edward D.

doi:10.1016/s0196-4399(79)80075-6

Cited by 7 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The laboratory diagnosis of brucellosis is made primarily by serological tests because the organism is isolated by cultural methods in no more than 20% of the cases (14). The routine brucella agglutination test is the most frequently used type of serological test for this purpose; however, it does not differentiate between active and inactive disease because it does not differentiate between between IgG and IgM agglutinins.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Brucella antibodies in bovine sera by rose bengal plate test (RBPT), serum agglutination test (SAT), microagglutination test (MAT) and 2-mercaptoethanol-microagglutination (2-ME-MAT)

Sareyyüpoğlu¹,

Cantekin²,

Müştak³

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Summary:In this study, for the investigation of Brucella antibodies a total number of 524 serum samples were sent to the Department of Microbiology, Faculty of Veterinary Medicine, Ankara University. For the determination of most appropriate test for the investigation, first 100 samples were investigated by rose bengal plate test (RBPT), serum agglutination test (SAT), microagglutination test (MAT) and 2-mercaptoethanol-microagglutination test (2-ME-MAT). Following this pre-investigation study, 3 (3%) and 5 (5%) samples were found to be positive with RBPT and SAT, MAT, 2-ME-MAT (same results were achieved with each of the three tests) tests, respectively. No statistically significant difference was observed amongst the later three tests (p>0.05). Because it has a short evaluation time and since it was found to be economical concerning the used expenditure and reagents, MAT was preferred as the most appropriate test for the serological investigation. Following MAT tests of 524 serum samples 8 (1.5%) samples were found to be positive concerning Brucella antibodies. As a conclusion, MAT was determined to be a fast, reliable and an economic test, which can be easily used in routine diagnostic laboratories for serological diagnosis.Key words: Brucella, microagglutination, serology, serum agglutination Sığır serum örneklerinde Brucella antikorlarının belirlenmesinde rose bengal plate test (RBPT), serum aglutinasyon test (SAT), mikroaglutinasyon test (MAT) ve 2-merkaptoetanolmikroagglutinasyon (2-ME-MAT) testlerinin karşılaştırılmasıÖzet: Bu çalışmada, özel bir sığırcılık işletmesinden Ankara Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalı'na gönderilen toplam 524 serum örneğinin ilk 100 örneğinde Brucella antikorlarının belirlenmesi amacıyla, Rose Bengal Plate Test (RBPT), Serum Aglutinasyon Test (SAT), Mikroaglutinasyon Test (MAT) ve 2-merkaptoetanol-Mikroagglutinasyon (2-ME-MAT) Testi uygulandı. Yapılan ön çalışma sonucunda RBPT ile 3 (% 3), SAT, MAT ve 2-ME-MAT ile 5 (% 5) örnekte pozitiflik saptandı. Son üç test ile elde edilen titreler arasında istatistiksel bir farklılığın olmadığı (p>0.05) belirlendi. Kullanılan sarf malzemesi ve reaktifler açısından ekonomik olması ve değerlendirme süresinin kısa olması nedeniyle örneklerin değerlendirilmesinde MAT testi tercih edildi. MAT testi ile 524 örneğin incelenmesi sonucunda 8 (% 1.5) örnekte pozitiflik saptandı. Sonuç olarak, MAT'ın serolojik teşhis yapan rutin laboratuvarlarında hızlı, kolay, güvenilir ve ekonomik bir test olduğu belirlendi. Anahtar sözcükler: Brucella, mikroaglutinasyon, seroloji, serum agglutinasyon.

show abstract

Section: Introductionmentioning

confidence: 99%

Investigation of Brucella antibodies in bovine sera by rose bengal plate test (RBPT), serum agglutination test (SAT), microagglutination test (MAT) and 2-mercaptoethanol-microagglutination (2-ME-MAT)

Sareyyüpoğlu¹,

Cantekin²,

Müştak³

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

show abstract

“…In contrast to the procedure of Renner & McMahon (1981), however, 18 h incubation was performed at 50°C instead of 37°C to reduce the number of non-specific reactions (Wilson & Miles, 1975). The control serum titer then amounted to 160 rather than the titre of 320 indicated by the Institute of Veterinary Medicine.…”

mentioning

confidence: 99%

Brucellosis: serological methods compared

Heizmann

Botzenhart

Döller

et al. 1985

J. Hyg.

View full text Add to dashboard Cite

SUMMARYAt least 12 persons contracted clinical, and 4 persons subelinical Brucella melitensis infection during a brucellosis epidemic in the spring and summer of 1983 in Southern Germany, a region which had been free of this disease for the past 20 years. All cases of illness were traced to one infected herd of sheep. The presence of antibodies against B. melitensis was examined in 72 sera of infected patients using the following tests: agglutination, Coomb's test, two complement fixation tests with different antigen preparations (CFT 1 and 2), IgG and IgM enzyme-linked immunosorbent assay (ELISA), and opsonophagocytosis; and the occurrence of cross-reacting antibodies against Yersinia enterocolitica 0 9 was investigated in the agglutination and complement fixation tests. Sera from 100 blood donors and 112 other people with close contact with sheep were also examined. The results revealed the need to consider an intermediate range in the interpretation of ELISA results -due to elevated values of persons in groups at risk but without clinical signs of illness. In all other tests, however, such persons revealed the same cut-off levels as the general population. Results from all initial sera of infected persons revealed titres of optical densities above the baseline levels determined in the present study, with the exception ofthe Coomb's and CFT 2 tests. The agglutination test, but not brucella CFT2, revealed complete cross-reactivity between Y. enterocolitica 09 and B. melitensis. ELISA stood out as the only test which is suited to diagnosis of both recent and past infection, since ELISA IgM determination permits conclusions about the time of the onset of illness, and determination of IgG may still yield values above the cut-off level up to 623 days after the onset of illness. In 2 of the 16 infected persons, IgG ELISA was the only test revealing previous infection 424 and 528 days after the onset of illness. A procedural scheme is presented which may help to simplify the diagnosis of brucellosis.

show abstract

“…To evaluate the technique described, we reviewed the TAT results in the first serum sample from 73 random cases in which brucellosis was confirmed by blood culture and carried out CM on the 16 sera (22%) showing TAT titers of <160, generally accepted as a borderline dilution below which diagnosis is not conclusive (5). The results are shown in Table 1.…”

mentioning

confidence: 99%