Brucella papionis sp. nov., isolated from baboons (Papio spp.)

Abstract

Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60 T and F8/ 08-61) isolated from clinical specimens obtained from baboons (Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus Brucella. This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate. All of the above analyses suggested that the two strains represent a novel lineage within the genus Brucella. The strains also possessed a unique profile when subjected to the phenotyping approach classically used to separate species of the genus Brucella, reacting Abbreviations: MLSA, multilocus sequence analysis; MLVA, multilocus variable number of tandem repeat analysis; RTD, routine test dilution.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, omp2a and omp2b gene sequences of strains F8/08-60 T and F8/08-61 are HG932316 and HG932317 (16S rRNA gene), KJ493822 and KJ493823 (omp2a) and KJ510540 and KJ510541 (omp2b), respectively.A supplementary table and a supplementary figure are available with the online Supplementary Material.

Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes)

“…Because of their high metabolic activity, B. microti and B. inopinata are commonly misidentified as Ochrobactrum in commercially available biochemical test systems such as API 20NE (bioMérieux). The most recently described species, B. papionis, was isolated from a primate stillbirth (Whatmore et al, 2014). B. papionis is genetically most closely related to B. ovis, and growth is comparable to that of classical species of Brucella.…”

“…The observed phage plaques Table 1. Metabolic activity of the novel species in comparison with classical and recently described species tested with the Micronaut BfR Brucella assay Taxa: 1, B. abortus; 2, B. melitensis; 3, B. suis; 4, B. ovis; 5, B. canis; 6, B. neotomae; 7, B. ceti; 8, B. pinnipedialis (Al ; 9, B. microti (Scholz et al, 2008a); 10, B. inopinata ; 11, B. papionis (Whatmore et al, 2014); 12, B. vulpis sp. nov. 2, No metabolic activity; +, metabolic activity; V, variable metabolic activity.…”

Brucelosis: zoonosis de importancia en México

“…Sin embargo, países como Canadá, E.U.A., Japón, el norte y centro de Europa, Australia y Nueva Zelandia han logrado erradicar esta enfermedad 8,9 . 15 . Recientemente, a partir de muestras de nódulos linfáticos de zorros rojos en Austria, se aisló una nueva especie la que fue descrita como: B. vulpis 16 .…”

Comparisons of brucellosis between human and veterinary medicine

“…Zoonotic potential is classified as pathogenicity and virulence in human hosts. Original citation indicates the original publication where the species was characterized.SpeciesNatural hostZoonotic Potential [8]Original Citation B. melitensis Sheep, goats, and camelsYes – High[5] B. abortus Cattle, elk, and bisonYes – High[6] B. suis Pigs, hare, reindeer/caribouYes – High[122] B. canis Dogs (domestic and wild)Yes – Moderate[123] B. ovis SheepNo reported infections[124] B. neotomae Desert wood ratsNo reported infections[125] B. ceti CetaceansYes – Low[126] B. pinnipedialis PinnipedsYes – Low B. microti Red foxes and common volesNo reported infections[127] B. inopinata UnknownYes – High[2,128] B. papionis Non-Human PrimatesNo reported infections[129,130] B. vulpis Red foxNo reported infections[131,132] Brucella NFXXXX Australian ratNo reported infections[133,134]…”