Brucella abortus efp gene is required for an efficient internalization in HeLa cells

Iannino, Florencia; Ugalde, Juan Esteban; Iannino, Nora Iñón de

doi:10.1016/j.micpath.2011.09.008

Cited by 17 publications

(19 citation statements)

References 34 publications

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“…Subsequently, similar results observed for efp , poxA , and yjeK mutants of Salmonella enterica led to a better description of the coordinated effects of these genes in virulence and stress resistance . EF‐P has also been shown to be involved in cell motility and susceptibility to hyperosmotic conditions in many bacteria . As suggested for eIF5A, these data led to the hypothesis that EF‐P could be involved in the specialized translation of mRNAs.…”

Section: Eif5a Is Now a Translation Elongation Factor As Its Bacterimentioning

confidence: 58%

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eIF5A and EF‐P: two unique translation factors are now traveling the same road

et al. 2014

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Translational control is extremely important in all organisms, and some of its aspects are highly conserved among all primary kingdoms, such as those related to the translation elongation step. The previously classified translation initiation factor 5A (eIF5A) and its bacterial homologue elongation factor P (EF-P) were discovered in the late 70's and have recently been the object of many studies. eIF5A and EF-P are the only cellular proteins that undergo hypusination and lysinylation, respectively, both of which are unique posttranslational modifications. Herein, we review all the important discoveries related to the biochemical and functional characterization of these factors, highlighting the implication of eIF5A in translation elongation instead of initiation. The findings that eIF5A and EF-P are important for specific cellular processes and play a role in the relief of ribosome stalling caused by specific amino acid sequences, such as those containing prolines reinforce the hypothesis that these factors are involved in specialized translation. Although there are some divergences between these unique factors, recent studies have clarified that they act similarly during protein synthesis. Further studies may reveal their precise mechanism of ribosome activity modulation as well as the mRNA targets that require eIF5A and EF-P for their proper translation.

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Section: Eif5a Is Now a Translation Elongation Factor As Its Bacterimentioning

confidence: 58%

“…36 EF-P has also been shown to be involved in cell motility and susceptibility to hyperosmotic conditions in many bacteria. 78,83,[85][86][87] As suggested for eIF5A, these data led to the hypothesis that EF-P could be involved in the specialized translation of mRNAs.…”

Section: Eif5a Is Now a Translation Elongation Factor As Its Bacterimentioning

confidence: 81%

eIF5A and EF‐P: two unique translation factors are now traveling the same road

et al. 2014

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“…This locus encodes for a glycerol-3phosphate binding ABC transporter present in the outer membrane of pathogenic and nonpathogenic bacteria. The B. abortus efp gene was also shown to have a defect in internalization but no attenuation in macrophages or in vivo (Iannino et al, 2012). Additional recent studies have implicated other Brucella proteins in adhesion/internalization into nonprofessional phagocytes.…”

Section: Brucella Intracellular Traffickingmentioning

confidence: 98%

Internal affairs: investigating theBrucellaintracellular lifestyle

2012

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Bacteria of the genus Brucella are Gram‐negative pathogens of several animal species that cause a zoonotic disease in humans known as brucellosis or Malta fever. Within their hosts, brucellae reside within different cell types where they establish a replicative niche and remain protected from the immune response. The aim of this article is to discuss recent advances in the field in the specific context of the Brucella intracellular ‘lifestyle’. We initially discuss the different host cell targets and their relevance during infection. As it represents the key to intracellular replication, the focus is then set on the maturation of the Brucella phagosome, with particular emphasis on the Brucella factors that are directly implicated in intracellular trafficking and modulation of host cell signalling pathways. Recent data on the role of the type IV secretion system are discussed, novel effector molecules identified and how some of them impact on trafficking events. Current knowledge on Brucella gene regulation and control of host cell death are summarized, as they directly affect intracellular persistence. Understanding how Brucella molecules interplay with their host cell targets to modulate cellular functions and establish the intracellular niche will help unravel how this pathogen causes disease.

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“…Therefore, stress conditions that require expression of large numbers of polyproline-containing proteins will require the presence of active EF-P or a/eIF-5A. The absence of efp, yjeK, or yjeA genes has been shown to cause defects in growth (Yanagisawa et al, 2010;Iannino et al, 2012;Zou et al, 2012), fitness (de Crecy et al, 2007), membrane integrity (Iannino et al, 2012;Zou et al, 2012), stress response (Ude et al, 2013), sporulation (Ohashi et al, 2003), motility (Kearns et al, 2004;Inoue et al, 2007;Zou et al, 2012), antibiotics sensitivity, colonization, and virulence (Kaniga et al, 1998;Peng et al, 2001;Merrell et al, 2002;Bearson et al, 2006Bearson et al, , 2011Navarre et al, 2010;Iannino et al, 2012) in a variety of bacteria.…”

Section: Introductionmentioning

confidence: 99%

The bacterial translation stress response

et al. 2014

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Throughout their life, bacteria need to sense and respond to environmental stress. Thus, such stress responses can require dramatic cellular reprogramming, both at the transcriptional as well as the translational level. This review focuses on the protein factors that interact with the bacterial translational apparatus in order to respond to and cope with different types of environmental stress. For example, the stringent factor RelA interacts with the ribosome to generate ppGpp under nutrient deprivation, whereas a variety of factors have been identified that bind to the ribosome under unfavorable growth conditions to shut-down (RelE, pY, RMF, HPF and EttA) or re-program (MazF, EF4 and BipA) translation. Additional factors have been identified that rescue ribosomes stalled due to stress-induced mRNA truncation (tmRNA, ArfA, ArfB), translation of unfavorable protein sequences (EF-P), heat shock induced subunit dissociation (Hsp15) or antibiotic inhibition (TetM, FusB). Understanding the mechanism of how the bacterial cell responds to stress will not only provide fundamental insight into translation regulation, but will also be an important step to identifying new targets for the development of novel antimicrobial agents.

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Brucella abortus efp gene is required for an efficient internalization in HeLa cells

Cited by 17 publications

References 34 publications

eIF5A and EF‐P: two unique translation factors are now traveling the same road

eIF5A and EF‐P: two unique translation factors are now traveling the same road

Internal affairs: investigating theBrucellaintracellular lifestyle

The bacterial translation stress response

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