Bruce/apollon promotes hippocampal neuron survival and is downregulated by kainic acid

Endoplasmic Reticulum Stress Inhibition Protects against Excitotoxic Neuronal Injury in the Rat Brain

“…The mode of cell death caused by KA includes activation of caspases as well as changes in expression of various pro-and anti-apoptotic molecules (Korhonen et al, 2001(Korhonen et al, , 2003Sokka et al, 2005). Members of the Bcl-2 and IAP (inhibitor of apoptosis protein) families have been shown to modulate the KA-mediated cell death (Lopez et al, 1999;Korhonen et al, 2003;Sokka et al, 2005).…”

“…Hippocampal neurons prepared from embryonic day 17 rats (Harlan, Horst, The Netherlands) were cultured for 7 d on poly-ornithine (Sigma)-coated dishes in Neurobasal medium with B27 supplement (Invitrogen, Espoo, Finland) as described previously (Korhonen et al, 2001(Korhonen et al, , 2003Sokka et al, 2005). Different concentrations of KA (Calbiochem) or glutamate (Sigma) were added to the cells for various periods of time.…”

Endoplasmic Reticulum Stress, Inflammation, and Perinatal Brain Damage

“…On the other hand, another excitatory transmitter, kainic acid (KA), activates ER sensors that are part of the PERK, ATF6, and IRE1 pathways (66). The mode of cell death caused by KA includes the activation of caspases as well as changes in expression of various pro- and anti-apoptotic molecules (67). KA induces fragmentation of the ER membrane in neurons, which in turn activates the ER proteins Bip and CHOP.…”

HILAQ: A Novel Strategy for Newly Synthesized Protein Quantification

“…For example, BIRC6 (Baculoviral IAP repeat-containing protein 6) is part of a protein family that inhibits cell death through the inactivation of caspases and was up-regulated in HT22 cells. It has been reported that the hippocampus down-regulated this protein in response to glutamate toxicity(29). In order for cell death to proceed, cells degrade BIRC6 through the proteasome (30).…”