Bronchodilator effects of Lignosus rhinocerotis  extract on rat isolated airways is linked to the blockage of calcium entry

Lee, Mei-Kee; Lim, Kuan‐Hon; Millns, Paul; Mohankumar, Suresh Kumar; Ng, Szu-Ting; Tan, Cher Siang; Then, Sue Mian; Mbaki, Yvonne; Ting, Kang Nee

doi:10.1016/j.phymed.2018.03.025

Cited by 12 publications

(11 citation statements)

References 25 publications

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“…The effectiveness of the HMW fraction in blocking the calcium-induced contraction was similar to nifedipine thus implying the HMW fraction may be blocking voltage-gated Ca 2+ channels. This is supported by observations from an earlier calcium imaging study showing that CWE (likely HMW fraction as well) inhibits extracellular Ca 2+ influx into rat dorsal root ganglion cells ( Lee et al, 2018 ). Given this evidence, we conclude that one of the mechanisms by which the CWE induces airway relaxation is linked to regulation of calcium signalling in response to G αq -protein-coupled receptor activation.…”

Section: Discussionsupporting

confidence: 74%

“…Two most common bronchodilators used to reverse airways constriction act via stimulation of β 2 -adrenoceptors (e.g., salmeterol) or antagonism of muscarinic receptors (e.g., ipratropium). The relaxation effects of CWE are not mediated through β 2 -adrenoceptor stimulation, nitric oxide, or K + channel (see Supplementary Figure 1 and Lee et al, 2018 ) or blockade of muscarinic receptors (see Figure 3A ). Our evidence to date implies that CWE relaxes airways through a mechanism different from that used by the conventional bronchodilators.…”

Section: Discussionmentioning

confidence: 98%

“…This is the first study to elucidate the bioactive fractions accounting for the airway relaxation effect elicited by this mushroom. Our earlier study showed that CWE has fully relaxed both the isolated tracheal and bronchial segments pre-contracted with carbachol ( Lee et al, 2018 ). Two most common bronchodilators used to reverse airways constriction act via stimulation of β 2 -adrenoceptors (e.g., salmeterol) or antagonism of muscarinic receptors (e.g., ipratropium).…”

Section: Discussionmentioning

confidence: 99%

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Airway Relaxation Effects of Water-Soluble Sclerotial Extract From Lignosus rhinocerotis

Lee

Yap

et al. 2018

Front. Pharmacol.

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Lignosus rhinocerotis has a long history of use by the indigenous community within East Asia to treat a range of health conditions including asthma and chronic cough. To date, there is limited scientific evidence to support its therapeutic effects in relieving these airways conditions. In this study, we examined the effects of the different molecular weight fractions [high-molecular-weight (HMW), medium-molecular-weight (MMW), and low-molecular-weight (LMW)] obtained from the cold water sclerotial extract (CWE) of L. rhinocerotis on airways patency using airway segments isolated from Sprague Dawley rat in an organ bath set-up. It is demonstrated that the HMW and MMW fractions exhibited higher efficacy in relaxing the pre-contracted airways when compared to the CWE and LMW fraction. In addition, the HMW fraction markedly supressed carbachol-, 5-hydroxytrptamine-, and calcium-induced airway contractions. CWE demonstrated a lower efficacy than the HMW fraction but it also significantly attenuated carbachol- and calcium-induced airway contractions. Results showed that the bronchorelaxation effect of CWE and fractions is mediated via blockade of extracellular Ca2+ influx. The composition analysis revealed the following parts of carbohydrate and proteins, respectively: HMW fraction: 71 and 4%; MMW fraction: 35 and 1%; and LMW fraction: 22 and 0.3%. Our results strongly suggest that the polysaccharide–protein complex or proteins found in the HMW and MMW fractions is likely to contribute to the bronchorelaxation effect of CWE.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Airway Relaxation Effects of Water-Soluble Sclerotial Extract From Lignosus rhinocerotis

Lee

Yap

et al. 2018

Front. Pharmacol.

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“…(Selangor, Malaysia). The preparation method L. rhinocerotis CWE is reported in our previous paper [1] . The percentage yield of the CWE was 10% (w/w).…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“… Experiments were carried out at the University of Nottingham, UK. Data accessibility The data is presented in this article Related research article Bronchodilator effects of Lignosus rhinocerotis extract on rat isolated airways is linked to the blockage of calcium entry [1] …”

mentioning

confidence: 99%

Data on the Lignosus rhinocerotis water soluble sclerotial extract affecting intracellular calcium level in rat dorsal root ganglion cells

et al. 2018

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The data in this article contain supporting evidence for the research manuscript entitled “Bronchodilator effects of Lignosus rhinocerotis extract on rat isolated airways is linked to the blockage of calcium entry” by Lee et al. (2018) [1]. The data were obtained by calcium imaging technique with fluorescent calcium indicator dyes, Fura 2-AM, to visualize calcium ion movement in the rat dorsal ganglion (DRG) cells. The effects of L. rhinocerotis cold water extract (CWE1) on intracellular calcium levels in the DRG cells were presented.

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Folium Sennae and emodin reverse airway smooth muscle contraction

Qiu¹,

Ma²,

Liu³

et al. 2020

Cell Biology International

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The objective of this project was to find a bronchodilatory compound from herbs and clarify the mechanism. We found that the ethanol extract of Folium Sennae (EEFS) can relax airway smooth muscle (ASM). EEFS inhibited ASM contraction, induced by acetylcholine, in mouse tracheal rings and lung slices. High‐performance liquid chromatography assay showed that EEFS contained emodin. Emodin had a similar reversal action. Acetylcholine‐evoked contraction was also partially reduced by nifedipine (a selective inhibitor of L‐type voltage‐dependent Ca2+ channels, LVDCCs), YM‐58483 (a selective inhibitor of store‐operated Ca2+ entry, SOCE), as well as Y‐27632 (an inhibitor of Rho‐associated protein kinase). In addition, LVDCC‐ and SOCE‐mediated currents and cytosolic Ca2+ elevations were inhibited by emodin. Emodin reversed acetylcholine‐caused increases in phosphorylation of myosin phosphatase target subunit 1. Furthermore, emodin, in vivo, inhibited acetylcholine‐induced respiratory system resistance in mice. These results indicate that EEFS‐induced relaxation results from emodin inhibiting LVDCC, SOCE, and Ca2+ sensitization. These findings suggest that Folium Sennae and emodin may be new sources of bronchodilators.

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Bronchodilator effects of Lignosus rhinocerotis extract on rat isolated airways is linked to the blockage of calcium entry

Cited by 12 publications

References 25 publications

Airway Relaxation Effects of Water-Soluble Sclerotial Extract From Lignosus rhinocerotis

Airway Relaxation Effects of Water-Soluble Sclerotial Extract From Lignosus rhinocerotis

Data on the Lignosus rhinocerotis water soluble sclerotial extract affecting intracellular calcium level in rat dorsal root ganglion cells

Folium Sennae and emodin reverse airway smooth muscle contraction

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