Bronchoconstriction In Mice Allergic To House Dust Mite: A Role For Mast Cells?

Lundblad, Lennart K. A.; Li, Shannon; Aliyeva, Minara; Daphtary, Nirav; Velden, Jos L.J. van der; Hoffman, Sidra M.; Lahue, Karrie G.; Janssen–Heininger, Yvonne M. W.; Poynter, Matthew E.; Bates, Jason H. T.; Stevenson, Christopher S.; Phillips, Jonathan E.

doi:10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a2405

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 In order to interpret impedance measurements, we need to be able to correlate the parameters with tangible and quantifiable properties of the respiratory system. This has been performed extensively in animal models 16,103,104 and now, more recently, in humans with lung disease. Using positron emission tomography (PET), it was demonstrated that patients with obstructive lung disease have distorted ventilation patterns that are distinctly different from that of a healthy lung, which can lead to cluster formation that eventually can lead to catastrophic shifts in lung function.…”

Section: Copd With Emphysemamentioning

confidence: 99%

Applications of oscillometry in clinical research and practice

Lundblad

Siddiqui

Bossé

et al. 2019

Canadian Journal of Respiratory, Critical Care, and Sleep Medic

View full text Add to dashboard Cite

Oscillometry is gaining in clinical use and while there is an increased interest in the technique, there is paucity in the understanding of its possibilities and limitations. Oscillometry has seen extensive use in research over several decades, but only recently is the technique being adopted in clinical practice; hence, there is a need to educate the novel users. The goal of the mini symposium arranged in San Diego in 2018 was to discuss the principles of oscillometry, showcase some of the recent ongoing research using this technique and to demonstrate how oscillometry may be used in clinical practice. It was concluded that oscillometry has several advantages over spirometry, most notably, with novel data being shown, its sensitivity allowing early detection of small airways disease not possible with spirometry and it can be used in subjects who have difficulties performing forced maneuvers such as preschool children, the elderly and subjects with handicaps. The site of respiratory pathology can be reflected by the various parameters generated by oscillometry and thus help with both disease diagnosis and localization. While the interpretation of oscillometry parameters and translating them into meaningful pathological correlates is still evolving, it is likely that oscillometry will soon be at the forefront of both pulmonary clinical practice and research. R ESUM EL'oscillom etrie est de plus en plus utilis ee en contexte clinique, mais malgr e l'int erêt accru pour cette technique, la compr ehension de ses possibilit es et de ses limites est encore insuffisante. Pendant plusieurs d ecennies, l'oscillom etrie a largement et e utilis ee dans la recherche, mais l'adoption de cette technique dans la pratique clinique est encore r ecente, de sorte qu'il est n ecessaire de former les nouveaux utilisateurs. Le but de ce mini symposium qui s'est tenu a San Diego en 2018 etait de discuter des principes de l'oscillom etrie, de pr esenter les plus r ecentes etudes utilisant cette technique et de d emontrer comment elle peutêtre utilis ee dans la pratique clinique. Il a et e d etermin e que l'oscillom etrie pr esentait plusieurs avantages par rapport a la spirom etrie notamment, comme le d emontrent de nouvelles donn ees, sa sensibilit e qui permet la d etection pr ecoce de la maladie des petites voies a eriennes, ce que la spirom etrie ne peut pas faire, ainsi que le fait qu'elle puisseêtre utilis ee aupr es de sujets qui eprouvent de la difficult e a effectuer des manoeuvres forc ees comme les enfants d'âge pr escolaire, les personnesâg ees et les personnes atteintes d'un handicap. L'emplacement de la pathologie respiratoire peut se refl eter dans les divers param etres produits par la spirom etrie, ce qui peut contribuer a diagnostiquer la maladie et a la localiser. Bien que l'interpr etation des param etres de l'oscillom etrie et leur traduction dans des corr elats pathologiques significatifs continue d' evoluer, l'oscillom etrie sera vraisemblablement a l'avant-plan de la pratique clinique et de la recherche pulmonair...

show abstract

Section: Copd With Emphysemamentioning

confidence: 99%

Applications of oscillometry in clinical research and practice

Lundblad

Siddiqui

Bossé

et al. 2019

Canadian Journal of Respiratory, Critical Care, and Sleep Medic

View full text Add to dashboard Cite

show abstract

“…Based on preliminary data from our laboratory (29), we hypothesized that HDM sensitization and challenge would induce lung mast cell expansion in mice and that reexposure to inhaled HDM would cause acute bronchoconstriction. We tested this hypothesis by measuring acute bronchoconstriction, the numbers of mast cells in the lung, and the quantities of mediators released from activated mast cells in HDM-sensitized and HDM-challenged wild-type mice and mice deficient in mast cells.…”

mentioning

confidence: 99%

Antigen-induced mast cell expansion and bronchoconstriction in a mouse model of asthma

Aliyeva

Daphtary

et al. 2014

American Journal of Physiology-Lung Cellular and Molecular Phys

Self Cite

View full text Add to dashboard Cite

Lung mastocytosis and antigen-induced bronchoconstriction are common features in allergic asthmatics. It is therefore important that animal models of asthma show similar features of mast cell inflammation and reactivity to inhaled allergen. We hypothesized that house dust mite (HDM) would induce mastocytosis in the lung and that inhalation of HDM would trigger bronchoconstriction. Mice were sensitized with intranasal HDM extract, and the acute response to nebulized HDM or the mast cell degranulating compound 48/80 was measured with respiratory input impedance. Using the constant-phase model we calculated Newtonian resistance (Rn) reflecting the conducting airways, tissue dampening (G), and lung elastance (H). Bronchoalveolar lavage fluid was analyzed for mouse mast cell protease-1 (mMCP-1). Lung tissue was analyzed for cytokines, histamine, and α-smooth muscle actin (α-SMA), and histological slides were stained for mast cells. HDM significantly increased Rn but H and G remained unchanged. HDM significantly expanded mast cells compared with control mice; at the same time mMCP-1, α-SMA, Th2 cytokines, and histamine were significantly increased. Compound 48/80 inhalation caused bronchoconstriction and mMCP-1 elevation similarly to HDM inhalation. Bronchoconstriction was eliminated in mast cell-deficient mice. We found that antigen-induced acute bronchoconstriction has a distinct phenotype in mice. HDM sensitization caused lung mastocytosis, and we conclude that inhalation of HDM caused degranulation of mast cells leading to an acute bronchoconstriction without affecting the lung periphery and that mast cell-derived mediators are responsible for the development of the HDM-induced bronchoconstriction in this model.

show abstract

Bronchoconstriction In Mice Allergic To House Dust Mite: A Role For Mast Cells?

Cited by 2 publications

References 0 publications

Applications of oscillometry in clinical research and practice

Applications of oscillometry in clinical research and practice

Antigen-induced mast cell expansion and bronchoconstriction in a mouse model of asthma

Contact Info

Product

Resources

About