Bromelain protects piglets from diarrhoea caused by oral challenge with K88 positive enterotoxigenic<i>Escherichia coli</i>

Chandler, David; Mynott, Tracey L.

doi:10.1136/gut.43.2.196

Cited by 75 publications

(56 citation statements)

References 14 publications

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“…the CD14 receptor for bacterial LPS on monocytes and the CD21 activation regulator on B cells; 16). Bromelain also removes cell surface receptors that can serve as receptors for intestinal bacteria (16,(37)(38)(39)(40)(41), which may decrease exposure of immune cells to bacterial antigens that stimulate detrimental immune responses in IBD. Proteolysis by bromelain may also affect production of pro-inflammatory cytokines and chemokines by altering concentrations of regulatory cytokines such as IL-10 or TGF-β that was not analyzed in the current study.…”

Section: Discussionmentioning

confidence: 99%

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Onken

Greer

Calingaert

et al. 2008

Clinical Immunology

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Oral bromelain has been anecdotally reported to decrease inflammation in ulcerative colitis (UC). Proteolytically active bromelain is known to decrease expression of mRNAs encoding proinflammatory cytokines by human leukocytes in vitro. To assess the effect of bromelain on mucosal secretion of cytokines in inflammatory bowel disease (IBD), endoscopic colon biopsies from patients with UC, Crohn's disease (CD), and non-IBD controls were treated in vitro with bromelain or media, then cultured. Secretion of pro-inflammatory cytokines and chemokines was measured. Significant increases in granulocyte colony stimulating factor (G-CSF), interferon (IFN)-γ, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) were detected in the media from actively inflamed areas in UC and CD as compared with non-inflamed IBD tissue and non-IBD controls. In vitro bromelain treatment decreased secretion of G-CSF, granulocyte-macrophage colony stimulating factor (GM-CSF), IFN-γ, CCL4/macrophage inhibitory protein (MIP)-1β, and TNF by inflamed tissue in IBD. Bromelain may be a novel therapy for IBD.

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Section: Discussionmentioning

confidence: 99%

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Onken

Greer

Calingaert

et al. 2008

Clinical Immunology

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“…is a rich source of a mixture of cysteine proteinases, the most abundant among them being the fruit bromelain (EC 3.4.22.33) which hydrolytically cleaves the internal peptide bonds in proteins with relatively broad specificity (Rowan and Buttle, 1994). The pineapple proteinases find uses in various industrial and medical applications including brewing, meat tenderization, prevention of diarrhea, digestive aids and treatment of edema (Takagi et al 1992;Tanabe et al 1996;Chandler and Mynott, 1998;Kelly, 1996;Maurer, 2001). The use of enzymes in the silk industry is relatively unexplored, has generated a lot of interest, and much research is being carried out internationally (Gulrajani et al 2000).…”

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confidence: 99%

Enzymological characterization of pineapple extract for potential application in oak tasar (Antheraea proylei J.) silk cocoon cooking and reeling

Singh¹,

Devi²,

Devi³

2003

Electron. J. Biotechnol.

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Proteinases have the potential to effect partial solubilization of the proteinaceous gum sericin involved in binding the silk strands together in cocoon, an essential step in the silk cocoon cooking and reeling. Therefore, pineapple extract rich in cysteine proteinases was enzymologically characterized for its potential application in oak tasar (Antheraea proylei J.) silk cocoon cooking and reeling. Optimum sodium carbonate concentration (9.8 mM) and optimum temperature (60ºC) for the proteinase activity were determined. Though relatively thermostable, an enhanced activity loss was observed when the extract was incubated in the temperature range 70-90ºC with sodium carbonate. Bulk of the activity (80-83%) remained after 1 hr of time-dependent inactivation at 60ºC. The tasar cocoon extract neither caused inhibition of the activity nor enhanced its time-dependent loss by incubation at 60ºC. However, it caused an enhanced time-dependent loss of the activity by incubation at 60ºC *Corresponding author with sodium carbonate. Considering these enzymological characteristics, experimental cocooncooking media were constituted by taking the pineapple extract with or without sodium carbonate at room temperature or 60ºC. The results of the cocoon cooking and subsequent single silk filament reeling indicated for an applicability of pineapple extract as an effective agent for the oak tasar cocoon cooking and reeling.The fruit of pineapple, Ananas comosus (L.) Merr. is a rich source of a mixture of cysteine proteinases, the most abundant among them being the fruit bromelain (EC 3.4.22.33) which hydrolytically cleaves the internal peptide bonds in proteins with relatively broad specificity (Rowan and Buttle, 1994). The pineapple proteinases find uses in various industrial and medical applications including brewing, meat tenderization, prevention of diarrhea, digestive aids and treatment of edema (Takagi et al. 1992;Tanabe et al. 1996;Chandler and Mynott, 1998; Kelly, Singh L.R. et al. 1996;Maurer, 2001). The use of enzymes in the silk industry is relatively unexplored, has generated a lot of interest, and much research is being carried out internationally (Gulrajani et al. 2000). Against this background, pineapple fruit pulp extract (simply pineapple extract) having proteinase activity may also find application in tasar silk industry in general and tasar silk cocoon cooking and reeling in particular. The oak tasar (Antheraea proylei J.) silkworm is an important source of tasar silk (Singh and Singh, 1998). The larvae of the silkworm, reared on leaves of oak tree Quercus species (FamilyFagaceae), produce the cocoons from which the tasar silk is reeled (Singh and Singh, 1998). Unlike the mulberry (Bombyx mori L.) silk cocoons, the oak tasar silk cocoons cannot be satisfactorily softened by boiling in plain water (Jolly et al. 1979). The cocoons containing relatively higher amounts of protein-tannin complexes in the form of proanthocyanidins (Pandey and Goel, 1990;Pandey, 1997), have to be softened by more drastic boiling off tech...

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“…Bromelain is a mixture of cysteine proteases obtained from pineapple stems that protects animals from enterotoxigenic Escherichia coli (ETEC)-induced diarrhea (3,27). Bromelain exerts a dual antidiarrhea action.…”

mentioning

confidence: 99%

“…Bromelain exerts a dual antidiarrhea action. First, it temporarily inactivates enterocyte ETEC receptors such that bacteria cannot colonize the small intestine (3,30). Second, bromelain inhibits intestinal secretion induced by ETEC heat-labile and heatstable toxins by blocking host cell signal transduction downstream from the generation of cyclic nucleotides (28).…”

mentioning

confidence: 99%

Proteolytic Inhibition ofSalmonella entericaSerovar Typhimurium-Induced Activation of the Mitogen-Activated Protein Kinases ERK and JNK in Cultured Human Intestinal Cells

2002

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Bromelain, a mixture of cysteine proteases from pineapple stems, blocks signaling by the mitogen-activated protein (MAP) kinases extracellular regulated kinase 1 (ERK-1) and ERK-2, inhibits inflammation, and protects against enterotoxigenic Escherichia coli infection. In this study, we examined the effect of bromelain on Salmonella enterica serovar Typhimurium infection, since an important feature of its pathogenesis is its ability to induce activation of ERK-1 and ERK-2, which leads to internalization of bacteria and induction of inflammatory responses. Our results show that bromelain dose dependently blocks serovar Typhimuriuminduced ERK-1, ERK-2, and c-Jun NH 2 -terminal kinase (JNK) activation in Caco-2 cells. Bromelain also blocked signaling induced by carbachol and anisomycin, pharmacological MAP kinase agonists. Despite bromelain inhibition of serovar Typhimurium-induced MAP kinase signaling, it did not prevent subsequent invasion of the Caco-2 cells by serovar Typhimurium or alter serovar Typhimurium -induced decreases in resistance across Caco-2 monolayers. Surprisingly, bromelain also did not block serovar Typhimuriuminduced interleukin-8 (IL-8) secretion but synergized with serovar Typhimurium to enhance IL-8 production. We also found that serovar Typhimurium does not induce ERK phosphorylation in Caco-2 cells in the absence of serum but that serovar Typhimurium-induced invasion and decreases in monolayer resistance are unaffected. Collectively, these data indicate that serovar Typhimurium-induced invasion of Caco-2 cells, changes in the resistance of epithelial cell monolayers, and IL-8 production can occur independently of the ERK and JNK signaling pathways. Data also confirm that bromelain is a novel inhibitor of MAP kinase signaling pathways and suggest a novel role for proteases as inhibitors of signal transduction pathways in intestinal epithelial cells.

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Bromelain protects piglets from diarrhoea caused by oral challenge with K88 positive enterotoxigenicEscherichia coli

Cited by 75 publications

References 14 publications

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Enzymological characterization of pineapple extract for potential application in oak tasar (Antheraea proylei J.) silk cocoon cooking and reeling

Proteolytic Inhibition ofSalmonella entericaSerovar Typhimurium-Induced Activation of the Mitogen-Activated Protein Kinases ERK and JNK in Cultured Human Intestinal Cells

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