Broadening of the 31P-NMR Signal of Vacuole-Associated Inorganic Phosphate by NaCl in Wild-Turf Roots

Takagishi, Hidejiro; Shirata, Kazuto; Ishida, Noriko; Kobayashi, Toshio; Koizumi, Masaru; Kano, Hiromi

doi:10.1016/s0176-1617(11)80232-4

Cited by 4 publications

(2 citation statements)

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“…Pitman et al (1981) found high concentrations of Na in the vacuole after salt stress and suggested that sequestration of Na into plant vacuoles prevents a toxic accumulation of this element in the cytosol. In salt tolerant wild-turf roots an increase in inorganic phosphate in vacuoles after salt stress has also been observed (Takagishi et al, 1991). Here it was assumed that inorganic phosphate functions as a counter-ion to Na in the ).…”

Section: mentioning

confidence: 94%

“…Knowledge of the compartmentation of K has been gained mainly from experiments with )'Rb as a tracer for K (Kasimir-Klemedtsson & Pettersson, 1991). The subcellular distribution of Na has been investigated by #$Na-NMR (Takagishi et al, 1991). However, information about the apoplastic contents or the intercellular distribution between plant and fungal tissues are not obtainable by these techniques.…”

Section: mentioning

confidence: 99%

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Subcellular compartmentation of elements in non‐mycorrhizal and mycorrhizal roots of Pinus sylvestris: an X‐ray microanalytical study. II. The distribution of calcium, potassium and sodium

Bücking

Heyser

2000

New Phytologist

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The inter‐ and intracellular distribution of the elements calcium, potassium and sodium in non‐mycorrhizal and mycorrhizal roots of Pinus sylvestris dependent on different external nutrient supply conditions was detected by energy‐dispersive X‐ray microanalysis after cryofixation, freeze‐drying and pressure infiltration of the material. In non‐mycorrhizal and mycorrhizal roots, calcium was mainly detectable in the apoplastic regions. The levels in vacuoles and cytoplasm were near the limits of detection by X‐ray microanalysis. Incubation with high concentrations of potassium and sodium, or mycorrhizal infection with Suillus bovinus and Pisolithus tinctorius reduced the amounts of calcium detectable in the roots, especially in the apoplast of cortical cells. The studies revealed that: potassium is mainly localized in cytoplasm and cell walls; the cytoplasmic content is regulated over a wide range of external potassium concentrations; potassium levels in the inner parts of roots are higher than in the outer parts. Mycorrhizal infection with Suillus bovinus had no effect on the inter‐ and intracellular distribution of potassium in roots but, if the external supply was low, the potassium content in shoots was reduced. In non‐mycorrhizal pine roots and those infected with Paxillus involutus an increase in the sodium content of all cell compartments was observed after treatment with high external concentrations of NaH2PO4. However, an increase in sodium content in mycorrhizas of S. bovinus was not detected. The X‐ray microanalytical results are discussed in relation to the apoplastic movement of nutrients in non‐mycorrhizal and mycorrhizal fine roots of pine and to the demand for these nutrients in different intracellular compartments.

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Section: mentioning

confidence: 94%

Section: mentioning

confidence: 99%