Effect of electric field on physical states of cell-associated water in germinating morning glory seeds observed by 1H-NMR

Effect of Electrical Current Applied in Soaking Conditions on Germination of Acacia and Maize Seeds

“…An study performed by Isobe et al (1999) showed that electric discharges applied to dry morning glory seeds can affect water absorption. They explained the effect in terms of changes in electrical polarization of cell membranes and the hydration of stored macromolecules.…”

“…Moreover, Shi et al (2014) indicated that high voltage electric field influence transmembrane potential affecting permeability of cell membrane. Isobe et al (1999) explained the negative effect of electrical current in terms of water distribution into the seeds. In their case, the treatment of morning glory seeds with electric field for 60 min alters water molecules in the cells, which were more restricted in motion when cells were treated with the discharges.…”

Cold- or Heat-Tolerance of Leaves and Roots in Perennial Ryegrass Determined by1H-NMR

“…In morning glory (Pharbitis nil Chois.) seeds, three components of water fractions characterized by different T 1 s and chemical shifts were observed (Isobe et al, 1999). T 1 of the two components in ryegrass leaves and roots linearly decreased as the temperature decreased from 20 to 0ºC (Fig.…”

“…Water in plant tissues is often characterized by different proton relaxation times (Ishida et al, 1987;Isobe et al, 1999; Iwaya-Inoue and Nonami, 2003). T 1 and T 2 have been used to detect the water components in parenchyma tissue of apple (Snaar and Van As, 1992).…”

Detection of Pre-Harvest Sprouting in Rice Seeds by Using 1H-NMR

“…The dynamic states of water in biological tissues were considered to reflect physiological changes. The components of T1 and T2 have been shown to arise from distinct populations of water in plant tissues (Gusta et al, 1979;Belton and Ratcliffe 1985;Isobe et al, 1999;Iwaya-Inoue and Nonami 2003). Thus, the NMR technique is expected to provide a novel, sensitive and direct method of characterizing water status in seeds.…”