Broad-specificity time-resolved fluorescent immunochromatographic strip for simultaneous detection of various organophosphorus pesticides based on indirect probe strategy

Dong, Haowei; Xu, Deyan; Wang, Guanjie; Meng, Xiaoya; Sun, Xia; Yang, Qingqing; Guo, Yemin; Zhu, Yelong

doi:10.1039/d2ay00067a

Cited by 8 publications

(2 citation statements)

References 28 publications

(36 reference statements)

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“…As shown in Table 5, the test strip detection method has been widely applied to the detection of pesticide residues. Now it can detect a variety of organophosphorus pesticides, and can perform the mixed rapid detection of various types [79][80][81]. Furthermore, these test results are consistent with the instrumental test results, although the stability is slightly lower.…”

Section: Pesticide Residuessupporting

confidence: 65%

The Developments on Lateral Flow Immunochromatographic Assay for Food Safety in Recent 10 Years: A Review

Wang,

Li,

Guo

et al. 2024

Chemosensors

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Food safety inspections are an essential aspect of food safety monitoring. Rapid, accurate, and low-cost food analysis can considerably increase the efficiency of food safety inspections. The lateral flow immunochromatographic assay (LFIA) technique has recently grown in popularity due to its ease of use and high efficiency. It is currently commonly utilized in food inspection. In this review, we briefly introduce the principle and classification of LFIA, critically discuss the recent application status of LFIA in food contaminantion detection, and finally propose that artificial intelligence and information technology will further advance the development of LFIA in the field of food safety monitoring.

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Section: Pesticide Residuessupporting

confidence: 65%

The Developments on Lateral Flow Immunochromatographic Assay for Food Safety in Recent 10 Years: A Review

Wang,

Li,

Guo

et al. 2024

Chemosensors

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“…The design of immunochromatographic test strip (ICTS) can be currently used for pathogens’ nucleic acid detection, and is called “nucleic acid lateral flow immunoassay” (NALFIA) ( Duong and Rhee, 2007 ; Chen et al, 2008 ; Chen et al, 2019 ; Zhuang et al, 2019 ; Chen et al, 2022 ). Immunochromatography test strip technology is currently widely used in fields requiring rapid antigen testing, especially in clinical medicine ( Lin et al, 2022 ), agriculture ( Dong et al, 2022 ; Lu et al, 2022 ), drug abuse ( Suryoprabowo et al, 2021 ), food ( Wang et al, 2022 ; Xing et al, 2022 ) and environmental semi-antigen pollutants ( Ren et al, 2021 ), and microbial pollutants ( Gao et al, 2021 ). In recent years, scientists have been working to develop ICTS methods for pathogen detection ( Li et al, 2020a ), but its low sensitivity has made it a bottleneck worldwide.…”

Section: Introductionmentioning

confidence: 99%

Rapid quantitative detection of Klebsiella pneumoniae in infants with severe infection disease by point-of-care immunochromatographic technique based on nanofluorescent microspheres

Chen

Sha²,

Li³

et al. 2023

Front. Bioeng. Biotechnol.

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Background:Klebsiella pneumoniae (KP, K. pneumoniae) is one of the most important nosocomial pathogens that cause severe respiratory infections. As evolutionary high-toxic strains with drug resistance genes increase year by year, the infections caused by it are often accompanied by high mortality, which may be fatal to infants and can cause invasive infections in healthy adults. At present, the traditional clinical methods for detecting K. pneumoniae are cumbersome and time-consuming, and the accuracy and sensitivity are not high. In this study, nanofluorescent microsphere (nFM)-based immunochromatographic test strip (ICTS) quantitative testing platform were developed for point-of-care testing (POCT) method of K. pneumoniae.Methods: 19 clinical samples of infants were collected, the genus-specific gene of mdh was screened from K. pneumoniae. Polymerase chain reaction (PCR) combined with nFM-ICTS based on magnetic purification assay (PCR-ICTS) and strand exchange amplification (SEA) combined with nFM-ICTS based on magnetic purification assay (SEA-ICTS) were developed for the quantitative detection of K. pneumoniae. The sensitivity and specificity of SEA-ICTS and PCR-ICTS were demonstrated by the existing used classical microbiological methods, the real-time fluorescent quantitative PCR (RTFQ-PCR) and PCR assay based on agarose gel electrophoresis (PCR-GE).Results: Under optimum working conditions, the detection limits of PCR-GE, RTFQ-PCR, PCR-ICTS and SEA-ICTS are 7.7 × 10−3, 2.5 × 10−6, 7.7 × 10−6, 2.82 × 10−7 ng/μL, respectively. The SEA-ICTS and PCR-ICTS assays can quickly identify K. pneumoniae, and could specifically distinguish K. pneumoniae samples from non-K. pneumoniae samples. Experiments have shown a diagnostic agreement of 100% between immunochromatographic test strip methods and the traditional clinical methods on the detection of clinical samples. During the purification process, the Silicon coated magnetic nanoparticles (Si-MNPs) were used to removed false positive results effectively from the products, which showed of great screening ability. The SEA-ICTS method was developed based on PCR-ICTS, which is a more rapid (20 min), low-costed method compared with PCR-ICTS assay for the detection of K. pneumoniae in infants. Only need a cheap thermostatic water bath and takes a short detection time, this new method can potentially serve as an efficient point-of-care testing method for on-site detection of pathogens and disease outbreaks without fluorescent polymerase chain reaction instruments and professional technicians operation.

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A novel strategy for identification of pesticides in different categories by concentration-independent model based on a nanozyme with multienzyme-like activities

Song

Lei

Tian

et al. 2023

Biosensors and Bioelectronics

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Broad-specificity time-resolved fluorescent immunochromatographic strip for simultaneous detection of various organophosphorus pesticides based on indirect probe strategy

Abstract: The massive use of organophosphorus pesticides (OPs) posed a great threat to food safety, human health and environmental protection. As there are many kinds of pesticides, the detection of them...

Cited by 8 publications

References 28 publications

The Developments on Lateral Flow Immunochromatographic Assay for Food Safety in Recent 10 Years: A Review

The Developments on Lateral Flow Immunochromatographic Assay for Food Safety in Recent 10 Years: A Review

Rapid quantitative detection of Klebsiella pneumoniae in infants with severe infection disease by point-of-care immunochromatographic technique based on nanofluorescent microspheres

A novel strategy for identification of pesticides in different categories by concentration-independent model based on a nanozyme with multienzyme-like activities

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