Broad frequency sensitivity and complex neural coding in the larval zebrafish auditory system

Poulsen, Rebecca; Scholz, Leandro Aluisio; Constantin, Lena; Favre‐Bulle, Itia A.; Vanwalleghem, Gilles; Scott, Ethan K.

doi:10.1016/j.cub.2021.01.103

Cited by 17 publications

(26 citation statements)

References 62 publications

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“…Images from the SPIM experiments were warped to a common template previously acquired and registered to the Zbrain atlas. The resulting warping parameters were applied to the xyz coordinates of the centroids of the ROIs to map them into the 294 brain regions defined in the Zbrain atlas ( Randlett et al, 2015 ; Poulsen et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…For visualizing the ROIs, we used Unity to represent each as a sphere and map it back to the brain. An isosurface mesh of the zebrafish brain was generated from the Zbrain masks for the diencephalon, mesencephalon, rhombencephalon, telencephalon and eyes using ImageVis3D71 ( Poulsen et al, 2021 ). The mesh was imported in Unity and overlaid to the ROIs.…”

Section: Methodsmentioning

confidence: 99%

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Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

Mancienne

Márquez-Legorreta

Wilde

et al. 2021

Front. Neural Circuits

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Animals from insects to humans perform visual escape behavior in response to looming stimuli, and these responses habituate if looms are presented repeatedly without consequence. While the basic visual processing and motor pathways involved in this behavior have been described, many of the nuances of predator perception and sensorimotor gating have not. Here, we have performed both behavioral analyses and brain-wide cellular-resolution calcium imaging in larval zebrafish while presenting them with visual loom stimuli or stimuli that selectively deliver either the movement or the dimming properties of full loom stimuli. Behaviorally, we find that, while responses to repeated loom stimuli habituate, no such habituation occurs when repeated movement stimuli (in the absence of luminance changes) are presented. Dim stimuli seldom elicit escape responses, and therefore cannot habituate. Neither repeated movement stimuli nor repeated dimming stimuli habituate the responses to subsequent full loom stimuli, suggesting that full looms are required for habituation. Our calcium imaging reveals that motion-sensitive neurons are abundant in the brain, that dim-sensitive neurons are present but more rare, and that neurons responsive to both stimuli (and to full loom stimuli) are concentrated in the tectum. Neurons selective to full loom stimuli (but not to movement or dimming) were not evident. Finally, we explored whether movement- or dim-sensitive neurons have characteristic response profiles during habituation to full looms. Such functional links between baseline responsiveness and habituation rate could suggest a specific role in the brain-wide habituation network, but no such relationships were found in our data. Overall, our results suggest that, while both movement- and dim-sensitive neurons contribute to predator escape behavior, neither plays a specific role in brain-wide visual habituation networks or in behavioral habituation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

Mancienne

Márquez-Legorreta

Wilde

et al. 2021

Front. Neural Circuits

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“…The hearing ability of fish, in general, is well established and considered to be important for their survival in the aquatic environment [36,[46][47][48]. However, their ability to discriminate between complex sounds has not been systematically tested [35,49]. NemoTrainer has the potential to be used to further examine this ability in almost any species.…”

Section: Audiovisual Learning In Zebrafishmentioning

confidence: 99%

NemoTrainer: Apparatus and Software for Automated Conditioning of Stimulus-directed Navigation and Decision Making in Freely Behaving Animals

Zu¹,

Summers²,

Asdjodi³

et al. 2022

Preprint

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Directed movement towards a target requires spatial working memory, including processing of sensory inputs and motivational drive. In a stimulus-driven, operant conditioning paradigm designed to train zebrafish, we present a pulse of light via LED’s and/or sounds via an underwater transducer. A webcam placed below a glass tank records fish swimming behavior. During operant conditioning, a fish must interrupt an infrared beam at one location to obtain a small food reward at the same or different location. A timing-gated interrupt activates robotic-arm and feeder stepper motors via custom software controlling a microprocessor (Arduino). “Ardulink”, a JAVA facility, implements Arduino-computer communication protocols. In this way, full automation of stimulus-conditioned directional swimming is achieved. Precise multiday scheduling of training, including timing, location and intensity of stimulus parameters, and feeder control is accomplished via a user-friendly interface. Our training paradigm permits tracking of learning by monitoring, turning, location, response times and directional swimming of individual fish. This facilitates comparison of performance within and across a cohort of animals. We demonstrate the ability to train and test zebrafish using visual and auditory stimuli. Current methods used for associative conditioning often involve human intervention, which is labor intensive, stressful to animals, and introduces noise in the data. Our relatively simple yet flexible paradigm requires a simple apparatus and minimal human intervention. Our scheduling and control software and apparatus (NemoTrainer) can be used to screen neurologic drugs and test the effects of CRISPR-based and optogenetic modification of neural circuits on sensation, locomotion, learning and memory.

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“…Particularly for spatial learning, the stimuli zones are located at different positions in the microfluidic devices to infuse the associated spatial information in the working organism. 3 Meanwhile, different types of positive 21,[33][34][35][36][37][38][39] and negative stimuli (motivators) [40][41][42][43][44][45] have been employed to investigate the associated behaviours of zebrafish larvae. A few positive stimuli, such as sight 33,34 and computer-based images, 35 have been employed to investigate the social behaviour of zebrafish larvae.…”

Section: Introductionmentioning

confidence: 99%

“…As one of the positive motivators, sound stimuli have been employed to train zebrafish larvae. 21,39 Under such stimuli, zebrafish has been observed to manifest a defence response called startle response. 46 Such response is arbitrated by Mauthner cells (M-cells), which are large reticulospinal neurons that acquire information from ipsilateral sensory afferents and synapses to contralateral spinal motor neurons.…”

Section: Introductionmentioning

confidence: 99%

Behavioural responses of zebrafish with sound stimuli in microfluidics

Loganathan

Chen

2023

Lab Chip

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Broad frequency sensitivity and complex neural coding in the larval zebrafish auditory system

Cited by 17 publications

References 62 publications

Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

NemoTrainer: Apparatus and Software for Automated Conditioning of Stimulus-directed Navigation and Decision Making in Freely Behaving Animals

Behavioural responses of zebrafish with sound stimuli in microfluidics

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