Brine Shrimp Teratogenic Screening Test on Some Selected Plants

Abstract

Objectives: The aims of this research were to analyze teratogenic properties on some selected plants which often used for woman’s health were pomegranate (Punica granatum L.) pericarp, coriander (Coriandrum sativum L.) seeds, liman (Elephantopus scaber L.) leaves, fennel (Foeniculum vulgare M.) seeds, and kaempferia (Kaempferia galanga L.) rhizomes using brine shrimp teratogenic screening test (BSTST) method.Methods: Artemia cysts were hatched into first stage nauplii then taken and put into seawater medium which contain test substance and kept alive until the second stage, third stage, and fourth stage and then observed number of deaths, morphological abnormalities, body length, and retarded of development for each stage.Results: Hatchability of cysts in fennel seeds compared extracts 1 and 0.5 mg/ml, coriander seeds extract 1 mg/ml, pomegranate rinds extract 6 mg/ml, and liman leaves extracts 6, 3, and 1.5 mg/ml were significantly different to control (p<0.05). Survival nauplii in fennel seeds extracts 1 and 0.5 mg/ml and liman leaves extracts 6 and 3 mg/ml were significantly different to control (p<0.05). The morphological abnormalities were exposed on coriander seeds extract 1 mg/ml, liman leaves extract 6 mg/ml, and pomegranate rinds extract 3 mg/ml. Nauplii with retarded development were presented on fennel seeds extracts 1 and 0.5 mg/ml, coriander seeds extracts 1 and 0.25 mg/ml, pomegranate rinds extracts 6, 3, and 1.5 mg/ml, and liman leaves extracts 3 and 1.5 mg/ml. Nauplii body length on plants extract showed no significantly difference compared to control.Conclusion: Based on BSTST, fennel seed and pomegranate rind extracts had no teratogenic effect, kaempferia rhizomes act as larvicide its analog in mamalia as embryo lethal, while coriander seeds and liman leaves extracts potentially had teratogenic properties.

Toxicological Evaluation of Pomegranate Fruit Extract in Male and Female Rats

“…Clinical observation, body weight, ocular examination, food consumption, and organ weight all had no statistically significant value. Differences from the control were seen in serum chemistry and hematology measures, but there was no evidence of a harmful effect on biological variation as presented by Lestari, Soemardji and Fidrianny [43], Patel, Dadhaniya, Hingorani and Soni [44].…”

The Effect of Liman Leaves Extract on Fetal Rat Development

“…The reproduction performance of rat's mother which was given by liman leaves extract at the 11 th day was similar to teratogenicity screening result using brine shrimp test (BST). The result of BST screening showed that high concentration of liman leaves extract (6 mg/ml) caused higher percentage of dead nauplii than the other concentrations, while developmental disorder was mostly found at low concentration (1.5 mg/ml) [18].…”

Novel Findings of Phoenix Dactylifera, Dates Fruit Early-Stage Hababuk for Phytochemical Screening, Antioxidant and Cytotoxic Potentials

“…Experiments were conducted along with control (vehicle-treated), different concentrations (100-1000 µg/ml) of the substances in a set of three tubes per dose. Based on the percent mortality, the LDR 50R of the test compound was determined using the probit scale [15].…”