Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants

Abstract

The present study was conducted to test for in vivo Brine Shrimp Lethality Assay (BSLA) of the Aqueous and ethanolic extracts of Annona reticulata with Allium fistolisum and Brassica oleraceaeand correlate cytotoxicity results with known pharmacological activities of the plants. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). Ten nauplii were added into three replicates of each concentration of the plant extract. After 24 h the surviving brine shrimp larvae were counted and LC50 was assessed. Results showed that the extracts of Annona reticulata with Allium fistolisumand Brassica oleraceaewere potent against the brine shrimp when compared alone with combined extracts. It indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine.

Green Synthesis of Nickel Oxide Nanoparticles from Berberis balochistanica Stem for Investigating Bioactivities

“…All statistical analyses were performed using Statistix version 10. The cytotoxic activity of BBS-NiONPs was estimated by calculating the lethality concentration (IC 50 ) values using the probit analysis (GraphPad software, San Diego, CA, USA) [ 46 , 47 ].…”

Euphorbia graminea Jacq. (Euphorbiaceae): In-Vitro Cytotoxic, Phytotoxic and Anti-Proliferative Assessment of the Extract and Fractions of the Leaves

“…The extract and fractions of E. graminea were screened for lethality using the Brine shrimp assay methods previously described. 11,12 Freshly hatched Nauplii were obtained from the Zoology Department of the University of Karachi, Pakistan and the experiment was carried out at the HEJ Research Laboratory, University of Karachi, Pakistan. The procedure involves the addition of 2 mL of distilled water to 20 mg of the extract.…”

<i>In-vitro</i> antioxidant, anti-inflammatory and cytotoxic activity of <i>Cucumis melo L. </i>of ethanolic extract

“…After 24 h of observation all the shrimp were survived in the control. Ogbole et al, (20) described that the ethanolic extract investigated displayed significant cytotoxic activity, with fruit extract of Cucumis melo L. showing cytotoxicity higher than the positive control (cyclophosphamide) (21).…”