Bright and photostable fluorescent probe with aggregation-induced emission characteristics for specific lysosome imaging and tracking

Ouyang, Jun; Zang, Qiguang; Chen, Wansong; Li, Shuo; Liu, Ren-Yu; Deng, Yuanyuan; Liu, Zhao‐Qian; Li, Juan; Liu, Deng

doi:10.1016/j.talanta.2016.06.031

Cited by 20 publications

(6 citation statements)

References 42 publications

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“…Therefore, tracers modified with a lysosomal targeting moiety are supposed to display enhanced uptake in legumain-overexpressed tumors, thereby giving high tumor/background contrast. The most used lysosome-targeting group is morpholine. − Thus, morpholine was introduced to develop a dual-targeting tracer [ 18 F] SF-AAN-M (Scheme ), which contains a morpholine and a legumain substrate sequence AAN. We anticipated that the dual-targeting effect might make more tracers selectively enter into and aggregate in the lysosome, resulting in more reliable PET imaging.…”

Section: Introductionmentioning

confidence: 99%

Dual-Targeting PET Tracers Enable Enzyme-Mediated Self-Assembly for the PET Imaging of Legumain Activity

Lu,

Li,

et al. 2023

ACS Appl. Mater. Interfaces

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Legumain, a lysosomal cysteine protease overexpressed in a variety of tumors, has been considered a promising biomarker for various cancers. Precise detection of legumain activity in the lysosome represents an important strategy for early diagnosis and prognosis of tumors. Small-molecule probes with the property of target-enabled self-assembly hold great potential for molecular imaging. In this study, we reported two dual-targeting radiotracers ([18F]SF-AAN-M and [18F]SF-AAN-HEM) with a property of legumain-mediated self-assembly for positron emission tomography (PET) imaging. Both the radiotracers were synthesized with high labeling yield (>50%) and the radiochemical purity was over 99% via one-step straightforward 18F-labeling. Both tracers were efficiently activated by the reducing agent and legumain to self-assemble into aggregates and showed enhanced retention in legumain-overexpressed MDA-MB-468 cells and tumors, indicating that the introduction of lysosome-targeting morpholine increased the tumor uptake and extended the retention of radiotracers in legumain-overexpressed tumors. In addition, [18F]SF-AAN-HEM with a hydrophilic (histidine-glutamate)3 tag displayed significantly reduced liver uptake with no conspicuous reduction in tumor uptake, affording high signal-to-noise ratios (tumor/liver and tumor/muscle). All of these results suggest that dual-targeting tracer [18F]SF-AAN-HEM could provide a promising tool for in vivo monitoring legumain activity in tumors.

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Section: Introductionmentioning

confidence: 99%

Dual-Targeting PET Tracers Enable Enzyme-Mediated Self-Assembly for the PET Imaging of Legumain Activity

Lu,

Li,

et al. 2023

ACS Appl. Mater. Interfaces

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“…[35] Besides, AIEgens with weak primary morpholine groups are more favored to enrich in the lysosome. [36] In recent decades, photoactivatable AIE materials with rational modified groups have been developed and widely used to image organelles precisely due to their unique advantages of photo-controlled emissive behaviors, including photostability, fast response, and high spatiotemporal resolution. [12][13][14]37] 3.…”

Section: Molecule-based Subcellular Imagingmentioning

confidence: 99%

Photoactivatable Biomedical Materials Based on Luminogens with Aggregation‐Induced Emission (AIE) Characteristics

Sun

et al. 2021

Adv Healthcare Materials

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Fluorescence probes with aggregation-induced emission (AIE) property are fascinating and vital in biological fields due to their bright fluorescence in the solid state. In contrast, traditional AIE materials are obscured by the off-target effects and lack of spatial and temporal control. Photoactivatable materials with AIE characteristics, whose physicochemical behaviors can be remotely activated by light, provide great potential in biochemical information acquisition with high spatial and temporal resolution. By using AIE-featured photoactivatable fluorescence probes, accurate analysis of the targets of interest is possible. For example, where, when, and to what extent a process is started or stopped by manipulating the non-invasive light accurately. Thus, many researchers are enthusiastic about developing AIE-featured photoactivatable materials and mainly focus on developing novel molecules by rational molecular structure design, and exploring advanced applications by appropriate molecular functionalization. In this review, the recent achievements of photoactivatable materials with AIE characteristics from the aspects involving inherent mechanism of photoactivity, molecular design strategy, and the corresponding applications in biological fields, are summarized. The biological applications are highlighted and discussed, including photoactivatable bioimaging, diagnosis, and photo-controlled therapy. Finally, the challenges and prospects of the AIE-featured photoactivatable materials are also outlined and discussed.

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“…20,21 In order to observe the dynamic changes of lysosomes, the probes must be continuously illuminated under a fluorescence microscope for a period of time and the relevant probe to lysosomes with better resistance to photobleaching is required. 22,23 Usually, researchers use higher concentrations of fluorophores to improve photostability. 24 Unfortunately, however, this approach doesn't work in most cases because of the accompanying concentration quenching effects.…”

Section: Introductionmentioning

confidence: 99%

Polymerization-induced emission (PIE) of multifunctional polyamides synthesized by Ugi polymerization and targeted imaging of lysosomes

et al. 2023

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Bright and photostable fluorescent probe with aggregation-induced emission characteristics for specific lysosome imaging and tracking

Cited by 20 publications

References 42 publications

Dual-Targeting PET Tracers Enable Enzyme-Mediated Self-Assembly for the PET Imaging of Legumain Activity

Dual-Targeting PET Tracers Enable Enzyme-Mediated Self-Assembly for the PET Imaging of Legumain Activity

Photoactivatable Biomedical Materials Based on Luminogens with Aggregation‐Induced Emission (AIE) Characteristics

Polymerization-induced emission (PIE) of multifunctional polyamides synthesized by Ugi polymerization and targeted imaging of lysosomes

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