Bridelia ferruginea Benth leaves attenuates diabetes nephropathy in STZ-induced rats via targeting NGAL/KIM-1/cystatin c gene

Abstract

Background: Renal failure is among the main complications of diabetes disorders and free radical from hyperglycemia is the major cause of this nephrotic complication. This study investigated the therapeutic effects of Bridelia ferruginea Benth on nephrotic damage in streptozotocin (STZ) induced diabetic rats. Diabetes mellitus was induced by injection of 50 mg/kg b. w. of STZ (intraperitoneal) in rats fasted overnight. Effect on nephropathy was assessed using biochemical, histological indices and gene expression of kidney tissue. Results: The total flavonoids and phenolic content of petroleum ether fraction of B. ferruginea (PEFBF) was observed to be higher compared to diethyl ether fraction of B. ferruginea (DEFBF). The 1,1-diphenyl-2-picryl-hydrazyl scavenging activity of the fractions were significantly (P < 0.05) reduced across the concentrations compared to the standard (gallic acid). The STZinduced diabetic animals treated with 100 and 200 mg/kg b.w. of PEFBF and DEFBF showed significant (P < 0.05) reduction in blood glucose level on days 7, 11 and 14 compared to untreated diabetic rats. STZ-induced diabetic rats significantly (P < 0.05) exhibited increase plasma urea, creatinine, protein and albumin level while treatment with both fractions of B. ferruginea reduced the level of these parameters in treated diabetic rats. B. ferruginea also caused down-regulation of neutrophil gelatinase-associated lipocalin, kidney injury molecule-1 and cystatin c genes. Conclusion: These results revealed that, the plant has hypoglycemic activity therefore provides a pharmacological basis for its folkloric use in the management of hyperglycemia and its associated renal dysfunction.

Potent pigmentation inhibitory activity of incensole‐enriched frankincense volatile oil‐identification, efficacy and mechanism

“…DPPH scavenging activity of FVO was evaluated according to the method reported by Omoboyowa et al 12 with slight modification. Briefly, 4 mL of 500 μg/mL‐5000 μg/mL of the ethanol solution of FVO was added to 4 mL of DPPH solution (50 mg of DPPH in 100 mL of Ethanol).…”

“…DPPH scavenging activity of FVO was evaluated according to the method reported by Omoboyowa et al 12 with slight modification.…”

Antidiabetes study of Spondias mombin (Linn) stem bark fractions in high-sucrose diet-induced diabetes in Drosophila melanogaster

“…The scavenging capacity of 1,1-diphenyl-2-picrylhydrazyl ( DPPH) of the fractions was evaluated according to the procedure described by Omoboyowa et al. 15 with slight modification. Briefly, 1 mL of 250–1000 μg/mL of the tested compounds was added to 1 mL DPPH reagent (2.4 mg DPPH prepared with 200 mL methanol).…”