Brevibacillus as a biological tool: a short review

Panda, Amrita Kumari; Bisht, Satpal Singh; DeMondal, Surajit; Kumar, Nachimuthu Senthil; Gurusubramanian, Guruswami; Panigrahi, Ashok Kumar

doi:10.1007/s10482-013-0099-7

Cited by 65 publications

(48 citation statements)

References 111 publications

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“…with promising antimicrobial activity. The genus Brevibacillus was established in 1996 on the basis of a genetic reclassification of strains previously recognized to be Bacillus brevis (8). Many bioactive compounds have been isolated from Brevibacillus spp.…”

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Isolation and Structural Elucidation of Brevibacillin, an Antimicrobial Lipopeptide from Brevibacillus laterosporus That Combats Drug-Resistant Gram-Positive Bacteria

Huang

Yuan

et al. 2016

Appl Environ Microbiol

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A new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant Staphylococcus aureus, vancomycin-resistant strains of Enterococcus faecalis and Lactobacillus plantarum, and other Gram-positive bacteria. The producer strain, designated OSY-I 1 , was determined to be Brevibacillus laterosporus via morphological, biochemical, and genetic analyses. The antimicrobial agent was extracted from cells of OSY-I 1 with isopropanol, purified by high-performance liquid chromatography, and structurally analyzed using mass spectrometry (MS) and nuclear magnetic resonance (NMR). The MS and NMR results, taken together, uncovered a linear lipopeptide consisting of 13 amino acids and an N-terminal C 6 fatty acid (FA) chain, 2-hydroxy-3-methylpentanoic acid. The lipopeptide (FA-Dhb-Leu-Orn-Ile-Ile-Val-Lys-Val-Val-Lys-Tyr-Leu-valinol, where Dhb is ␣,␤-didehydrobutyric acid and valinol is 2-amino-3-methyl-1-butanol) has a molecular mass of 1,583.0794 Da and contains three modified amino acid residues: ␣,␤-didehydrobutyric acid, ornithine, and valinol. The compound, designated brevibacillin, was determined to be a member of a cationic lipopeptide antibiotic family. In addition to its potency against drugresistant bacteria, brevibacillin also exhibited low MICs (1 to 8 g/ml) against selected foodborne pathogenic and spoilage bacteria, such as Listeria monocytogenes, Bacillus cereus, and Alicyclobacillus acidoterrestris. Purified brevibacillin showed no sign of degradation when it was held at 80°C for 60 min, and it retained at least 50% of its antimicrobial activity when it was held for 22 h under acidic or alkaline conditions. On the basis of these findings, brevibacillin is a potent antimicrobial lipopeptide which is potentially useful to combat drug-resistant bacterial pathogens and foodborne pathogenic and spoilage bacteria. U nregulated access to antibiotics is one of the main reasons for the spread of antibiotic-resistant pathogens and their resistance genes through migration, travel, and trade (1). It was reported that in Europe alone, 25,000 patients die annually because of bacterial infections which cannot be treated with common antibiotics (2). Examples of antibiotic-resistant bacteria are methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus spp., carbapenem-resistant Mycobacterium tuberculosis, and highly virulent multidrug-resistant Clostridium difficile strains (2-5). Therefore, the discovery and development of new antimicrobial agents are of paramount importance. Despite their natural scarcity, new antimicrobial agents can be discovered by subjecting microorganisms that potentially produce such agents to screening and isolation processes (6, 7).The current study led to the discovery of a new strain of Brevibacillus sp. with promising antimicrobial activity. The genus Brevibacillus was established in 1996 on the basis of a genetic reclassification of strains previously recognized to be Bacillus brevis (8). Many bioactive compounds have been...

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“…Many bioactive compounds have been isolated from Brevibacillus spp. since then, including new antibacterial, antifungal, and anti-invertebrate agents (8,9). For instance, extracellular neutral protease that controls nematode growth was obtained from a Brevibacillus sp.…”

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confidence: 99%

Isolation and Structural Elucidation of Brevibacillin, an Antimicrobial Lipopeptide from Brevibacillus laterosporus That Combats Drug-Resistant Gram-Positive Bacteria

Huang

Yuan

et al. 2016

Appl Environ Microbiol

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“…The genus Bacillus and related genera are reported to be widely distributed in nature. It includes thermophilic, psychrophilic, acidophilic, alkaliphilic and halophilic bacteria that utilize a wide range of carbon sources for heterotrophic growth or are the autotrophs (Panda et al, 2014). In the present study, the isolates were recovered from five hot springs in Eritrea with different temperature regimes (49 -100°C), as well as different sodium concentration levels ranging from 0.06 to 3640 mg/lt at near neutral pH.…”

Section: Discussionmentioning

confidence: 99%

Thermotolerant bacteria of biotechnological potential from hot springs in Eritrea

Ghilamicael¹,

Budambula²,

Anami³

et al. 2018

Afr. J. Microbiol. Res.

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Thermophiles are excellent sources of enzymes that can withstand and carry out reactions efficiently under high temperatures. This study isolated and characterised thermotolerant bacteria that produce enzymes of potential industrial value from five hot springs in Eritrea. A total of 65 bacterial isolates were obtained from the five hot springs. Out of the 65 isolates; 19 isolates produced a positive reaction for amylases, 36 for carboxymethyl cellulases, eight for proteases, 10 for xylanases and 11 for pectinases. More than half (36 out of 65) were able to produce carboxymethyl cellulases. Six isolates which showed carboxymethyl cellulase activity were from the genus Bacillus, while those belonging to Brevibacillus were seven. BLAST analysis of the partial sequences showed that 19 out of the 24 isolates sequenced showed high similarity (> 99%) to those of reference strains of the genera Bacillus and Brevibacillus available in the Genebank and EZ-taxon databases. The five isolates (E5, G2, G8, M1 and M13) that showed moderate similarities (97.2-99%) to strains from the Genebank and EZ-taxon databases were further characterized. Physiological characterization of the five selected isolates based on tolerance to NaCl, temperature and production of hydrolytic enzymes indicated that these isolates are potentially novel. Isolates G8 and M13 showed significantly higher amylase activity (p < 0.05) than the other three isolates. Caseinase activity recorded by the five isolates was the highest (p < 0.05) compared to other enzyme activities. The enzymes produced by thermotolerant bacteria from the five hot springs may be potentially useful for catalysis under harsh operational conditions encountered in industrial processes.

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“…G ram-positive bacteria of the genus Brevibacillus are widespread in various habitats [1]. They are characterized by a high growth rate, better transformation efficiency using electroporation, the presence of transport vectors, the production of a small amount of extracellular protease and the constitutive expression of heterologous proteins [1]. Representatives of this genus are of great biotechnological interest -they are a source of various enzymes, antitumor peptides, and are capable of biodegrading polyethylene [1,2].…”

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Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain

Гудзенко¹,

Волювач²,

Горшкова³

et al. 2020

Ukr.Biochem.J

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Biotechnological treatment of enterprises wastewater, which contains toxic phenolic compounds, is the most acceptable method today. therefore, the search for new non-pathogenic biochemically active microorganisms -destructors of phenol and their identification is relevant. the aim of this study was to determine phenol-oxidizing activity and the fatty acid profile of Brevibacillus centrosporus F14 -destructor of phenol, isolated from pharmaceutical plant wastewater. Fatty acids analysis of the microorganism culture was carried out using automatic system of microorganisms identification Midi sherlock based on gas chromatograph agilent 7890. the phenol-oxidizing activity of bacteria was determined by photometric method according to the degree of phenol extraction from the water. it was experimentally confirmed that Brevibacillus sp. F14 strain had a phenol-destructive activity. When phenol-containing water was treated with Brevibacillus sp. F14 cells, phenol concentration in the water decreased from 200.0 ± 12.0 mg/l to 6.8 ± 0.8 mg/l during 15 days of exposure. chromatographic analysis showed that saturated fatty acids i-14:0 (14.9%), i-15:0 (14.8%), a-15:0 (34.9%), i-16:0 (11.10%) dominated in the strain's fatty acids profile. the total content of the branched saturated fatty acids i-14:0, i-15:0, a-15: 0, i-16: 0, i-17: 0, a-17:0, ∑(a-17:1/i-17:1) was 85.0% and that of saturated and unsaturated fatty acids with the normal structure -7% of the total fatty acids pool. the distinction of Brevibacillus centrosporus F14 strain's fatty acid profile from other bacteria of Brevibacillus genus was the presence of more i-14:0 (14.85%), i-16:0 (11.10%) fatty acids and 16: 1 w7c alcohol (7.71%), as well as the minor content of fatty acids markers 16:0 n alcohol (0.60%), 18:3 w6c (0.4%) and ∑(a-17:1/i-17:1) (1.94%). according to the phenotypic characteristics and fatty acid composition, the tested phenol-oxidizing strain belongs to Brevibacillus centrosporus species. the index of Brevibacillus centrosporus F14 strain's fatty acid profile similarity with the library was 0.645. k e y w o r d s: Brevibacillus centrosporus, destructor of phenol, identification, composition of fatty acids. position of a wide range of substrates, including polyethy lene (plastic) and long-chain hydrocarbons. These genomic features may be useful for future environmental/biotechnological applications. Brevibacillus borstelensis has great potential to destroy carbendazim fungicide in crops and crops at a high rate, especially in combination with Streptomyces albogriseolus. The lipolytic strain of Brevibacillus borstelensis SH168 may enhance the conversion of food waste into bio-fertilizer.It is known that the bacterial strains, Brevibacillus centrosporus and paenibacillus azoreducens (have been isolated from the sludge samples col-doi: https://doi.

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Brevibacillus as a biological tool: a short review

Cited by 65 publications

References 111 publications

Isolation and Structural Elucidation of Brevibacillin, an Antimicrobial Lipopeptide from Brevibacillus laterosporus That Combats Drug-Resistant Gram-Positive Bacteria

Isolation and Structural Elucidation of Brevibacillin, an Antimicrobial Lipopeptide from Brevibacillus laterosporus That Combats Drug-Resistant Gram-Positive Bacteria

Thermotolerant bacteria of biotechnological potential from hot springs in Eritrea

Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain

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