Breeding Strategies to Increase Genetic Variability Within Hibiscus Syriacus

Laere, Katrijn Van; Huylenbroeck, Johan Van; Bockstaele, Erik Van

doi:10.17660/actahortic.2006.714.9

Cited by 12 publications

(16 citation statements)

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“…The results suggest that artificial genome doubling of diploid apple genotypes decreased plant vigour. Although polyploidization in plants is often associated with increased vigour, e.g., triploid apples (Sedysheva, Gorbacheva 2013) and hexaploid Hibiscus (Van Laere et al 2006, artificial genome doubling is very frequently associated with a decreased growth rate, e.g., in Citrus (Allario et al 2011;Guerra et al 2014), Spathiphyllum (Van Laere et al 2011, Platanus (Liu et al (Stupar et al 2007), and tetraploid Zea mays (Riddle et al 2006). Several observations show the increased vigour of triploid apple cultivars (Sedysheva, Gorbacheva 2013) and triploid Zea mays (Riddle et al 2006) over diploids but decreased vigour of higher ploidy levels and own observations of an increased vigour in tetraploid azalea; however, decreased vigour of hexaploids compared to diploids (Eeckhaut et al 2006) suggests that plant species potentially possess an optimal ploidy degree, below or above which its growth will be reduced.…”

Section: Discussionmentioning

confidence: 99%

Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

Hias¹,

Leus²,

Davey³

et al. 2017

Hortic. Sci.

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Hias N., Leus L., Davey M.W., Vanderzande S., Van Huylenbroeck J., Keulemans J. (2017): Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes. Hort. Sci. (Prague), 44: 55-63.Because polyploidy often results in enhancement of desirable properties, artificial genome doubling is commonly used in agri-and horticultural crop breeding programs. In this study genome doubling was induced in two apple genotypes. The effect on vegetative morphological and physiological traits of the plants was then comprehensively determined by comparing the obtained tetraploid apple plants with their diploid counterparts. Out of 17 different physio-and morphological characteristics, 15 were significantly affected in one or both genotypes. The response of these 15 characteristics also appeared to have been caused by two effects; 10 of the 15 characteristics exhibited a common response to ploidy change over both genotypes while five traits showed a genotype-specific response to polyploidization. Tetraploid leaves also exhibited a darker leaf colour, which could be correlated to a higher pigment concentration. Furthermore, the results also show a decreased elongation rate and leaf size in tetraploids, which is suggested to be due to the observed lower cell density in the polyploid apple plants.

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Section: Discussionmentioning

confidence: 99%

Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

Hias¹,

Leus²,

Davey³

et al. 2017

Hortic. Sci.

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“…Application of oryzalin on 3 d (consecutive) yielded one octoploid and one cytochimera from the 100 and 150 mM treatments, respectively. Van Laere et al (2006) treated seedlings of Hibiscus syriacus L. 'Oiseau Blue' and 'Woodbride' for 10 d (consecutive) with 0.2% colchicine + 2% DMSO to obtain 24.5% and 41.8% doubling in the two cultivars, respectively. The greater success obtained in the study by Van Laere et al (2006) may be attributed to the longer treatment duration.…”

Section: Discussionmentioning

confidence: 99%

“…Van Laere et al (2006) treated seedlings of Hibiscus syriacus L. 'Oiseau Blue' and 'Woodbride' for 10 d (consecutive) with 0.2% colchicine + 2% DMSO to obtain 24.5% and 41.8% doubling in the two cultivars, respectively. The greater success obtained in the study by Van Laere et al (2006) may be attributed to the longer treatment duration. Furthermore, a lower concentration of agar and preventing the agar droplet from desiccating may have allowed greater uptake of oryzalin.…”

Section: Discussionmentioning

confidence: 99%

An Oryzalin-induced Autoallooctoploid of Hibiscus acetosella ‘Panama Red’

Contreras¹,

Ruter²,

Hanna³

2009

J. Amer. Soc. Hort. Sci.

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Hibiscus acetosella Welw. ex Hiern. ‘Panama Red’ PP20,121 (Malvaceae) has generated public and grower interest due to its attractive red foliage and vigorous growth, however, a horticultural goal is to develop more compact forms. Even though organs of induced polyploids are often larger than the wild type, whole plants are often shorter in stature. Three studies were conducted to induce polyploidy and to evaluate the growth and reproductive potential of the resulting polyploids. In study 1, seeds were soaked for 24 hours in aqueous solutions of 0%, 0.2%, 0.4%, or 0.5% colchicine (w/v) plus 0.5% dimethyl sulfoxide. In studies 2 and 3, apical meristems of seedlings at the cotyledon stage were treated for 1 or 3 days with 0, 50, 100, or 150 μm oryzalin solidified with 0.8% agar. Visual observations and measurement of guard cells were used to identify plants that potentially had their chromosome number doubled. Flow cytometry of nuclei stained with DAPI was used for confirmation of polyploidy. No induced polyploidy was observed following seed treatment with colchicine at the rates and duration used in this study. One-time application of 50 μm oryzalin resulted in a single mixoploid (4x + 8x) in which the ploidy of the L-I, L-II, and L-III histogenic layers were identified as a 4–4-4 + 8, respectively. Three-day applications with 100 and 150 μm oryzalin resulted in an octoploid (8x) and a mixoploid (4x + 8x), respectively. The mixoploid from the 3-day treatment stabilized at the 8x level before flowering, but was identified as a 4 + 8-x-4 cytochimera. Plant height was reduced, leaves were smaller, internodes were shorter, and canopy volume was reduced in the octoploid (8x) form compared with the tetraploid (4x) form. Furthermore, in contrast to the tetraploid, the octoploid produced no self-pollinated seed and performed poorly as a staminate and pistillate parent in controlled crosses. This represents the first time oryzalin has been reported to induce polyploidy in Hibiscus L. section Furcaria DC. H. acetosella is an allotetraploid species with the genome composition AABB. The resulting autoallooctoploid (AAAABBBB) form of ‘Panama Red’ exhibits a more compact habit and reduced production of seed.

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“…For example, Buddleja globosa (2n = 2x = 38) was chromosome doubled in order to make interspecific hybridization with Buddleja davidii (2n = 4x = 76) (Rose et al 2000a;Van Laere 2008).…”

Section: Applications Of In Vitro Chromosome Doubling In Plant Breedingmentioning

confidence: 99%

“…Induction of tetraploid plants might be a step towards obtaining sterile triploid cultivars, resulting from a cross between a diploid and a chromosome doubled plant, as was the case in Musa (Bakry et al 2007), Weigela (Arène et al 2007) and Hibiscus (Van Laere et al 2006).…”

Section: Applications Of In Vitro Chromosome Doubling In Plant Breedingmentioning

confidence: 99%

Mitotic chromosome doubling of plant tissues in vitro

Dhooghe¹,

Laere²,

Eeckhaut³

et al. 2010

Plant Cell Tiss Organ Cult

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258

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In vitro chromosome doubling can be induced by several antimitotic agents. The most commonly used are colchicine, oryzalin and trifluralin. The process of induced chromosome doubling in vitro consists of a typical succession of sub-processes, including an induction phase and a confirmation protocol to measure the rate of success. The induction step depends on a large number of variables: media, antimitotic agents, explant types, exposure times and concentrations. Flow cytometry is the pre-eminent method for evaluation of the induced polyploidization. However, alternative confirmation methods, such as chromosome counts and morphological observations, are also used. Since polyploidization has many consequences for plant growth and development, chromosome doubling has been intensively studied over the years and has found its way to several applications in plant breeding. This review gives an overview of the common methods of chromosome doubling in vitro, the history of the technique, and progress made over the years. The applications of chromosome doubling in a broader context are also discussed.

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Breeding Strategies to Increase Genetic Variability Within Hibiscus Syriacus

Cited by 12 publications

References 0 publications

Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

An Oryzalin-induced Autoallooctoploid of Hibiscus acetosella ‘Panama Red’

Mitotic chromosome doubling of plant tissues in vitro

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