Breeding of SooOkJam an Artificial Diet Adaptable Silkworm Variety, for Spring Rearing Season

Abstract

A new silkworm variety SooOkJamfor spring rearing season is F 1 hybrid between Jam157, a japanese race bred from introduction breeding and Jam158, a chinese race from introduction breeding. In the local adaptability test performed at 8 local areas in spring of 2011, the hatchability rate of SooOkJam was recorded 2% higher than the authorized silkworm variety, DaePoongJam. The larval period was recorded 12 hour shorter than DaePoongJam. The pupation percentage was recorded 0.8% higher than DaePoongJam. Single cocoon weight (2.40 g), Cocoon yield (22.6 kg) and best cocoon rate (95.5%) was similar to DaePoongJam. The concentration of DNJ was measured 4.12 mg in SooOkJam, lower than DaePoongJam (4.45 mg). Test of the artificial diet of SooOkJam showed excellent adaptability of the artificial diet for larval period from 1st to 3rd, similar to KumOkJam. In the Paecilomyces tenuipes production ability test, the pupal weight of SooOkJam was 1.61 g/individual, similar to DaePoongJam (1.65 g/individual).

Molecular diagnosis of 5 silkworm strains endemic to South Korea using single-nucleotide polymorphisms selected from whole-genome sequences

“…A collection of 39 premium silkworm strains, including 5 endemic strains (~15 individuals per strain), was procured from the National Academy of Agricultural Science, Rural Development Administration, South Korea ( Table 1 ). The specifics of the culture conditions are comprehensively outlined in previous work by Kang et al (2012) . For the extraction of genomic DNA for amplicon sequencing, thoracic tissue samples were individually homogenized from 8 to 10 specimens of third or fourth-instar larvae per strain using micro pestle.…”

“…Historically, the strategy of intra-strain breeding within a single species, Bombyx mori , has inevitably constrained the development of strain-specific diagnostic markers ( Kim et al 2008 , 2010 , 2014 ). This constraint is more pronounced given that the evolution of various silkworm strains has predominantly focused on quantitative traits, including larval period, body color, cocoon attributes, and resistance to environmental variables ( Kang et al 2003 , 2012 ). However, these traits are primarily useful for classifying large quantities of resources into overarching categories, rather than facilitating strain identification.…”