Breeding of Rosa Rugosa Thunb. Using Different Tissue Culture Methods

Jakobsone, Gunta; Rieksta, D.; Rihtere, A.

doi:10.17660/actahortic.2006.725.25

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2009

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“…At 36.16 lM 2,4-D, all explants formed calluses with no organized structures. The frequency of embryogenic callus formation on mature zygotic embryos (zygotic embryo explants) in this study was approximately six-fold higher than that on immature zygotic embryos reported by Kunitake et al (1993) and Jakobsone et al (2006). In preliminary studies, we also cultured immature zygotic embryos in the same manner as we cultured mature zygotic embryo explants described above, which then gave rise to no somatic embryos.…”

Section: Resultsmentioning

confidence: 68%

Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose

Kim

Liu

2009

Plant Biotechnol Rep

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Rugosa rose (Rosa rugosa) is cultivated as a garden flower and an important genetic resource for the breeding of roses (R. hybrida). This study describes culture conditions for high frequency plant regeneration from zygotic embryo explants via somatic embryogenesis in rugosa rose. Mature zygotic embryo, cotyledon, and radicle explants formed embryogenic calluses at frequencies of 38, 6.7, and 8.8% when cultured on half-strength Murashige and Skoog medium (MS) supplemented with 2.26, 9.05, and 9.05 lM 2,4-dichlorophenoxyacetic acid, respectively. Embryogenic calluses produced numerous somatic embryos, which then developed into plantlets on MS without growth regulators. Regenerated plantlets were grown to whole plants in a growth chamber.

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Section: Resultsmentioning

confidence: 68%