Breeding of Resistant Strawberry Cultivars for Organic Fruit Production - Preliminary Results With Botrytis Cinerea

Bestfleisch, Markus; Höfer, Monika; Hanke, Magda‐Viola; Flachowsky, Henryk; Richter, Klaus; Schulte, E.

doi:10.17660/actahortic.2013.976.8

Cited by 5 publications

(7 citation statements)

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“…The study by Klatt et al . () showed that concentrations of the stimulant compound of B. cinerea conidial germination cis ‐3‐hexenyl acetate emitted by ‘Sonata’ and ‘Darselect’ strawberry flowers were 6·2 and 0·6 ng g −1 , respectively, and ‘Sonata’ strawberry was found to be more susceptible to B. cinerea than ‘Darselect’ strawberry (Bestfleisch et al ., ). The results in the present investigation, of fungal stimulation by low concentrations of 6‐methyl‐5‐hepten‐2‐one, β‐ionone, nonanal, hexanal, trans ‐2‐hexanal, hexyl acetate, butyl acetate and 2‐methylbutyl acetate are in agreement with previous reports in the literature for several pathogens (French, ; Hamilton‐Kemp et al ., ; Fallik et al ., ; Filonow, ).…”

Section: Discussionmentioning

confidence: 97%

Role of strawberry volatile organic compounds in the development of Botrytis cinerea infection

et al. 2014

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Botrytis cinerea, the main pathogen of strawberry, has the ability to remain quiescent in unripe tissue and develop disease symptoms in ripe fruit. As strawberry ripening is characterized by an increase of aroma compounds, the role of volatile emission in the development of infection was investigated. Thirty-five strawberry volatile organic compounds (VOCs) were tested on B. cinerea in vitro and volatile emission was analysed in strawberry harvested at four ripening stages by headspace solid-phase microextraction/gas chromatography-mass spectrometry and proton transfer reactiontime of flight-mass spectrometry. The coupling of such data sets made it possible to conclude that key strawberry aroma compounds stimulate B. cinerea conidial germination and some typical wound-volatiles stimulate pathogen conidial germination or mycelial growth. This study is the first report of fungal stimulation by some VOCs naturally occurring in strawberry: the esters ethyl butanoate, cis-3-hexenyl acetate, trans-2-hexenyl acetate, methyl butanoate and hexyl butanoate, the furanones furaneol and mesifurane, and the alcohol trans-2-hexenol. The results of this work provide advances in understanding the functional role of fruit VOCs and suggest, for the first time, that fruit VOCs may influence the development of B. cinerea from the latent phase and that they could favour the invasive growth of B. cinerea after wounding. In particular, ethyl butanoate and furaneol could signal strawberry ripening, and the green leaf volatiles trans-2-hexenol, trans-2-hexenyl acetate and cis-3-hexenyl acetate could signal the presence of damaged tissues that are easier sites for penetration by B. cinerea.

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Section: Discussionmentioning

confidence: 97%

Role of strawberry volatile organic compounds in the development of Botrytis cinerea infection

et al. 2014

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“…A standardized test under laboratory conditions can lead to more reproducible data and can therefore be used effectively for evaluation of strawberry germplasm and cultivar descriptions. Different inoculation methods and incubation conditions tested revealed that the best reproducible results could be obtained by artificial inoculation of fruits under laboratory conditions (Bestfleisch et al, 2013). The resistance to B. cinerea is quantitative and influenced by environmental factors as well as by general plant and fruit robustness.…”

Section: Discussionmentioning

confidence: 99%

“…As a standardized method for the determination of resistance of strawberries towards B. cinerea , the method previously described by Bestfleisch et al . () was modified for high throughput and exact daily measurement of fruits and fungal infestation. The fruits that had fully ripened, according to Commission Regulations (EU) No.…”

Section: Methodsmentioning

confidence: 99%

“…Numerous studies have been published which focus on characterization of strawberry genetic resources and breeding material for their resistances towards Botrytis fruit rot (Irvine & Fulton, 1959;Jarvis & Borecka, 1968;Barritt, 1980;Bristow et al, 1986;Simpson, 1991;Chandler et al, 2006;Seijo et al, 2008;Bestfleisch et al, 2013). Most of these investigations have used natural infections in the field; meaning their comparability is very limited.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of strawberry (Fragaria L.) genetic resources for resistance to Botrytis cinerea

et al. 2014

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The grey mould disease caused by Botrytis cinerea leads to substantial economic losses in strawberry production all over the world. Control of the disease requires an extensive amount of fungicide that is applied in varying complexes because the pathogen easily develops resistance against the active compounds. Planting of resistant cultivars seems to be a promising alternative for fruit growers, but there are currently no cultivars available combining resistance to B. cinerea with attractive horticultural traits. Breeding of new cultivars requires the effective identification of resistant strawberry genotypes; therefore the current study was aimed at the evaluation of strawberry genetic resources under controlled conditions by establishing an artificial inoculation assay. The method presented in this study is an artificial inoculation of ripe fruits with a defined spore suspension under laboratory conditions. The results show that this assay is fast and simple and leads to reproducible results that correlate with field observations. Over 3 years a total of 107 strawberry genotypes of the German National Fruit Genebank at the JKI in Dresden‐Pillnitz were evaluated. Five partly resistant genotypes, cultivars Diana, Joerica and Kimberly, and Fragaria virginiana ‘Wildmare Creek’ and F. vesca subsp. bracteata, were identified with mean disease levels of <20% at 6 days post‐inoculation. The obtained results are discussed with regard to future breeding activities.

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“…Understanding the basis of non-additive inheritance has prospects to further improve hybrid breeding, and the effects of non-additive inheritance patterns have been Andrés Eduardo Rodriguez Cubillos and Hao Tong have equally contributed to this work and are first authors Zoran Nikoloski and Roosa A. E. Laitinen have equally contributed to this work and are corresponding authors traditionally studied in several crop species (Alseekh et al 2015;Bestfleisch et al 2013;Boer et al 2007;Riedelsheimer et al 2012a;Schauer et al 2008). So far, studies on natural variation among global Arabidopsis thaliana accessions have helped us understand genetic mechanisms underlying genes that cause differential fitness in hybrids in comparison to the parents (Weigel 2012) together with the genetic basis of non-additive inheritance patterns (Seymour et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Inheritance patterns in metabolism and growth in diallel crosses of Arabidopsis thaliana from a single growth habitat

et al. 2017

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Metabolism is a key determinant of plant growth and modulates plant adaptive responses. Increased metabolic variation due to heterozygosity may be beneficial for highly homozygous plants if their progeny is to respond to sudden changes in the habitat. Here, we investigate the extent to which heterozygosity contributes to the variation in metabolism and size of hybrids of Arabidopsis thaliana whose parents are from a single growth habitat. We created full diallel crosses among seven parents, originating from Southern Germany, and analysed the inheritance patterns in primary and secondary metabolism as well as in rosette size in situ. In comparison to primary metabolites, compounds from secondary metabolism were more variable and showed more pronounced non-additive inheritance patterns which could be attributed to epistasis. In addition, we showed that glucosinolates, among other secondary metabolites, were positively correlated with a proxy for plant size. Therefore, our study demonstrates that heterozygosity in local A. thaliana population generates metabolic variation and may impact several tasks directly linked to metabolism.

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Breeding of Resistant Strawberry Cultivars for Organic Fruit Production - Preliminary Results With Botrytis Cinerea

Cited by 5 publications

References 0 publications

Role of strawberry volatile organic compounds in the development of Botrytis cinerea infection

Role of strawberry volatile organic compounds in the development of Botrytis cinerea infection

Evaluation of strawberry (Fragaria L.) genetic resources for resistance to Botrytis cinerea

Inheritance patterns in metabolism and growth in diallel crosses of Arabidopsis thaliana from a single growth habitat

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