Breeding of high protein Chlorella sorokiniana using protoplast fusion

Xie, Fucun; Feng-feng, Zhang; Zhou, Ke; Zhao, Qiong; Sun, Haixuan; Wang, Shu; Zhao, Yujie; Fu, Jinran

doi:10.1016/j.biortech.2020.123624

Cited by 18 publications

(6 citation statements)

References 43 publications

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“…The HPLC conditions were as follows: separation column: Eclipse Plus C18 (250 mm × 4.6 mm, 5 μm); mobile phase: tetrahydrofuran/methanol/acetonitrile (5:45:50, V / V / V ); detector: UV detector; detection wavelength: 446 nm; column temperature: 30 °C; flow rate: 1.0 mL/min. The protein was determined using an automatic Kjeldahl analyzer (K9860, Manon, Dezhou, China) according to the method of Xie et al [ 30 ]. Firstly, 10 mL of nitric acid was added to 0.1 g of algal flour and 0.5 g of Kjeldahl catalyst sheet (potassium sulfate/copper sulfate = 15:1).…”

Section: Methodsmentioning

confidence: 99%

Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Fang

Hong³

et al. 2022

Foods

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High-cost carbon sources are not economical or sustainable for the heterotrophic culture of Chlorella vulgaris. In order to reduce the cost, this study used sweet sorghum extract (SE) and its enzymatic hydrolysate (HSE) as alternative carbon sources for the heterotrophic culture of Chlorella vulgaris. Under the premise of the same total carbon concentration, the value-added product production performance of Chlorella vulgaris cultured in HSE (supplemented with nitrogen sources and minerals) was much better than that in the glucose medium. The conversion rate of the total organic carbon and the utilization rate of the total nitrogen were both improved in the HSE system. The biomass production and productivity using HSE reached 2.51 g/L and 0.42 g/L/d, respectively. The production of proteins and lipids using HSE reached 1.17 and 0.35 g/L, respectively, and the production of chlorophyll-a, carotenoid, and lutein using HSE reached 30.42, 10.99, and 0.88 mg/L, respectively. The medium cost using HSE decreased by 69.61% compared to glucose. This study proves the feasibility and practicability of using HSE as a carbon source for the low-cost heterotrophic culture of Chlorella vulgaris.

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Section: Methodsmentioning

confidence: 99%

Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Fang

Hong³

et al. 2022

Foods

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“…For example, the PEG fusion method was used to fuse a high-protein mutant (H10) with a fast-growing mutant (Z13) to produce a mutant that displayed increased protein and amino acid content, as well as rapid growth. The total protein content of one of the fusions R7 (67.16%) was 8.89 and 10.25% higher than that of the original strains H10 and Z13, respectively (133). Although the fibrous cell wall of C. sorokiniana (a microalgae) poses a challenge for the preparation of protoplast fusion, different enzyme combinations and concentration treatments can be implemented for effective cell disruption (133,134).…”

Section: Protoplast Fusionmentioning

confidence: 99%

“…The total protein content of one of the fusions R7 (67.16%) was 8.89 and 10.25% higher than that of the original strains H10 and Z13, respectively (133). Although the fibrous cell wall of C. sorokiniana (a microalgae) poses a challenge for the preparation of protoplast fusion, different enzyme combinations and concentration treatments can be implemented for effective cell disruption (133,134).…”

Section: Protoplast Fusionmentioning

confidence: 99%

Developing algae as a sustainable food source

et al. 2023

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Current agricultural and food production practices are facing extreme stress, posed by climate change and an ever-increasing human population. The pressure to feed nearly 8 billion people while maintaining a minimal impact on the environment has prompted a movement toward new, more sustainable food sources. For thousands of years, both the macro (seaweed and kelp) and micro (unicellular) forms of algae have been cultivated as a food source. Algae have evolved to be highly efficient at resource utilization and have proven to be a viable source of nutritious biomass that could address many of the current food production issues. Particularly for microalgae, studies of their large-scale growth and cultivation come from the biofuel industry; however, this knowledge can be reasonably translated into the production of algae-based food products. The ability of algae to sequester CO2 lends to its sustainability by helping to reduce the carbon footprint of its production. Additionally, algae can be produced on non-arable land using non-potable water (including brackish or seawater), which allows them to complement rather than compete with traditional agriculture. Algae inherently have the desired qualities of a sustainable food source because they produce highly digestible proteins, lipids, and carbohydrates, and are rich in essential fatty acids, vitamins, and minerals. Although algae have yet to be fully domesticated as food sources, a variety of cultivation and breeding tools exist that can be built upon to allow for the increased productivity and enhanced nutritional and organoleptic qualities that will be required to bring algae to mainstream utilization. Here we will focus on microalgae and cyanobacteria to highlight the current advancements that will expand the variety of algae-based nutritional sources, as well as outline various challenges between current biomass production and large-scale economic algae production for the food market.

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“…Protoplast fusion is an effective way to construct genetically engineered strains [37]. It is also frequently used in microorganism breeding for the large-scale enhancement of metabolite yields.…”

Section: Introductionmentioning

confidence: 99%

High-Value Utilization of Tea Forest Resources: Breeding Eurotium cristatum Strains to Enhance Lovastatin Yields in Anhua Dark Tea

Li,

Liu,

Liu

et al. 2023

Forests

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The tea plant is a vital strategic forest resource in China. Dark tea produced from its leaves is an indispensable health-promoting product in western China due to its unique lipid-lowering function. Eurotium cristatum is the dominant strain in Fuzhuan brick tea (a variety of Anhua dark tea) and could produce many functional components, including lovastatin, a lipid-lowering compound. In this study, the lovastatin yield of dark tea was improved by breeding Eurotium cristatum using the protoplast fusion method. The experiments were carried out by inducing a fusion between inactivated Eurotium cristatum JH1205 and Monascus CICC5031. Among the 92 fusants screened the HPLC method, four strains (A4, A36, A54, and A76) with higher lovastatin production (more than three times as high) were obtained. The A76 strain had the highest lovastatin yield, which was 23.93 μg/mL. The location of the tea forest strongly influenced the lovastatin yield of loose dark tea. The strain bred in this study improved the lovastatin yield of loose dark tea by more than three times when compared to wild Eurotium cristatum. These results are promising for the development of tea forest resources.

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Breeding of high protein Chlorella sorokiniana using protoplast fusion

Cited by 18 publications

References 43 publications

Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract

Developing algae as a sustainable food source

High-Value Utilization of Tea Forest Resources: Breeding Eurotium cristatum Strains to Enhance Lovastatin Yields in Anhua Dark Tea

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