Cocoa (Theobroma cacao L.) is the third important beverage from Amazon forest basin. The processed seeds are used for the production of chocolate, cocoa powder and cocoa butter. The cross pollinating nature coupled with existence of self/cross incompatibility poses much difficulty to the cocoa breeders. Variation within the population is the basis for the selection. The cocoa inbreds of various genotypes belonging to different generations, maintained at Cocoa Research Centre (CRC) were evaluated from 2016-2019. In the S1, S2, generation of M 18.7 genotype positive inbreeding depression was observed for 17 and 16 characters respectively, whereas, maximum inbreeding depression (10%) was observed for wet bean weight per pod. In the S1, S2 and S3 generation of G II 7.4 genotype, positive inbreeding depression was observed for 10,15 and 7 characters respectively, whereas, highest negative inbreeding depression (-92.45 per cent) for ridge thickness i.e., high ridge thickness was observed in S4 generation. In the S1 and S2 generation of G IV 35.7, positive inbreeding was observed in 16 and 15 characters respectively. The similar trend of inbreeding depression in various genotypes has shown both negative and positive inbreeding depression. The inbreeding depression in cocoa inbreds over generation was not stable. ridge thickness and bean thickness, yield per pod per tree, pod index and phenol content have shown negative inbreeding depression indicating that these characters are controlled by additive genes.