Breeding Behavior and Histological Structure of a Nondehiscent Anther Character in Sorghum vulgare Pers.1

Webster, O. J.; Singh, S. P.

doi:10.2135/cropsci1964.0011183x000400060032x

Cited by 30 publications

(13 citation statements)

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“…It therefore, appears that the persistence of tapetal cells is not necessarily associated with male sterility and, therefore, some abnormality other than the tapetal degenera tion also exists in some A-lines leading to the male sterility. However, these investi gations and similar studies undertaken by previous researchers (Fillion andChristie 1966, Webster andSingh 1964) suggest that endothecium also play a major role in as much as all the pollen fertile lines possessed very thin endothecium compared to very thick endothecium in A-lines (Figs. 1 and 2).…”

Section: Methodssupporting

confidence: 86%

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Cytohistological Studies on Certain Male Sterile Lines of Pearl Millet (Pennisetum typhoides S. and H.)

Reddy¹,

Reddi²

1974

CYTOLOGIA

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Though cytoplasmic male sterility in pearl millet is of recent origin, several male sterile lines have been developed in the U. S. A. and also in India (Athwal 1966). Of late there have been few instances of breakdown of male sterility resulting in pollen shedding in Tift-23A, a male-sterile line (Reddy and Reddi 1970). Cyto histological studies on anthers of two male-sterile, and their corresponding maintainer lines and one pollen shedding line were made to assess the role of anther wall com ponents on the expression of male sterility. The effect of nitrogen on the develop ment of anther wall components was also studied. Materials and methodsAnthers of male-sterile lines ms Tift-23A and ms-628A (possessing different cytoplasms)3 and their maintainer lines Tift 23B and 628B were used in these studies. The crop was raised during December, 1970-February, 1971. Spikelets for meiosis were fixed at boot leaf stage in Farmer's fluid (Johansen 1940) and after 24 hours, they were stored in 70% ethanol until use. Acetocarmine smears were prepared to study the stages of meiosis.Spikelets for histological studies were collected at the following three stages of development:

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Section: Methodssupporting

confidence: 86%

“…In A-lines the situation was reverse in that it was thinner at stage-I but rapidly increased in thickness towards maturation (Table 1). The increase in thickness of endothecium has contributed to the non-dehiscence of anthers in A-lines as was observed in orchard grass (Fillion and Christie 1966) and sorhgum (Webster and Singh 1964).…”

Section: Methodsmentioning

confidence: 75%

Cytohistological Studies on Certain Male Sterile Lines of Pearl Millet (Pennisetum typhoides S. and H.)

Reddy¹,

Reddi²

1974

CYTOLOGIA

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“…A second type of cytoplasmic male sterility is caused by non dehiscence of anthers and has been developed from progenies of crosses of the Martin line with an African Sorghum variety 9E [23]. In this case fertility can be restored by nuclear genes carried in the Feterita varieties but not by Milo [23] indicating that the cytoplasm of 9E is probably different from that of Milo.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial gene expression and cytoplasmic male sterility in sorghum

Dixon

Leaver

1982

Plant Mol Biol

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Variation in sorghum mitochondrial translation products has enabled fertile (Kafir) cytoplasm to be distinguished from Milo cytoplasmic male sterile cytoplasm and from three alternative sources of cytoplasmic male sterile cytoplasm. Mitochondria from Milo cytoplasm synthesised a 65 000 mol. wt. polypeptide which was not synthesised by those from Kafir cytoplasm. In the cytoplasmic male sterile combination of Kafir nucleus in Milo cytoplasm synthesis of this polypeptide was dramatically increased. Mitochondria from two cytoplasmic male sterile lines (Kafir nucleus in IS1112 cytoplasm and Yellow Feterita nucleus in M35-1 cytoplasm) did not synthesise the 65 000 mol. wt. polypeptide but synthesised additional high molecular weight polypeptides (from 54 000 to 82 000 mol. wt.), the major one being 82 000. Mitochondria from cytoplasm IS1112 were also distinguished by synthesis of an additional 12 000 mol. wt. polypeptide. Mitochondria from the cytoplasmic male sterile line Martin nucleus in 9E cytoplasm synthesised an additional 42 000 mol. wt. polypeptide but did not synthesise a 38 000 mol. wt. polypeptide detected in all other cytoplasms. Immunoprecipitation of mitochondrial translation products with antiserum raised against subunit I of yeast cytochrome oxidase tentatively identified the 38 000 mol. wt. polypeptide as subunit I of sorghum cytochrome oxidase. The 42 000 mol. wt. polypeptide was also immuno-precipitated by this antiserum and thus is probably an altered form of cytochrome oxidase subunit I.Analysis of native mitochondrial DNA by agarose gel electrophoresis revealed the presence of two 'plasmid-like' DNA species of molecular weight 5.3 and 5.7 kb in the cytoplasmic male sterile lines Kafir nucleus in cytoplasm IS1112 and Yellow Feterita nucleus in M35-1 cytoplasm. Thus there is a positive correlation between the synthesis of the 82 000 mol. wt. polypeptide and the presence of the additional DNA species.

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“…Differences have also been reported in endothecial development in male fertile and male sterile strains (Pritchard and Hutton, 1972) and it was shown that thick endothecium gradually becomes attenuated to facilitate dehiscence in male fertile sorghum (Webster and Singh 1964), orchard grass (Fillion and Christie 1966) and pearl millet (Reddy andReddi 1970, 1974). In sterile material no such phenomenon was obterved.…”

Section: Discussionmentioning

confidence: 94%

Microsporogenesis in Male Sterile and Male Fertile Crotalaria pallida Ait

Arora

Gupta

1984

CYTOLOGIA

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Breeding Behavior and Histological Structure of a Nondehiscent Anther Character in Sorghum vulgare Pers.¹

Abstract: T HIS paper reports the breeding behavior of a nondehiscent anther character which first appeared in a cross involving an African sorghum variety. Anatomical and histological differences between normal and nondehiscent anthers are also presented. MATERIALS AND METHODS

Cited by 30 publications

References 0 publications

Cytohistological Studies on Certain Male Sterile Lines of Pearl Millet (<i>Pennisetum typhoides</i> S. and H.)

Cytohistological Studies on Certain Male Sterile Lines of Pearl Millet (<i>Pennisetum typhoides</i> S. and H.)

Mitochondrial gene expression and cytoplasmic male sterility in sorghum

Microsporogenesis in Male Sterile and Male Fertile <i>Crotalaria pallida</i> Ait

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