Breed-Specific Detection of Mangalica Meat in Food Products

Szántó-Egész, R.; Jánosi, A.; Mohr, Alfaro; Szalai, Gábor; Szabó, Erzsébet; Micsinai, Adrienn; Sipos, Rita; Rátky, J.; Anton, István; Zsolnai, Attila

doi:10.1007/s12161-015-0261-0

Cited by 19 publications

(16 citation statements)

References 13 publications

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“…Altogether 711 samples have been tested by RPA previously (SZÁNTÓ-EGÉSZ et al, 2016). A smaller set, 70 samples has been selected to test LAMP reaction in triplicates (Table 1.).…”

Section: Resultsmentioning

confidence: 99%

“…Each technique has its pros and cons. As for sample preparation, RPA is extremely insensitive to the quality of the DNA (KERSTING et al, 2014), even homogenised, unprocessed sausage sample can serve as a template for RPA reaction (SZÁNTÓ-EGÉSZ et al, 2016). Although LAMP produced successful amplifi cation from raw Mangalitza liver paté (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…DNA sources like liver paté or sausage and muscle samples were processed as described by SZÁNTÓ-EGÉSZ and co-workers (2016) using Wizard ® procedure (Promega, USA). Simple grinding and homogenisation were also applied parallel on liver paté and sausage (SZÁNTÓ-EGÉSZ et al, 2016) to test LAMP sensitivity to sample impurity.…”

Section: Samplesmentioning

confidence: 99%

“…For Mangalitza detection, portable Recombinase Polymerase Amplifi cation (RPA) approach has been developed (SZÁNTÓ-EGÉSZ et al, 2016), which requires a device capable of maintaining 39 °C and a lateral fl ow strip with easy yes/no indication of the successful amplifi cation.…”

mentioning

confidence: 99%

“…To test LAMP capability to identify Mangalitza specifi c DNA, we designed primers and tested against our previously described RPA method (SZÁNTÓ-EGÉSZ et al, 2016).…”

mentioning

confidence: 99%

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Loop-mediated isothermal amplification based approach as an alternative to recombinase polymerase amplification based detection of Mangalitza component in food products

Zsolnai

Szántó-Egész²,

Ferencz-Elblinger

et al. 2017

Acta Alimentaria

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We used an alternative approach, loop-mediated isothermal amplifi cation, to detect Mangalitza component in food products, and it has been compared to an established Recombinase Polymerase Amplifi cation test. The correlation between the assays was signifi cant (P<0.01). Linear determination coeffi cient between the assays was 0.993 and level of diagnostic agreement was high (Kappa=0.971).Previously, a real-time PCR method based on TaqMan probe was developed for detection of Mangalitza meat in food products, using a Mangalitza specifi c sequence. Other Mangalitza specifi c sequences suitable for the same purpose are also in use (V. STÉGER, personal communication).Approaches like real-time monitoring of accumulation of the specifi c DNA product usually require specialised laboratory equipment. For Mangalitza detection, portable Recombinase Polymerase Amplifi cation (RPA) approach has been developed (SZÁNTÓ-EGÉSZ et al., 2016), which requires a device capable of maintaining 39 °C and a lateral fl ow strip with easy yes/no indication of the successful amplifi cation.We wanted to develop another fast, non-PCR based test with minimal laboratory requirement to provide a third possibility to detect Mangalitza component in food.Keywords: food, LAMP, RPA, MangalitzaNowadays, a wide range of non-PCR amplifi cations are available, such as Helicase Dependent Amplifi cation (VINCENT et al., 2004), Transcription Mediated Amplifi cation (GUATELLI et al., 1990), Self-Sustained Sequence Replication (3SR) (GUATELLI et al., 1990), Rolling Circle Amplifi cation (FIRE and XU, 1995), Standard Displacement Amplifi cation (SDA) (WALKER et al., 1992), and loop-mediated isothermal amplifi cation (LAMP) (NOTOMI et al., 2000). They usually do not require expensive instrumentation and have comparable diagnostic power to * To whom correspondence should be addressed. E-mail: attila.zsolnai@gmail.com This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium for non-commercial purposes, provided the original author and source are credited, a link to the CC License is provided, and changes -if any -are indicated.

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“…Altogether 711 samples have been tested by RPA previously (SZÁNTÓ-EGÉSZ et al, 2016). A smaller set, 70 samples has been selected to test LAMP reaction in triplicates (Table 1.).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Samplesmentioning

confidence: 99%

mentioning

confidence: 99%

“…To test LAMP capability to identify Mangalitza specifi c DNA, we designed primers and tested against our previously described RPA method (SZÁNTÓ-EGÉSZ et al, 2016).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Loop-mediated isothermal amplification based approach as an alternative to recombinase polymerase amplification based detection of Mangalitza component in food products

Zsolnai

Szántó-Egész²,

Ferencz-Elblinger

et al. 2017

Acta Alimentaria

Self Cite

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Probabilistic genetic identification of wild boar hybridization to support control of invasive wild pigs (Sus scrofa)

Smyser,

Pfaffelhuber,

Giglio

et al. 2024

Ecosphere

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The rapid expansion of wild pigs (Sus scrofa) throughout the United States has been fueled by unlawful introductions, with invasive populations causing extensive crop losses, damaging native ecosystems, and serving as a reservoir for disease. Multiple states have passed laws prohibiting the possession or transport of wild pigs. However, genetic and phenotypic similarities between domestic pigs and invasive wild pigs—which overwhelmingly represent domestic pig and wild boar hybrids—pose a challenge for the enforcement of such regulations. We sought to exploit wild boar ancestry as a common attribute among the vast majority of invasive wild pigs as a means of genetically differentiating wild pigs from breeds of domestic pig found within the United States. We organized reference high‐density single nucleotide polymorphism genotypes (1039 samples from 33 domestic breeds and 382 samples from 16 wild boar populations) into five genetically cohesive reference groups: mixed‐commercial breeds, Durocs, heritage breeds, primitive breeds, and wild boar. Building upon well‐established genetic clustering approaches, we structured the test statistic to describe the difference in the likelihood of a given genotype's ancestry vectors (sensu genetic clustering analysis) if derived strictly from the four described domestic pig reference groups versus allowing for admixture from the wild boar group. By fitting statistical distributions to test statistics of reference domestic pigs, we characterized the distribution of the null hypothesis that a given genotype descends strictly from domestic pig reference groups. We tested the approach with simulated genotypes and empirical data from an additional 29 breeds of domestic pig represented by 435 unique genotypes; all associated test statistics for simulated and empirical domestic pig challenge sets fell within the distribution of reference domestic pigs. We then evaluated 6566 invasive wild pigs sampled across the contiguous United States, of which 63% exceeded the maximum threshold for domestic pigs and could be statistically classified as possessing wild boar ancestry. This approach provides a scientific foundation to enforce regulations prohibiting the possession of this destructive invasive species. Further, this computationally efficient and generalizable approach could be readily adapted to quantify gene flow among ecological systems of conservation or management concern.

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Real-Time Recombinase Polymerase Amplification Assay for the Detection of Vibrio cholerae in Seafood

Tang

Cao

et al. 2017

Food Anal. Methods

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Breed-Specific Detection of Mangalica Meat in Food Products

Cited by 19 publications

References 13 publications

Loop-mediated isothermal amplification based approach as an alternative to recombinase polymerase amplification based detection of Mangalitza component in food products

Loop-mediated isothermal amplification based approach as an alternative to recombinase polymerase amplification based detection of Mangalitza component in food products

Probabilistic genetic identification of wild boar hybridization to support control of invasive wild pigs (Sus scrofa)

Real-Time Recombinase Polymerase Amplification Assay for the Detection of Vibrio cholerae in Seafood

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