Breed characteristics associated with LEP gene polymorphisms in Holstein cattle

Machulskaya, E. V.; Kovalyuk, N. V.; Gorkovenko, L. G.; Shakhnazarova, Yu. Yu.; Morkovkina, N. A.

doi:10.3103/s1068367417040097

Cited by 3 publications

(2 citation statements)

References 2 publications

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“…According to Cattle QTL Database, we found some QTL associated with production, meat and carcass traits were located in chromosome regions intersected with these three genes (Table S6). Previous studies also showed that polymorphisms within LEP were associated with growth and meat quality traits in bovine (Carvalho et al, 2012;Machulskaya, Kovalyuk, Gorkovenko, Shakhnazarova, & Morkovkina, 2017;Negahdary et al, 2014). For TNF, only sporadic reports have shown that polymorphisms of TNF could be strongly related to reproductive traits (Shirasuna et al, 2011).…”

Section: Discussionmentioning

confidence: 95%

Tissue expression and variation analysis of three bovine adipokine genes revealed their effect on growth traits in native Chinese cattle

Chen

Han

et al. 2018

Reprod Domestic Animals

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Adipokines play a crucial role in the regulation of energy homeostasis; however, little is known about genetic alterations in this family that may contribute to economic traits in cattle. Therefore, this study conducts transcript profiles, variations and association studies of three major adipokines, leptin (LEP), tumour necrosis factor (TNF) and angiopoietin-like protein 8 (ANGPTL8), to evaluate their effects on native Chinese cattle. Using quantitative real-time PCR, the study revealed that the bovine LEP was expressed primarily in the back and visceral fat, while TNF was predominantly expressed in spleen and ANGPTL8 was mainly expressed in back fat and liver. Five single nucleotide polymorphisms (SNPs) including two missense SNPs (SNP1: g.12254T>C and SNP2: g.14177C>T) in LEP, a synonymous SNP (SNP3: g.2130A>G) in TNF and two SNPs (SNP4: g.629G>A and SNP5: g.884T>C) in the 5'UTR of ANGPTL8 were identified and genotyped in 537 individuals from six Chinese cattle breeds. Bioinformatics analysis revealed that SNP1 might disrupt the efficient binding of LEP to its receptor, SNP3 might affect translation efficiency of TNF, and SNP4 and SNP5 were likely to affect stability, splicing and nuclear export of ANGPTL8 mRNA. Consistently, association studies indicated that three SNPs (SNP1, SNP3 and SNP5) were significantly associated with body weight, heart girth, average daily gain, hip width and body length in 100 Nanyang cattle (p < 0.05). Overall, our results support the view that LEP, TNF and ANGPTL8 could be used as biomarkers to improve the growth performance in Chinese cattle selection programmes.

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Section: Discussionmentioning

confidence: 95%

Tissue expression and variation analysis of three bovine adipokine genes revealed their effect on growth traits in native Chinese cattle

Chen

Han

et al. 2018

Reprod Domestic Animals

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“…Эти результаты показывают, что генотип лептина TT связан с увеличением окружности грудной клетки. Эти сведения могут представлять экономический интерес [ 17].…”

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Polymorphism of Lipid Metabolism Genes in Tatarstan-Type Cows

Lamara,

Ahmetov,

Shaydullin

et al. 2024

Agrobiotechnologies and Digital Farming

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The purpose of the research was to study the allelic polymorphism of lipid metabolism genes (OLR1, DGAT1 and LEP) in Tatarstan type cows. The object of the study was 79 first-calf heifers of the Kholmogory breed of the Tatarstan type, kept in the agricultural production complex “Agrofirm Rassvet” in the Kukmorsky district of the Republic of Tatarstan. As a result of molecular genetic studies (PCR-RFLP and AS-PCR), the animals were divided into groups taking into account their genotype at the loci of the low-density lipoprotein receptor (OLR1), diacylglycerol-O-acyltransferase (DGAT1) and leptin (LEP) genes. Animals with different genotypes of the studied genes were further divided into groups taking into account their linear affiliation. The studied sample of first-calf heifers consisted of individuals belonging to two leading genealogical lines of the Holstein breed, namely: Wis Ideal 933122 and Reflection Sovering 198998. In general, the studies showed that in the herd of first-calf heifers of the Tatarstan type, predominated allele C (0.73) and genotype CC 49.4% for OLR1 gene; allele A (0.77) and genotype AA 57.0% for DGAT1 gene; allele C (0.59) and genotype CT 57.0% for LEP gene. In the sample under study, the genetic balance is not shifted for any of the studied genes. In cows, the frequency of occurrence of alleles of the OLR1, DGAT1 and LEP genes, depending on linear affiliation (W. Ideal and R. Sovering), the trend remains. Similar data were obtained that, taking into account belonging to the Holstein breed, the highest occurrence of alleles in first-calf heifers: C (0.68-0.81) for OLR1 gene, A (0.74-0.83) for DGAT1 gene and C (0.57 -0.60) for LEP gene, respectively.

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Milk Productivity and Milk Quality of Cows With Different Lep Genotypes and Linear Affiliation

Lamara,

Zarubezhnova,

Ahmetov

et al. 2023

Agrobiotechnologies and Digital Farming

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A review of the scientific literature has shown that the genotype by loci of the Leptin (LEP) gene and linear affiliation to the leading Holstein breed of cows have an impact on milk productivity and the quality of their milk. The purpose of our research was to study the milk productivity and qualitative composition of the milk of Tatarstan-type cows with different genotypes according to the loci of the Leptin gene and linear belonging to the Holstein breed. The research was carried out in the conditions of the agricultural company "Agrofirma Rassvet" of the Kukmorsky district of the Republic of Tatarstan on the livestock of 79 cows of the Tatarstan type. The Tatarstan type is one of the leading dairy cattle breeds bred in the Republic of Tatarstan, and created by crossing the Kholmogorsky, Black and White, and Holstein breeds, while the bloodline was 1/8 Kholmogorsky + 1/16 Black and White + 13/16 Holstein. As a result of a molecular genetic study (PCR-RFLP), the animal population was sorted into groups, taking into account the genotype by the loci of the Leptin gene. The conducted studies have shown that in Tatarstan-type cows after the first lactation, the highest milk yield indicators, the amount of fat and protein in milk were noted in animals with the leptin gene genotypes LEP/CC and LEP/CT in comparison with peers of the LEP/TT genotype. However, in terms of the mass fraction of fat and protein in milk, the trend was reversed. Also, among the cows of the Tatarstan type, according to the first lactation, the highest values of milk productivity indicators (milk yield, amount of milk fat and protein) are in cows with the LEP/CC genotype of linear belonging to the Holstein breed, namely W. Ideal 933122 and R. Sovering 198998.

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Breed characteristics associated with LEP gene polymorphisms in Holstein cattle

Cited by 3 publications

References 2 publications

Tissue expression and variation analysis of three bovine adipokine genes revealed their effect on growth traits in native Chinese cattle

Tissue expression and variation analysis of three bovine adipokine genes revealed their effect on growth traits in native Chinese cattle

Polymorphism of Lipid Metabolism Genes in Tatarstan-Type Cows

Milk Productivity and Milk Quality of Cows With Different Lep Genotypes and Linear Affiliation

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