Breast Cancer

Ferrero, Jean Marc; Namer, M.

doi:10.1007/978-3-642-78866-6_6

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…1 It is worth mentioning that breast cancer incidence is increasing at 1.5% per annum and represents almost 20% of all cancer types in woman and is the commonest cause of cancer death for women. 2 Colorectal cancer (CRC) presents a serious public health problem: it is the second leading cause of cancer mortality in the modern world, next to a lung cancer. Each year there are nearly one million new cases of CRC diagnosed worldwide and half a million deaths.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of circulating tumor cells of breast cancer and colorectal cancer from normal human mononuclear cells using Raman spectroscopy

Ranc

Srovnal

Kvı́tek

et al. 2013

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Circulating tumor cells present an important marker of the progress of several cancer diseases including breast and colorectal cancer, and enables an interesting prognosis and diagnostic options that can complement convenient diagnostic techniques based on several imaging methods. Based on its relevance, the analysis of such kinds of cells is within the scope of many research and clinical institutes; however, it still presents a difficult task. Here we used a state-of-the-art micro-Raman microscopic technique to enhance possibilities in the study of circulating tumor cells and their further differentiation. As cytospins present a convenient form of sample collection and preparation, we used this form of sampling as the initial point. Raman presents a non-destructive form of sample analysis and thus the samples can be further used for a method validation. We have considered the influence of fixation methods of the cells, where we found out that the ability of Raman spectroscopy to differentiate between three cell lines strictly depends upon the sample preparation method used. Namely breast (BT 549) and colorectal (HCT 116) circulating tumor cell lines and human mononuclear cells were compared. Methanol and paraformaldehyde methods of fixation were compared to simple drying out of the sample. It has been shown that drying out of the cells enables the best performance to be obtained in cell differentiation and this is demonstrated by the use of principal component analysis, where all three given cell lines were differentiated with a high level of confidence. Next, the cells were also scanned using 1 μm spatial resolution. The acquired data were visualized using both chemigrams and hierarchical clustering analysis.

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Section: Introductionmentioning

confidence: 99%

Discrimination of circulating tumor cells of breast cancer and colorectal cancer from normal human mononuclear cells using Raman spectroscopy

Ranc

Srovnal

Kvı́tek

et al. 2013

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A Clinical Trial to Selectively Change Dietary Fat and/or Energy Intake in Women: The Women's Diet Study

Djurić¹,

Uhley²,

Depper³

et al. 1999

Nutrition and Cancer

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Dietary fat and energy intake have been implicated in breast cancer etiology. To examine the relative importance of these dietary factors on markers of cancer risk in women, we designed an intervention trial to selectively decrease fat and/or energy intake in free-living, premenopausal women who were somewhat overweight. The study used a 2 x 2 factorial design to evaluate the independent and interactive effects of dietary fat and energy. The diets were nonintervention, low fat (15% of energy from fat, maintenance of energy intake), low energy (25% energy reduction), and combination low fat and low energy. We utilized an individualized counseling approach with self-selection of foods. Women on the low-fat and combination diets were asked to meet given daily goals for fat grams and food group exchanges, while women on the low-energy diet used only food group exchanges. Of the 113 premenopausal women randomized who were eligible for analysis, 43% were African-American. A total of 88 women completed the 12-week program, and adherence to the dietary goals was similar in both racial groups. Women on the low-fat diet were able to reduce dietary fat intake to 19% of energy by 4 weeks and to 17% by 12 weeks with a slight decrease in energy intake. Women on the low-energy diet met their energy reduction goals by four weeks while maintaining percentage of energy from fat. Women on the combination diet largely met their goals by four weeks as well. These data indicate that it is possible to selectively manipulate dietary fat and energy intake in women over a short period of time, which makes clinical studies on the relative effects of these two dietary variables on cancer risk biomarkers readily feasible.

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Breast Cancer

Cited by 2 publications

References 23 publications

Discrimination of circulating tumor cells of breast cancer and colorectal cancer from normal human mononuclear cells using Raman spectroscopy

Discrimination of circulating tumor cells of breast cancer and colorectal cancer from normal human mononuclear cells using Raman spectroscopy

A Clinical Trial to Selectively Change Dietary Fat and/or Energy Intake in Women: The Women's Diet Study

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