Breakage in Human Chromosomes Induced by Low Temperature

Hampel, K. E.; Levan, Albert

doi:10.1111/j.1601-5223.1964.tb01939.x

Cited by 56 publications

(6 citation statements)

References 17 publications

(2 reference statements)

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“…The stemline numbers recorded for the five cell lines are within the range of chromosome numbers reported for these cells (HAMPEL and LEVAN 1964;MILLER et al 1971;CZAKER 1973;FRANCKE et al 1973;SINHA and PATHAK 1973;WALKER 1973;LIN and GOLDSTEIN 1974;BENGTSON et al 1975;LAVAPPA et al 1976). All five cell lines were near-triploid, D98/AH-2 on the hypotriploid level whereas the other four cell lines within a narrow range on the hypertriploid level.…”

Section: Chromosome Number and Total Relative Lengthsupporting

confidence: 73%

HeLa cells and their possible contamination of other cell lines: Karyotype studies

Heneen¹

2009

Hereditas

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G‐ and C‐banding techniques were applied to the chromosomes of 5 human cell lines, viz. HeLa, KB, HEp‐2, D98/AH‐2 and Lu106. According to the records, the 3 former ones are of tumor origin. the 2 latter of normal origin. The 5 cell lines showed considerable similarities: (1) their stemline chromosome numbers were near‐triploid; (2) the normal chromosome types. generally present in disomic or trisomic numbers, showed the same deviations through 4 or all 5 lines: nulli‐ or monosomy in No. 3, tri‐ or tetrasomy in No. 7, 15 and 17, maximum variation in No. 7, 9 and 16, no Y chromosome in spite of the supposedly male origin of some of the lines; (3) the incidence of markers was of comparable size order (21–25% in KB, HeLa and D98/AH‐2, 29% in HEp‐2, 36% in Lu106); (4) the majority of the marker types were found either in relatively low or high frequencies (1–3 or 8–10 out of 10 cells); (5) marker chromosomes of concordant band pattern were common to different lines; (6) the total lengths of normal and marker chromosomes and whole complements were approximately the same in most or all lines. These results indicate that the variability in the parameters analyzed is less than expected in completely independent lines. The conclusion is that all 5 cell lines are most likely of HeLa origin.

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Section: Chromosome Number and Total Relative Lengthsupporting

confidence: 73%

HeLa cells and their possible contamination of other cell lines: Karyotype studies

Heneen¹

2009

Hereditas

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“…Cold exposure with subsequent warming should produce anomalies of nuclei and chromosomes (Bucciante 1929, Hampel & Levan 1964, Barasa & Tizzani 1965. Obviously, a study of the influence of an analogous treatment on the formation of RS nuclei was desirable.…”

Section: The Effect Of Short-term Cold Exposure Followed By Gradual mentioning

confidence: 99%

The Influence of Temperature on The Formation of Radial‐Segmented Nuclei in Human Lymphocytes and Monocytes

Norberg

1968

Scandinavian Journal of Haematology

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The influence of temperature on the in vitro formation of radial‐segmented (RS) nuclei in lymphocytes and monocytes from peripheral blood was studied quantitatively. The RS nuclei were found to be most abundant after incubation at room temperature, independent of the anticoagulant used. The smallest yield of RS nuclei was found after incubation at refrigerator temperature, a somewhat higher yield after incubation at 37° C. These findings are discussed in relation to the hypothesis that the formation of RS nuclei is due to a contraction of fibrous structures radiating from the centrosomal region, presumably interphasic remnants of the mitotic spindle.

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“…Structural chromosome aberrations were formerly concluded as an accompanying feature of chromosome breaks induced by different agents and cold shock (Hampel and Levan 1964). In the present study, such aberrations were noted as fragmentation, constrictions, gaps and breaks, and might be due to cell death through the apoptotic pathway; also, the striking stickiness of metaphase chromosomes might be one of the reasons for the low frequency of breaks (Eastman and Barry 1992, Yi et al 2007, Yi and Lee 2011.…”

Section: Discussionmentioning

confidence: 84%

“…As previously suggested by Rieder and Cole (2002), this may originate from delays of the cell cycle. Moreover, the pattern of mitotic index also revealed the prospective arrest of cell cycle in M phase with the decrease in temperature, as evidenced by the gradual increase in mitotic index at 4, 0 and -5°C in comparison with the MI at 10°C (Hampel andLevan 1964, Rieder 1981).…”

Section: Discussionmentioning

confidence: 95%

Evaluation of cold shock-induced cytotoxicity and genotoxicity in the house fly Musca domestica

Mishra¹,

Tewari²

2014

Eurasia J Biosci

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Background: Low temperature affects the survival, growth and development of invertebrates, especially insects, based on the severity of cold and the duration of exposure. Although the effects of cold shock or direct chilling were previously analysed in terms of development patterns and defects, morphological changes, cold hardiness, cryopreservation and diapause in insects, very little information is available regarding the effects of cold shock at the chromosomal level. Material and Methods: Late third instar larvae of the house fly Musca domestica were exposed to low temperatures (10, 4, 0 and -5°C) for different durations, in order to assess genotoxicity and cytotoxicity in the present study. The chromosomal aberration assay and micronucleus test were used as genotoxic end points. Cytotoxicity was evaluated by the mitotic index and the extent of tissue damage was observed using the Trypan blue staining method. Results: A significant (P<0.05, P<0.01 and P<0.001) increase in chromosome aberrations and micronucleus frequency was observed in all of the exposed groups compared to the control. The mitotic index showed a dose-dependent increase; however, it was lower in comparison to the control. The developmental patterns in exposed larvae exhibited an increase in larval mortality and a delay in adult emergence. Extensive tissue damage was observed at -5°C by Trypan blue staining. Conclusions: The present work suggests that cold shock induces chromosome aberrations and cytotoxicity and affects the developmental pattern in house fly, M. domestica.

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Breakage in Human Chromosomes Induced by Low Temperature

Cited by 56 publications

References 17 publications

HeLa cells and their possible contamination of other cell lines: Karyotype studies

HeLa cells and their possible contamination of other cell lines: Karyotype studies

The Influence of Temperature on The Formation of Radial‐Segmented Nuclei in Human Lymphocytes and Monocytes

Evaluation of cold shock-induced cytotoxicity and genotoxicity in the house fly Musca domestica

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