Evaluation of cold shock-induced cytotoxicity and genotoxicity in the house fly Musca domestica

Mishra, Nidhi; Tewari, Raghav Ram

doi:10.5053/ejobios.2014.8.0.3

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…(Muldrew et al, 2004;Overgaard and MacMillan, 2017;Overgaard et al, 2005). In M. domestica, cold stress was reported to lead to an increase in chromosome aberrations and micronucleus frequency occurrence (Mishra and Tewari, 2014). Until now, whether cold and freezing stress can damage DNA had not yet been examined in any insect models.…”

Section: Discussionmentioning

confidence: 99%

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Thermal stress causes DNA damage and mortality in a tropical insect

Lubawy

Daburon

Chowański

et al. 2019

Journal of Experimental Biology

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Cold tolerance is considered an important factor determining the geographic distribution of insects. We have previously shown that despite its tropical origin, the cockroach Gromphadorinha coquereliana is capable of surviving exposures to cold. However, the freezing tolerance of this species had not yet been examined. Low temperature is known to alter membrane integrity in insects, but whether chilling or freezing compromises DNA integrity remains a matter of speculation. In the present study, we subjected the G. coquereliana adults to freezing to determine their supercooling point (SCP) and evaluated whether the cockroaches were capable of surviving partial and complete freezing. Next, we conducted single cell gel electrophoresis (SCGE) assays to determine whether heat, cold and freezing altered hemocyte DNA integrity. The SCP of this species was high and around −4.76°C, which is within the typical range of freezing-tolerant species. Most cockroaches survived to 1 day after partial ice formation (20% mortality), but died progressively in the next few days after cold stress (70% mortality after 4 days). One day after complete freezing, most insects died (70% mortality), and after 4 days, 90% of them had succumbed. The SCGE assays showed substantial levels of DNA damage in hemocytes. When cockroaches were heat-stressed, the level of DNA damage was similar to that observed in the freezing treatment, though all heat-stressed insects survived. The present study shows that G. coquereliana can be considered as moderately freeze-tolerant, and that extreme low temperature stress can affect DNA integrity, suggesting that this cockroach may possess an efficient DNA repair system.

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Section: Discussionmentioning

confidence: 99%

“…micronucleus) and chromosomal aberrations (e.g. stickiness, fragmentation or constrictions) (Mishra and Tewari, 2014). Upregulation of several transcripts in cold-stressed bees (e.g.…”

Section: Introductionmentioning

confidence: 99%

Thermal stress causes DNA damage and mortality in a tropical insect

Lubawy

Daburon

Chowański

et al. 2019

Journal of Experimental Biology

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“…micronucleus) and chromosomal aberrations (e.g. stickiness, fragmentation or constrictions) (Mishra and Tewari, 2014). Upregulation of several transcripts in cold-stressed (CLT) bees (e.g.…”

Section: Cytoskeleton and Dna Integritymentioning

confidence: 99%

Mechanisms underpinning the beneficial effects of fluctuating thermal regimes in insect cold tolerance

Colinet

Rinehart

Yocum

et al. 2018

Journal of Experimental Biology

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Insects exposed to low temperature often have high mortality or exhibit sublethal effects. A growing number of recent studies have shown beneficial effects of exposing insects to recurrent brief warm pulses during low-temperature stress (fluctuating thermal regime, FTR). The physiological underpinnings of the beneficial effects of FTR on cold survival have been extensively studied over the past few years. Profiling with various '-omics' techniques has provided supporting evidence for different physiological responses between insects exposed to FTR and constant low temperature. Evidence from transcriptomic, metabolomic and lipidomic studies points to a system-wide loss of homeostasis at low temperature that can be counterbalanced by repair mechanisms under FTR. Although there has been considerable progress in understanding the physiological mechanisms underlying the beneficial effects of FTR, here we discuss how many areas still lack clarity, such as the precise role(s) of heat shock proteins, compatible solutes or the identification of regulators and key players involved in the observed homeostatic responses. FTR can be particularly beneficial in applied settings, such as for model insects used in research, integrated pest management and pollination services. We also explain how the application of FTR techniques in large-scale facilities may require overcoming some logistical and technical constraints. FTR definitively enhances survival at low temperature in insects, but before it can be widely used, we suggest that the possible fitness and energy costs of FTR must be explored more thoroughly. Although FTR is not ecologically relevant, similar processes may operate in settings where temperatures fluctuate naturally.

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