Break monopoly of polarization: CD301b+ macrophages play positive roles in osteoinduction of calcium phosphate ceramics

Wang, Jiaolong; Zhao, Qin; Zheng, Shihang; Wang, Jinyang; Fu, Liangliang; Wang, Can; Zhao, Zifan; Zhang, Yufeng

doi:10.1016/j.apmt.2021.101111

Cited by 7 publications

(14 citation statements)

References 44 publications

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“…The BCP bioceramics with a hydroxyapatite/β-tricalcium phosphate ratio of 60/40 were synthesized as previous protocols [ 17 , 30 ]. In brief, the BCP precursor was made by a chemical precipitation method.…”

Section: Methodsmentioning

confidence: 99%

“…To date, macrophage phenotypes with regenerative regulation, rather than inflammation modulation are still scant. Interestingly, our previous study found the regenerative regulation capacity of CD301b + macrophages during bone formation [ 17 ], which may be promising in the regeneration field. It is reported that CD301b + macrophages are widely infiltrated around the osteoinductive biomaterials [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, our previous study found the regenerative regulation capacity of CD301b + macrophages during bone formation [ 17 ], which may be promising in the regeneration field. It is reported that CD301b + macrophages are widely infiltrated around the osteoinductive biomaterials [ 17 ]. They can promote osteogenic differentiation of MSCs via the IGF1R/Akt/mTOR pathway.…”

Section: Introductionmentioning

confidence: 99%

“…They can promote osteogenic differentiation of MSCs via the IGF1R/Akt/mTOR pathway. Moreover, a few CD301b + macrophages can greatly enhance the osteoinduction of biphasic calcium phosphate (BCP) bioceramics after transplantation in vivo [ 17 ]. On the contrary, the loss of CD301b + macrophages causes the failure of osteoinduction and thus new bone formation [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, a few CD301b + macrophages can greatly enhance the osteoinduction of biphasic calcium phosphate (BCP) bioceramics after transplantation in vivo [ 17 ]. On the contrary, the loss of CD301b + macrophages causes the failure of osteoinduction and thus new bone formation [ 17 ]. Hence, CD301b can be considered a potential marker for regeneration.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

CD301b+ macrophages mediate angiogenesis of calcium phosphate bioceramics by CaN/NFATc1/VEGF axis

Wang

Zhao

et al. 2022

Bioactive Materials

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

CD301b+ macrophages mediate angiogenesis of calcium phosphate bioceramics by CaN/NFATc1/VEGF axis

Wang

Zhao

et al. 2022

Bioactive Materials

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Biphasic calcium phosphate recruits Tregs to promote bone regeneration

Li,

Xia,

Zhao

et al. 2024

Acta Biomaterialia

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Tim4 deficiency reduces CD301b+ macrophage and aggravates periodontitis bone loss

Wang,

Zeng,

Wang

et al. 2024

Int J Oral Sci

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Periodontitis is a common chronic inflammatory disease that causes the periodontal bone destruction and may ultimately result in tooth loss. With the progression of periodontitis, the osteoimmunology microenvironment in periodontitis is damaged and leads to the formation of pathological alveolar bone resorption. CD301b+ macrophages are specific to the osteoimmunology microenvironment, and are emerging as vital booster for conducting bone regeneration. However, the key upstream targets of CD301b+ macrophages and their potential mechanism in periodontitis remain elusive. In this study, we concentrated on the role of Tim4, a latent upstream regulator of CD301b+ macrophages. We first demonstrated that the transcription level of Timd4 (gene name of Tim4) in CD301b+ macrophages was significantly upregulated compared to CD301b− macrophages via high-throughput RNA sequencing. Moreover, several Tim4-related functions such as apoptotic cell clearance, phagocytosis and engulfment were positively regulated by CD301b+ macrophages. The single-cell RNA sequencing analysis subsequently discovered that Cd301b and Timd4 were specifically co-expressed in macrophages. The following flow cytometric analysis indicated that Tim4 positive expression rates in total macrophages shared highly synchronized dynamic changes with the proportions of CD301b+ macrophages as periodontitis progressed. Furthermore, the deficiency of Tim4 in mice decreased CD301b+ macrophages and eventually magnified alveolar bone resorption in periodontitis. Additionally, Tim4 controlled the p38 MAPK signaling pathway to ultimately mediate CD301b+ macrophages phenotype. In a word, Tim4 might regulate CD301b+ macrophages through p38 MAPK signaling pathway in periodontitis, which provided new insights into periodontitis immunoregulation as well as help to develop innovative therapeutic targets and treatment strategies for periodontitis.

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Break monopoly of polarization: CD301b+ macrophages play positive roles in osteoinduction of calcium phosphate ceramics

Cited by 7 publications

References 44 publications

CD301b+ macrophages mediate angiogenesis of calcium phosphate bioceramics by CaN/NFATc1/VEGF axis

CD301b+ macrophages mediate angiogenesis of calcium phosphate bioceramics by CaN/NFATc1/VEGF axis

Biphasic calcium phosphate recruits Tregs to promote bone regeneration

Tim4 deficiency reduces CD301b+ macrophage and aggravates periodontitis bone loss

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