CD301b+ macrophages mediate angiogenesis of calcium phosphate bioceramics by CaN/NFATc1/VEGF axis

Wang, Jiaolong; Zhao, Qin; Fu, Liangliang; Zheng, Shihang; Wang, Can; Han, Litian; Gong, Zijian; Wang, Ziming; Tang, Hua; Zhang, Yufeng

doi:10.1016/j.bioactmat.2022.02.004

Cited by 17 publications

(17 citation statements)

References 63 publications

(108 reference statements)

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“…In this regard, several studies have investigated the interaction between immune cells and biomaterials/osteogenic cells. The RAW 264.7 murine macrophage cell line [ 14 , 59 , 75 , 81 , 86 , 88 , 102 , 108 , 137 , 138 , 139 ], macrophages derived from the human monocyte THP-1 line [ 76 ], or primary bone marrow-derived monocytes [ 74 , 140 ], are alternatively exploited as suitable cellular models to study biomaterial-induced inflammatory responses. In addition, specific protocols are used to test immune cell adhesion, viability, and growth.…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

“…The vascularization of the implanted bone substitute is indispensable for appropriate bone healing since vessels provide the main source of nutrients for the entire bone niche. The human umbilical vein endothelial (HUVECs) cell line is commonly used to assess the effects of bone biomaterial on vessel formation in vitro [ 75 , 105 , 139 , 140 , 142 , 143 , 144 , 145 ]. As an alternative, the use of human aortic endothelial cells (HAECs) is also reported [ 146 ].…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

“…As an alternative, the use of human aortic endothelial cells (HAECs) is also reported [ 146 ]. The tube formation assay is a classic angiogenesis assay that can be used to assess the angiogenic differentiation of HUVECs in contact with a specific biomaterial; the tube structures as well as the total branch length can be analyzed with a contrast inverted microscope [ 86 , 105 , 140 , 142 , 143 ].…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

“…The expression of angiogenic related genes, such as platelet and endothelial cell adhesion molecule 1 (PECAM1/CD31), vascular endothelial growth factor A (VEGFA), fibroblast growth factor 2 (FGF2), kinase insert domain receptor/vascular endothelial growth factor receptor 2 (KDR), C-X-C motif chemokine ligand 12 (CXCL12/SDF1A), nitric oxide synthase 3 (NOS3 ), and hypoxia inducible factor 1 subunit alpha (HIF1A) can be analyzed by RT-qPCR and/or immunofluorescence and/or Western blot analysis and/or ELISA [ 75 , 86 , 105 , 107 , 139 , 140 , 142 , 143 , 144 , 145 ].…”

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

See 3 more Smart Citations

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Pietro

Palmieri

Papi

et al. 2022

IJMS

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In the last 20 years, bone regenerative research has experienced exponential growth thanks to the discovery of new nanomaterials and improved manufacturing technologies that have emerged in the biomedical field. This revolution demands standardization of methods employed for biomaterials characterization in order to achieve comparable, interoperable, and reproducible results. The exploited methods for characterization span from biophysics and biochemical techniques, including microscopy and spectroscopy, functional assays for biological properties, and molecular profiling. This review aims to provide scholars with a rapid handbook collecting multidisciplinary methods for bone substitute R&D and validation, getting sources from an up-to-date and comprehensive examination of the scientific landscape.

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Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

Section: Other Biomaterials Bioactivitiesmentioning

confidence: 99%

See 2 more Smart Citations

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Pietro

Palmieri

Papi

et al. 2022

IJMS

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“…Therefore, these cells may play a more comprehensive role than the traditional M2 macrophages by being involved in both in ammatory and regenerative repair processes 19,20 . Previous studies have demonstrated that CD301b+ macrophages are necessary not only for tissue regeneration 18 , but also for bone defect repair and regeneration 21,22 . Therefore, it is suggested that these cells may be considered as targets for orthodontic treatment in the in ammatory environment.…”

Section: Introductionmentioning

confidence: 99%

CD301b+ macrophages: a new target for improving the efficiency of orthodontic treatment under mild inflammation

Wang

Zhao

et al. 2022

Preprint

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With improvements in the quality of life and the demand for aesthetics, more and more people are opting for orthodontic treatment, resulting in a surge in adult orthodontic patients in recent years. However, a large body of clinical evidence shows that many orthodontic patients experience periodontal inflammation, which hinders the effect of the treatment or exacerbates the periodontal status. Therefore, determining the key factors that affect orthodontic treatment in an inflammatory environment to improve the efficiency of the treatment is vital. In this study, we focused on the critical role of macrophages during orthodontic treatment in an inflammatory environment. The functional population of macrophages was analyzed in a rat orthodontic model with periodontitis. Macrophages with high expression levels of CD301b improved the periodontal microenvironment and the efficiency of the orthodontic tooth movement. Transplantation of CD301b + macrophages into the model promoted osteogenesis around the orthodontic mobile teeth, improved bone remodeling during the orthodontic process, and accelerated orthodontic tooth movement. These findings indicate that CD301b + macrophages may have a positive effect during orthodontic treatment in an inflammatory environment and could be considered as a potential regulatory target.

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Fn‐HMGB1 Adsorption Behavior Initiates Early Immune Recognition and Subsequent Osteoinduction of Biomaterials

Zhao,

Zhang

et al. 2023

Adv Healthcare Materials

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Implantable biomaterials are widely used in bone tissue engineering, but little is still known about how they initiate early immune recognition and the initial dynamics. Herein, the early immune recognition and subsequent osteoinduction of biphasic calcium phosphate (BCP) after implantation to the protein adsorption behavior is attributed. By liquid chromatography tandem mass spectrometry (LC‒MS/MS) analysis, the biomaterial‐related molecular patterns (BAMPs) formed after BCP implantation are mapped, dominated by the highly expressed extracellular matrix protein fibronectin (Fn) and the high mobility group box 1 (HMGB1). Molecular dynamics simulations show that Fn has the ability to bind more readily to the BCP surface than HMGB1. The preferential binding of Fn provides a higher adsorption energy for HMGB1. Furthermore, multiple hydrogen bonding sites between HMGB1 and Fn are demonstrated using a molecular docking approach. Ultimately, the formation of BAMPs through HMGB1 antagonist glycyrrhizic acid (GA), resulting in impaired immune recognition of myeloid differentiation factor 88 (MYD88) mediated dendritic cells (DCs) and macrophages (Mφs), as well as failed osteoinduction processes is obstructed. This study introduces a mechanism for early immune recognition of implant materials based on protein adsorption, providing perspectives for future design and application of tissue engineering materials.

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CD301b+ macrophages mediate angiogenesis of calcium phosphate bioceramics by CaN/NFATc1/VEGF axis

Cited by 17 publications

References 63 publications

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

Translating Material Science into Bone Regenerative Medicine Applications: State-of-The Art Methods and Protocols

CD301b+ macrophages: a new target for improving the efficiency of orthodontic treatment under mild inflammation

Fn‐HMGB1 Adsorption Behavior Initiates Early Immune Recognition and Subsequent Osteoinduction of Biomaterials

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