Bread wheat with enhanced grain carotenoid content: a novel option for wheat biofortification

Padhy, Asish Kumar; Sharma, Achla; Sharma, Himanshu; Rajput, Ruchika; Pandey, Ashutosh; Srivastava, Puja; Kaur, Satinder; Kaur, Harinderjit; Singh, Surjan; Kashyap, Lenika; Mavi, G. S.; Kaur, Jaspal; Sohu, V. S.; Chhuneja, Parveen; Bains, N. S.

doi:10.1007/s11032-022-01338-0

Cited by 5 publications

(4 citation statements)

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“…They also reported a negative correlation between carotenoid content and grain width and grain thickness. Similarly, Padhy A. K. et al (2022) reported a significant negative correlation between carotenoid content and thousand-grain weight and spike length. Spikelets per spike and grains per spike were negatively correlated with carotenoid content but were insignificant.…”

Section: Discussionmentioning

confidence: 88%

“…The incorporation of the PsyE1 gene increases all the carotenoids that are produced in the carotenoid biosynthesis pathway. There is an increase in lutein, zeaxanthin, and β-carotein content due to the introgression of PsyE1 ( Zhang et al, 2005 ; Ceoloni et al, 2017 ; Padhy A. K. et al, 2022 ). In a bread wheat background, despite the numerous health benefits of carotenoids, the gene was never used commercially as the yellow coloration of wheat flour lacked consumer acceptability and health-associated awareness.…”

Section: Discussionmentioning

confidence: 99%

“…First set of germplasm consisted of BC 1 F 5 bulk plants having PsyE1 ( Y gene) in HD2967 background (HD2967 + PsyE1/Sr25/Lr19 ). The set consisted of 180 lines, out of which an agronomically better-performing line with high pigment content was chosen and used as parent 1 ( Padhy et al, 2022c ). A commercial bread wheat variety HD2967 (ALD/COC//URES/3/HD2160 M/HD2278) developed by the Indian Agricultural Research Institute (IARI), New Delhi for cultivation under timely sown irrigated conditions in the northwestern plain zone of India (notification number 2326 (E) dated 10.10.2011, Indian Council of Agricultural Research (ICAR), Government of India).…”

Section: Methodsmentioning

confidence: 99%

“…elongatom and Th. ponticum ) have been reported to contain a high amount of carotenoids with a range of 5.3–13.6 ppm ( Abdel-Aal et al, 2002 ; Padhy et al, 2022a ; Padhy A. K. et al, 2022 ), thereby opening avenues for carotenoid biofortification in bread wheat. These genes, therefore, if pyramided with other nutritional and agriculturally important attributes, could potentially translate wheat into a complete staple food with higher nutritive value and health benefits.…”

Section: Introductionmentioning

confidence: 99%

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Combining high carotenoid, grain protein content and rust resistance in wheat for food and nutritional security

Padhy

Sharma²,

Sharma³

et al. 2023

Front. Genet.

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Globally, malnutrition has given birth to an alarming predicament, especially in developing countries, and has extensively shifted consumer preferences from conventional high-energy diets to a nutritionally balanced, cost-effective, sustainable, and healthy lifestyle. In keeping with this view and the mandate for developing high-yielding, disease-resistant biofortified staple food (wheat) for catering to the demand-driven market, the current research aimed at stacking together the enhanced grain protein content, carotenoid content, and disease resistance in an elite bread wheat background. The Y gene (PsyE1) and the GpcB1 gene were used as novel sources for enhancing the grain carotenoid and protein content in the commercial elite bread wheat cultivar HD2967. The combination also led to the stacking of resistance against all three foliar rusts owing to linked resistance genes. A stepwise hybridization using Parent 1 (HD2967 + PsyE1/Lr19/Sr25) with Parent 2 (PBW550 + GpcB1/Yr36+ Yr15), coupled with a phenotypic-biochemical selection, narrowed down 2748 F2 individuals to a subset of 649 F2 plants for molecular screening. The gene-specific markers PsyE1, PsyD1, Xucw108, and Xbarc8 for the genes PsyE1, PsyD1, GpcB1, and Yr15, respectively, were employed for forward selection. Four bread wheat lines positive for all the desired genes with high carotenoid (>8ppm) and protein (>13%) content were raised to the F5 generation and will be evaluated for yield potential after bulking. These improved advanced breeding lines developed following multipronged efforts should prove a valuable and unique source for the development of cultivars with improved nutritional quality and rust resistance in wheat breeding programs.

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Section: Discussionmentioning

confidence: 88%