BRD7 plays an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway

Abstract

Increasing evidence has shown a strong association between tumor-suppressor genes and inflammation. However, the role of BRD7 as a novel tumor suppressor in inflammation remains unknown. In this study, by observing BRD7 knockout mice for 6-12 months, we discovered that compared with BRD7 mice, BRD7 mice were more prone to inflammation, such as external inflammation and abdominal abscess. By using mouse embryo fibroblast (MEF) cells from the BRD7 knockout mouse, an in vitro lipopolysaccharide (LPS)-stimulated MEF cell line was established. The mRNA levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), chemokine (C-X-C motif) ligand 1 (CXCL-1) and inducible nitric oxide synthase (iNOS) were significantly increased in BRD7 MEF cells compared with BRD7 MEF cells after LPS stimulation for 1 or 6 h. In addition, the cytoplasm-to-nucleus translocation of nuclear factor kappa-B (NF-κB; p65) and an increased NF-κB reporter activity were observed in BRD7 MEF cells at the 1 h time point but not at the 6 h time point. Furthermore, an in vivo dextran sodium sulfate (DSS)-induced acute colitis model was created. As expected, the disease activity index (DAI) value was significantly increased in the BRD7 mice after DSS treatment for 1-5 days, which was demonstrated by the presence of a significantly shorter colon, splenomegaly and tissue damage. Moreover, higher expression levels of IL-6, TNF-α, p65, CXCL-1 and iNOS, and an increased level of NF-κB (p65) nuclear translocation were also found in the DSS-treated BRD7 mice. These findings suggest that BRD7 has an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway, which provides evidence to aid in understanding the therapeutic effects of BRD7 on inflammatory diseases.

Upregulated long non-coding RNA LINC00152 expression is associated with progression and poor prognosis of tongue squamous cell carcinoma

“…Total RNA was extracted from frozen tissues using an RNeasy Mini Kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA according to the manufacturer's instructions 36-42. One μg of total RNA was reverse transcribed to cDNA using a Reverse Transcription Kit (Biorad, Hercules, CA, USA).…”

High Expression of LINC01420 indicates an unfavorable prognosis and modulates cell migration and invasion in nasopharyngeal carcinoma

“…Human nasopharyngeal epithelium cell line NP69 and nasopharyngeal carcinoma HNE1, HK-1, HNE2 and 5-8F cell lines were maintained in our laboratory 27-33. Cell were maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS, Invitrogen, Shanghai, China) in a humidified incubator with 5% CO 2 at 37 ℃.…”

Overexpression long non-coding RNA LINC00673 is associated with poor prognosis and promotes invasion and metastasis in tongue squamous cell carcinoma

“…Cells on the upper surface were wiped by a cotton bud. Numbers of invasive cells were counted from six randomly selected 200× fields in under a microscope and shown as the average per field [66–68]. …”