Brazilian red propolis: Chemical composition and antibacterial activity determined using bioguided fractionation

Rufatto, Luciane Corbellini; Luchtenberg, Paola; Garcia, Charlene Silvestrin Celi; Thomassigny, Christine; Bouttier, Sylvie; Henriques, João Antônio Pêgas; Roesch‐Ely, Mariana; Dumas, Françoise; Moura, Sidnei

doi:10.1016/j.micres.2018.05.003

Cited by 81 publications

(62 citation statements)

References 43 publications

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“…It is well documented that the total content of phenolic compounds present in propolis varies according to several factors, including the extraction method and conditions used [ 57 ]. Regarding red propolis, previously studies have reported the presence of more than 300 components, which are representatives of terpenes, flavonoids, aromatic acids and fatty acids [ 22 ]. Flavonoids, are the most common and widely distributed group of phenolics in red propolis, being the most active compound in this natural matrix, with formononetin as the most relevant chemical marker of this type of propolis [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have also reported higher concentrations of phenolic compounds, such as formononetin, in extracts of red propolis, which is identified as its major component [ 4 , 15 , 22 ]. López et al [ 64 ], when evaluating samples of red propolis from different regions by mass spectrometry, identified the presence of formononetin ( m / z 267.06, retention time, rt 4.5 min) in all analyzed samples.…”

Section: Resultsmentioning

confidence: 99%

“…Given this context, research has intensified on different extracts from natural matrices that have a wide variety of compounds with beneficial effects on health, such as propolis [ 16 , 17 , 18 , 19 ]. Among the various types of propolis, classified according to their physicochemical properties and geographical location, red propolis has gained prominence due to its composition and pharmacological properties [ 20 , 21 , 22 ]. Originating in Northeast Brazil, red propolis has biologically active flavonoids as its major compounds and may also contain cinnamic acid derivatives, esters and some terpenes [ 14 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among the various types of propolis, classified according to their physicochemical properties and geographical location, red propolis has gained prominence due to its composition and pharmacological properties [ 20 , 21 , 22 ]. Originating in Northeast Brazil, red propolis has biologically active flavonoids as its major compounds and may also contain cinnamic acid derivatives, esters and some terpenes [ 14 , 22 , 23 ]. Flavonoids are the most common and most varied group among the phenolic compounds of red propolis, play a role in different physiological processes and perform different functions, including beehive protection [ 13 , 14 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Originating in Northeast Brazil, red propolis has biologically active flavonoids as its major compounds and may also contain cinnamic acid derivatives, esters and some terpenes [ 14 , 22 , 23 ]. Flavonoids are the most common and most varied group among the phenolic compounds of red propolis, play a role in different physiological processes and perform different functions, including beehive protection [ 13 , 14 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Supercritical Extraction of Red Propolis: Operational Conditions and Chemical Characterization

et al. 2020

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The objective of this study was to determine the best operational conditions for obtaining red propolis extract with high antioxidant potential through supercritical fluid extraction (SFE) technology, using carbon dioxide (CO2) as the supercritical fluid and ethanol as the cosolvent. The following parameters were studied: overall extraction curve, S/F (mass of CO2/mass of sample), cosolvent percentage (0, 1, 2 and 4%) and global yield isotherms as a function of different pressures (250, 350 and 450 bar) and temperatures (31.7, 40 and 50 °C). Within the investigated parameters, the best conditions found were an S/F of 131 and the use of ethanol at the highest concentration (4% w/w), which resulted in higher extract yields and higher content of antioxidant compounds. Formononetin, the main biomarker of red propolis, was the compound found at the highest amounts in the extracts. As expected, the temperature and pressure conditions also influenced the process yield, with 350 bar and 40 °C being the best conditions for obtaining bioactive compounds from a sample of red propolis. The novel results for red propolis found in this study show that it is possible to obtain extracts with high antioxidant potential using a clean technology under the defined conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Supercritical Extraction of Red Propolis: Operational Conditions and Chemical Characterization

et al. 2020

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Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Silva

Braun

et al. 2021

Chemistry & Biodiversity

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Brazilian green and red propolis stand out as commercial products for different medical applications. In this article, we report the antimicrobial activities of the hydroalcoholic extracts of green (EGP) and red (ERP) propolis, as well as guttiferone E plus xanthochymol (8) and oblongifolin B (9) from red propolis, against multidrug‐resistant bacteria (MDRB). We undertook the minimal inhibitory (MIC) and bactericidal (MBC) concentrations, inhibition of biofilm formation (MICB50), catalase, coagulase, DNase, lipase, and hemolysin assays, along with molecular docking simulations. ERP was more effective by displaying MIC and MBC values <100 μg mL−1. Compounds 8 and 9 displayed the lowest MIC values (0.98 to 31.25 μg mL−1) against all tested Gram‐positive MDRB. They also inhibited the biofilm formation of S. aureus (ATCC 43300 and clinical isolate) and S. epidermidis (ATCC 14990 and clinical isolate), with MICB50 values between 1.56 and 6.25 μg mL−1. The molecular docking results indicated that 8 and 9 might interact with the catalase's amino acids. Compounds 8 and 9 have great antimicrobial potential.

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Antiparasitic Properties of Propolis Extracts and Their Compounds

Paula

Cândido

Santos

et al. 2021

Chemistry & Biodiversity

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Propolis is a bee product that has been used in medicine since ancient times. Although its anti‐inflammatory, antioxidant, antimicrobial, antitumor, and immunomodulatory activities have been investigated, its anti‐parasitic properties remain poorly explored, especially regarding helminths. This review surveys the results obtained with propolis around the world against human parasites. Regarding protozoa, studies carried out with the protozoa Trypanosoma spp. and Leishmania spp. have demonstrated promising results in vitro and in vivo. However, there are fewer studies for Plasmodium spp., the etiological agent of malaria and less so for helminths, particularly for Fasciola spp. and Schistosoma spp. Despite the favorable in vitro results with propolis, helminth assays need to be further investigated. However, propolis has shown itself to be an excellent natural product for parasitology, thus opening new paths and approaches in its activity against protozoa and helminths.

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Brazilian red propolis: Chemical composition and antibacterial activity determined using bioguided fractionation

Cited by 81 publications

References 43 publications

Supercritical Extraction of Red Propolis: Operational Conditions and Chemical Characterization

Supercritical Extraction of Red Propolis: Operational Conditions and Chemical Characterization

Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Antiparasitic Properties of Propolis Extracts and Their Compounds

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