Braveheart, a Long Noncoding RNA Required for Cardiovascular Lineage Commitment

Klattenhoff, Carla Andrea; Scheuermann, Johanna C.; Surface, Lauren E.; Bradley, Robert K.; Fields, Paul A.; Steinhauser, Matthew L.; Ding, Huiming; Butty, Vincent; Torrey, Lillian; Haas, Simon; Abo, Ryan; Tabebordbar, Mohammadsharif; Lee, Richard T.; Burge, Christopher B.; Boyer, Laurie A.

doi:10.1016/j.cell.2013.01.003

Cited by 836 publications

(725 citation statements)

References 82 publications

Supporting

Mentioning

706

Contrasting

Unclassified

Order By: Relevance

“…LncRNAs, a class of non‐coding RNAs with a length greater than 200 nt, can be involved in genetic regulation transcriptionally and post‐transcriptionally, and some of them, including Bvrt, Fendrr, ANRIL, MIAT, MyHeart (Mhrt), functioned essentially in the occurrence of heart diseases, including myocardial infarction, cardiomyopathy, heart failure and atherosclerosis 23, 24, 25, 26, 27, 28. ANRIL was a 3.8‐kb‐long non‐coding RNA transcribed from chromosome 9p21, which was a crucial locus of genetic sensitivity for CAD 29, 30.…”

Section: Discussionmentioning

confidence: 99%

The interplay of LncRNA ANRIL and miR‐181b on the inflammation‐relevant coronary artery disease through mediating NF‐κB signalling pathway

Guo

Tang

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

This study was designed to investigate whether ANRIL affected the aetiology of coronary artery disease (CAD) by acting on downstream miR‐181b and NF‐κB signalling. Altogether 327 CAD patients diagnosed by angiography were included, and mice models of CAD were established. Human coronary endothelial cells (HCAECs) and human umbilical vein endothelial cells (HUVECs) were also purchased. In addition, shRNA‐ANRIL, shRNA‐NC, pcDNA3.1‐ANRIL, miR‐181b mimic, miR‐181b inhibitor and miR‐NC were transfected into the cells. The lipopolysaccharides (LPS) and pyrrolidine dithiocarbamate (PDTC) were also added to activate or deactivate NF‐κB signalling. Both highly expressed ANRIL and lowly expressed miR‐181b were associated with CAD population aged over 60 years old, with smoking history, with hypertension and hyperlipidemia, with CHOL H 4.34 mmol/L, TG ≥ 1.93 mmol/L and Hcy ≥ 16.8 μmol/L (all P < 0.05). Besides, IL‐6, IL‐8, NF‐κB, TNF‐α, iNOS, ICAM‐1, VCAM‐1 and COX‐2 expressions observed within AD mice models were all beyond those within NC and sham‐operated groups (P < 0.05). Also VEGF and HSP 70 were highly expressed within AD mice models than within NC and sham‐operated mice (P < 0.05). Transfection of either pcDNA‐ANRIL or miR‐181b inhibitor could significantly fortify HCAECs’ viability and put on their survival rate. At the meantime, the inflammatory factors and vascular‐protective parameters were released to a greater level (P < 0.05). Finally, highly expressed ANRIL also notably bring down miR‐181b expression and raise p50/p65 expressions within HCAECs (P < 0.05). The joint role of ANRIL, miR‐181b and NF‐κB signalling could aid in further treating and diagnosing CAD.

show abstract

Section: Discussionmentioning

confidence: 99%

The interplay of LncRNA ANRIL and miR‐181b on the inflammation‐relevant coronary artery disease through mediating NF‐κB signalling pathway

Guo

Tang

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…For example, HI-LNC25, a β cell-specific lncRNA, is dysregulated in type 2 diabetes, due to the down-regulation of the Kruppel-like zinc finger transcription factor (GLIS3) mRNA [19]. Braveheart (Bvht), a heart-associated lncRNA, is required for the progression of nascent mesoderm toward a cardiac fate [20]. One hundred and seventy-five lncRNAs are specifically regulated during adipogenesis [21].…”

Section: Introductionmentioning

confidence: 99%

Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients

Huang

Hao

Bao

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To describe the long noncoding RNA (lncRNA) profiles in cumulus cells isolated from polycystic ovary syndrome (PCOS) patients by employing a microarray and in-depth bioinformatics analysis. This information will help us understand the occurrence and development of PCOS. Methods In this study, we used a microarray to describe lncRNA profiles in cumulus cells isolated from ten patients (five PCOS and five normal women). Several differentially expressed lncRNAs were chosen to validate the microarray results by quantitative RT-PCR (qRT-PCR). Then, the differentially expressed lncRNAs were classified into three subgroups (HOX loci lncRNA, enhancer-like lncRNA, and lincRNA) to deduce their potential features. Furthermore, a lncRNA/mRNA co-expression network was constructed by using the Cytoscape software (V2.8.3, http://www.cytoscape.org/). Results We observed that 623 lncRNAs and 260 messenger RNAs (mRNAs) were significantly up-or down-regulated (≥2-fold change), and these differences could be used to discriminate cumulus cells of PCOS from those of normal pat i e n t s . F i v e d i f f e r e n t i a l l y e x p r e s s e d l n c R N A s (XLOC_011402, ENST00000454271, ENST00000433673, ENST00000450294, and ENST00000432431) were selected to validate the microarray results using quantitative RT-PCR (qRT-PCR). The qRT-PCR results were consistent with the microarray data. Further analysis indicated that many differentially expressed lncRNAs were transcribed from chromosome 2 and may act as enhancers to regulate their neighboring protein-coding genes. Forty-three lncRNAs and 29 mRNAs were used to construct the coding-non-coding gene coexpression network. Most pairs positively correlated, and one mRNA correlated with one or more lncRNAs. Conclusions Our study is the first to determine genome-wide lncRNA expression patterns in cumulus cells isolated from PCOS patients by microarray. The results show that clusters of lncRNAs were aberrantly expressed in cumulus cells of PCOS patients compared with those of normal women, which revealed that lncRNAs differentially expressed in PCOS and normal women may contribute to the occurrence of PCOS and affect oocyte development.

show abstract

“…Braveheart, a novel lncRNA, has been defined to be a critical regulator of cardiovascular commitment from embryonic stem cells (ESCs). 7,8 Mechanistically, braveheart mediates the epigenetic regulation of cardiac commitment by interacting with SUZ12, a component of the polycomb repressive complex 2 (PRC2). 7,9 Fendrr, another novel cardiac lncRNA, has been shown to be an essential regulator of heart and body wall development.…”

Section: Introductionmentioning

confidence: 99%

“…7,8 Mechanistically, braveheart mediates the epigenetic regulation of cardiac commitment by interacting with SUZ12, a component of the polycomb repressive complex 2 (PRC2). 7,9 Fendrr, another novel cardiac lncRNA, has been shown to be an essential regulator of heart and body wall development. 8,10 Most recently, several studies have documented that expression and function of lncRNAs are associated with a variety of cardiovascular conditions.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNAs link extracellular matrix gene expression to ischemic cardiomyopathy

et al. 2016

View full text Add to dashboard Cite

Braveheart, a Long Noncoding RNA Required for Cardiovascular Lineage Commitment

Cited by 836 publications

References 82 publications

The interplay of LncRNA ANRIL and miR‐181b on the inflammation‐relevant coronary artery disease through mediating NF‐κB signalling pathway

The interplay of LncRNA ANRIL and miR‐181b on the inflammation‐relevant coronary artery disease through mediating NF‐κB signalling pathway

Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients

Long non-coding RNAs link extracellular matrix gene expression to ischemic cardiomyopathy

Contact Info

Product

Resources

About