Brassinosteroid regulation of wood formation in poplar

Du, Juan; Gerttula, Suzanne; Li, Zehua; Zhao, Shutang; Liu, Yingli; Liu, Yu; Lu, Mengzhu; Groover, Andrew

doi:10.1111/nph.15936

Cited by 62 publications

(55 citation statements)

References 55 publications

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“…2). Hence, our results indicated that PtoDET2 could significantly increase BRs level for much enhanced plant growth and biomass yield, consistent with the previous findings that BRs-related genes could promote plant growth [31][32][33][34][35][36][37][38][39][40].…”

Section: Ptodet2 Overexpression Improved Plant Growth and Biomass Yiesupporting

confidence: 92%

“…Compared to the WT, the PtoDET2-OE transgenic lines exhibited much expanded xylem area with significantly increased stem diameter and amounts of xylem cell layers as well as larger vessel/fiber cells ( Fig. 3a, b), in agreement with the previous reports that BRs promote differentiation of xylem cells [33][34][35]. Accordingly, we detected significantly increased transcription levels of four representative genes involved in xylem cell differentiation and expansion (Additional file 1: Fig.…”

Section: Ptodet2 Affected Wood Formation and Wall Polysaccharides Biosupporting

confidence: 90%

“…BRs are the plant-specific steroid hormones that dynamically regulate plant growth and development [30][31][32][33][34][35][36]. In this study, we observed a fast growth and development with more internodes and larger leaves in the PtoDET2-OE transgenic lines ( Fig.…”

Section: Ptodet2 Overexpression Improved Plant Growth and Biomass Yiesupporting

confidence: 52%

“…Recently, BRs were shown to take part in regulating wood formation, xylem differentiation, and cell wall biosynthesis [33][34][35][36]. Previous studies have demonstrated that DET2 catalyzes a 5α-reduction of multiple related sterols, and DET2 is an important rate-limiting enzyme in the BR biosynthesis pathway [46,47].…”

Section: Discussionmentioning

confidence: 99%

“…BIN2 controls the stabilization and activation of BRASSINAZOLE-RESISTANT1 (BRZ1) and BR-INSENSITIVE-EMS-SUPPRESSOR 1 (BES1/BZR2), and therefore to regulate the transcription of BR-responsive target genes [31,32]. Overexpression of BR biosynthesis or signaling genes led to more xylem formation and increased cell wall deposition [31][32][33][34][35][36]. Although increasing evidence has established the connection between BRs and wood formation, the role of BRs during cell wall polysaccharide biosynthesis and modification is not well revealed, and the effects of BRs in bioethanol production are largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Fan

Qin

et al. 2020

Biotechnol Biofuels

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Background: As a leading biomass feedstock, poplar plants provide enormous lignocellulose resource convertible for biofuels and bio-chemicals. However, lignocellulose recalcitrance particularly in wood plants, basically causes a costly bioethanol production unacceptable for commercial marketing with potential secondary pollution to the environment. Therefore, it becomes important to reduce lignocellulose recalcitrance by genetic modification of plant cell walls, and meanwhile to establish advanced biomass process technology in woody plants. Brassinosteroids, plantspecific steroid hormones, are considered to participate in plant growth and development for biomass production, but little has been reported about brassinosteroids roles in plant cell wall assembly and modification. In this study, we generated transgenic poplar plant that overexpressed DEETIOLATED2 gene for brassinosteroids overproduction. We then detected cell wall feature alteration and examined biomass enzymatic saccharification for bioethanol production under various chemical pretreatments.Results: Compared with wild type, the PtoDET2 overexpressed transgenic plants contained much higher brassinosteroids levels. The transgenic poplar also exhibited significantly enhanced plant growth rate and biomass yield by increasing xylem development and cell wall polymer deposition. Meanwhile, the transgenic plants showed significantly improved lignocellulose features such as reduced cellulose crystalline index and degree of polymerization values and decreased hemicellulose xylose/arabinose ratio for raised biomass porosity and accessibility, which led to integrated enhancement on biomass enzymatic saccharification and bioethanol yield under various chemical pretreatments. In contrast, the CRISPR/Cas9-generated mutation of PtoDET2 showed significantly lower brassinosteroids level for reduced biomass saccharification and bioethanol yield, compared to the wild type. Notably, the optimal green-like pretreatment could even achieve the highest bioethanol yield by effective lignin extraction in the transgenic plant. Hence, this study proposed a mechanistic model elucidating how brassinosteroid regulates cell © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article' Full list of author information is available at the end of the article wall modification for reduced lignocellulose recalcitrance and increased biomass porosity and accessibility for high bioethanol production. Conclusions:This study has demonstrated a powerful strategy to enhance cell...

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Section: Ptodet2 Overexpression Improved Plant Growth and Biomass Yiesupporting

confidence: 92%

Section: Ptodet2 Affected Wood Formation and Wall Polysaccharides Biosupporting

confidence: 90%

Section: Ptodet2 Overexpression Improved Plant Growth and Biomass Yiesupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Fan

Qin

et al. 2020

Biotechnol Biofuels

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Genetic adaptation of Tibetan poplar (Populus szechuanica var. tibetica) to high altitudes on the Qinghai–Tibetan Plateau

Zheng

Li-zhi

Sang

et al. 2020

Ecology and Evolution

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Plant adaptation to high altitudes has long been a substantial focus of ecological and evolutionary research. However, the genetic mechanisms underlying such adaptation remain poorly understood. Here, we address this issue by sampling, genotyping, and comparing populations of Tibetan poplar, Populus szechuanica var. tibetica , distributed from low (~2,000 m) to high altitudes (~3,000 m) of Sejila Mountain on the Qinghai–Tibet Plateau. Population structure analyses allow clear classification of two groups according to their altitudinal distributions. However, in contrast to the genetic variation within each population, differences between the two populations only explain a small portion of the total genetic variation (3.64%). We identified asymmetrical gene flow from high‐ to low‐altitude populations. Integrating population genomic and landscape genomic analyses, we detected two hotspot regions, one containing four genes associated with altitudinal variation, and the other containing ten genes associated with response to solar radiation. These genes participate in abiotic stress resistance and regulation of reproductive processes. Our results provide insight into the genetic mechanisms underlying high‐altitude adaptation in Tibetan poplar.

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Cassava (Manihot esculenta) dual use for food and bioenergy: A review

Fathima

Sanitha

Tripathi

et al. 2022

Food and Energy Security

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Cassava is an important food crop with an average consumption of 50 kg/capita/year in Africa (FAO, 2018). The global cassava production stands at slightly over 11 tonnes per hectare. Over 63% of the 303 million tonnes produced globally in 2019 was from Africa (FAO, 2019). Despite being the leading region in cassava production, only 3000 tonnes of the produce are dedicated to non-food uses. The challenges associated with global fossil fuels

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Brassinosteroid regulation of wood formation in poplar

Cited by 62 publications

References 55 publications

Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Genetic adaptation of Tibetan poplar (Populus szechuanica var. tibetica) to high altitudes on the Qinghai–Tibetan Plateau

Cassava (Manihot esculenta) dual use for food and bioenergy: A review

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