Brain‐specific 1B promoter of FGF1 gene facilitates the isolation of neural stem/progenitor cells with self‐renewal and multipotent capacities

Hsu, Yi‐Chao; Lee, Don Ching; Chen, Su Liang; Liao, Wei; Lin, Jia Wei; Chiu, Wen Ta; Chiu, Ing Ming

doi:10.1002/dvdy.21753

Cited by 52 publications

(68 citation statements)

References 65 publications

(99 reference statements)

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“…27 We placed the fabricated conduit in a six-well plate and sterilized it for 24 h with ultraviolet light. Then, we injected the cells with a concentration of 20,000 cells/mL into the conduit and cultured them in a Ham's F-12 Nutrient Mixture (F12) (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (GIBCO) and 1% antibiotic-antimycotic (GIBCO) in an incubator at 37°C and 5% CO 2 .…”

Section: Membrane Characterizationmentioning

confidence: 99%

Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane

Peng¹,

Li²,

Chiu³

et al. 2017

IJN

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Section: Membrane Characterizationmentioning

confidence: 99%

Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane

Peng¹,

Li²,

Chiu³

et al. 2017

IJN

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“…NSCs were isolated and cultured according to the method described previously [39,40]. Briefly, mouse embryos at day 11.5 were collected from the pregnant FVB strain mice and placed in basal medium (a 1:1 mixture of Dulbecco's modified Eagle's medium high glucose/Ham's F12 medium [D/F12, Gibco] containing 100 units/ml penicillin and 100 mg/ml streptomycin).…”

Section: Isolation and Culture Of Nscsmentioning

confidence: 99%

Induction and regulation of differentiation in neural stem cells on ultra-nanocrystalline diamond films

et al. 2010

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“…Neural stem cells have been isolated from brain tissues according to the NSC-specific cell surface marker CD133 and NSCspecific genes, such as Sox1, Sox2, nestin, and FGF1 [54,55]. Thus, NSCs can be isolated using such approaches and then cultured to form neurospheres, which are indicators of self-renewal.…”

Section: Isolation and Characterization Of Neural Stem Cellsmentioning

confidence: 99%

“…Furthermore, the FGF-1B promoter-driven GFP reporter (F1B-GFP) (USA patent No. 6984 518; 7045 678; and 7745 214) was used to isolate NSCs from human [55] and mouse brains [54,55]. The F1B-GFP-selected NSCs exhibited significant repair efficacy in the damaged sciatic nerves of paraplegic rats.…”

Section: Isolation and Characterization Of Neural Stem Cellsmentioning

confidence: 99%

Neural Stem Cells and Induced Neurons for Nerve Injury Repair

Hsu¹,

Chen²,

Hsu³

et al. 2015

J Stem Trans Bio

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Cell transplantation can relieve the symptoms of or even reverse neurodegenerative diseases and repair nerve injuries. Fibroblast growth factor 1 promotes neuronal survival and stimulates axonal growth. A combination of fibroblast growth factor 1 and cell-based therapy is promising for nerve repair. Developers of future cell-based treatment should consider several key concerns: (1) the source of cells should be autologous, (2) consistent methods and protocols for cell isolation should be used, (3) the treatment should be tested in suitable animal models, and (4) the microenvironment of cells implanted should be optimally characterized. In addition, developing high temporal and spatial resolution images for cell tracking is crucial for evaluating the efficacy of cell transplantation. In this paper, we summarize recent progress in cellular reprogramming, such as induced neural stem cells and induced neurons, and the development of future cell-based therapy for peripheral nerve and spinal cord injury that includes conduits and growth factors. Fibroblast Growth Factor 1 for Nerve Injury RepairIn total, 22 mammalian fibroblast growth factors (FGFs) exist, grouped into seven subfamilies on the basis of differences in sequence homology and phylogeny. Notably, FGF1 and FGF2 share sequential and structural similarities and belong to the FGF1 subfamily [1]. The FGF ligands execute diverse functions by binding and activating the FGF receptor (FGFR) family of tyrosine kinase receptors with heparan sulfate proteoglycans. In total, four FGFR genes (FGFR1-FGFR4) encode receptors consisting of three extracellular immunoglobulin domains (D1-D3), a single-pass transmembrane domain, and a cytoplasmic tyrosine kinase domain [2]. Several FGFR isoforms exist because of exon skipping that removes the D1 domain and/or the acid box in FGFR1-FGFR4 [3]. Alternative splicing in the second half of the D3 domain of FGFR1-FGFR3 yields b (FGFR1b-FGFR3b) and c (FGFR1c-FGFR3c) isoforms that have distinct FGFbinding specificities [4] and are predominantly present in epithelial and mesenchymal cells, respectively. Each FGF binds to epithelial or mesenchymal FGFRs, with the exception of FGF1, which activates both spliced isoforms [3].The involvement of FGF signaling in human diseases has been thoroughly documented. Deregulated FGF signaling can contribute to pathological conditions through gain-or loss-of-function mutations in the FGFRs. Because both Fgf1 −/− and Fgf1mice are fertile and apparently normal [5], the physiological roles of FGF1 and FGF2 remain to be explored. However, FGF1 and FGF2 likely play a physiological role in the maintenance of the vascular tone because FGF1 and FGF2 administration lowers the blood pressure in rats [6] and can restore the nitric oxide synthase activity in spontaneously hypertensive rats [7]. In addition, blood vessels isolated from Fgf2 −/− mice exhibit a reduced response to vasoconstrictors. Although Fgf2 −/− mice had hypotension caused by reduced smooth muscle contractility [8], their blood pressu...

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Brain‐specific 1B promoter of FGF1 gene facilitates the isolation of neural stem/progenitor cells with self‐renewal and multipotent capacities

Cited by 52 publications

References 65 publications

Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane

Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane

Induction and regulation of differentiation in neural stem cells on ultra-nanocrystalline diamond films

Neural Stem Cells and Induced Neurons for Nerve Injury Repair

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